Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection

Detalhes bibliográficos
Autor(a) principal: Bozzi, Adriana
Data de Publicação: 2019
Outros Autores: Sayed, Nazish, Matsa, Elena, Sass, Gabriele, Neofytou, Evgenios, Clemons, Karl V., Oliveira, Rodrigo Corrêa de, Stevens, David A., Wu, Joseph C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34923
Resumo: Stanford Cardiovascular Institute. Stanford, CA, USA/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
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spelling Bozzi, AdrianaSayed, NazishMatsa, ElenaSass, GabrieleNeofytou, EvgeniosClemons, Karl V.Oliveira, Rodrigo Corrêa deStevens, David A.Wu, Joseph C2019-08-21T14:07:21Z2019-08-21T14:07:21Z2019BOZZI, Adriana et al. Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection. Stem Cell Reports, v. 12, n. 6, p. 1232-1241, 20192213-6711https://www.arca.fiocruz.br/handle/icict/3492310.1016/j.stemcr.2019.04.017engCell PressUsing Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleStanford Cardiovascular Institute. Stanford, CA, USA/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilStanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USAStanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USADivision of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USAStanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USADivision of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USAFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilDivision of Infectious Diseases and Geographic Medicine. Stanford University. School of Medicine, Stanford, CA, USA/California Institute for Medical Research. San Jose, CA, USAStanford Cardiovascular Institute. Stanford, CA, USA/Department of Medicine. Division of Cardiovascular Medicine. Stanford University. School of Medicine., Stanford, CA, USA/Department of Radiology. Stanford University. School of Medicine. Stanford, CA, USAChagas disease (ChD) is one of the most neglected tropical diseases, with cardiomyopathy being the main cause of death in Trypanosoma cruzi-infected patients. As the parasite actively replicates in cardiomyocytes (CMs), the heart remains a key target organ in the pathogenesis of ChD. Here we modeled ChD using human induced pluripotent stem cell-derived CMs (iPSC-CMs) to understand the complex interplay between the parasite and host cells. We showed that iPSC-CMs can get infected with the T. cruzi Y strain and that all parasite cycle stages can be identified in our model system. Importantly, characterization of T. cruzi-infected iPSC-CMs showed significant changes in their gene expression profile, cell contractility, and distribution of key cardiac markers. Moreover, these infected iPSC-CMs exhibited a pro-inflammatory profile as indicated by significantly elevated cytokine levels and cell-trafficking regulators. We believe our iPSC-CM model is a valuable platform to explore new treatment strategies for ChD.induced pluripotent stem cellsTrypanosoma cruziChagas diseasecardiomyopathydisease modelingdrug screeninginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/34923/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALUsing Human Induced Pluripotent Stem .pdfUsing Human Induced Pluripotent Stem .pdfapplication/pdf1908077https://www.arca.fiocruz.br/bitstream/icict/34923/2/Using%20Human%20Induced%20Pluripotent%20Stem%20.pdf178f6efbded1fadb06946707daced99aMD52TEXTUsing Human Induced Pluripotent Stem .pdf.txtUsing Human Induced Pluripotent Stem .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
title Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
spellingShingle Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
Bozzi, Adriana
induced pluripotent stem cells
Trypanosoma cruzi
Chagas disease
cardiomyopathy
disease modeling
drug screening
title_short Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
title_full Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
title_fullStr Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
title_full_unstemmed Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
title_sort Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection
author Bozzi, Adriana
author_facet Bozzi, Adriana
Sayed, Nazish
Matsa, Elena
Sass, Gabriele
Neofytou, Evgenios
Clemons, Karl V.
Oliveira, Rodrigo Corrêa de
Stevens, David A.
Wu, Joseph C
author_role author
author2 Sayed, Nazish
Matsa, Elena
Sass, Gabriele
Neofytou, Evgenios
Clemons, Karl V.
Oliveira, Rodrigo Corrêa de
Stevens, David A.
Wu, Joseph C
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bozzi, Adriana
Sayed, Nazish
Matsa, Elena
Sass, Gabriele
Neofytou, Evgenios
Clemons, Karl V.
Oliveira, Rodrigo Corrêa de
Stevens, David A.
Wu, Joseph C
dc.subject.en.pt_BR.fl_str_mv induced pluripotent stem cells
Trypanosoma cruzi
Chagas disease
cardiomyopathy
disease modeling
drug screening
topic induced pluripotent stem cells
Trypanosoma cruzi
Chagas disease
cardiomyopathy
disease modeling
drug screening
description Stanford Cardiovascular Institute. Stanford, CA, USA/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-21T14:07:21Z
dc.date.available.fl_str_mv 2019-08-21T14:07:21Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOZZI, Adriana et al. Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection. Stem Cell Reports, v. 12, n. 6, p. 1232-1241, 2019
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34923
dc.identifier.issn.pt_BR.fl_str_mv 2213-6711
dc.identifier.doi.none.fl_str_mv 10.1016/j.stemcr.2019.04.017
identifier_str_mv BOZZI, Adriana et al. Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes as a Model to Study Trypanosoma cruzi Infection. Stem Cell Reports, v. 12, n. 6, p. 1232-1241, 2019
2213-6711
10.1016/j.stemcr.2019.04.017
url https://www.arca.fiocruz.br/handle/icict/34923
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34923/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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