Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10213 |
Resumo: | Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, Brasil |
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Araújo, Cristina POsório, Ana Luiza A. RJorge, Klaudia S. GRamos, Carlos A. NSouza Filho, Antonio FVidal, Carlos E. SVargas, Agueda P. CRoxo, ElianaRocha, Adalgiza SSuffys, Philip NoelFonseca Jr., Antônio ASilva, Marcio RBarbosa Neto, José DCerqueira, Valíria DAraújo, Flábio R2015-05-04T17:07:31Z2015-05-04T17:07:31Z2014ARAÚJO, Cristina P. et al. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, v.45, n.2,p.633-640, 2014.1678-4405https://www.arca.fiocruz.br/handle/icict/10213porSociedade Brasileira de MicrobiologiaInspeção SanitáriaTuberculose BovinaBovine and bubaline tuberculosisNested-PCRReal-time PCRTissueSanitary inspectionTuberculosis BovinaInspección SanitariaCiências BiológicasInspeção SanitáriaTuberculose BovinaDirect detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, BrasilUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, BrasilUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, BrasilUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, BrasilUniversidade Federal de Santa Maria. Programa de Pós-Graduação em Medicina Veterinária. Santa Maria, RS, Brasil.Universidade Federal de Santa Maria. Programa de Pós-Graduação em Medicina Veterinária. Santa Maria, RS, BrasilInstituto Biológico de São Paulo, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Laboratório Nacional Agropecuário, Pedro Leopoldo, MG, Brasil.Embrapa Gado de Leite. Juiz de Fora, MG, Brasil.Universidade Federal do Pará. Castanhal, PA, Brasil.Universidade Federal do Pará. Castanhal, PA, Brasil.Embrapa Gado de Corte. Campo Grande, MS, Brasil.Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10213/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALadalgiza_rochaetal_IOC_2014.pdfapplication/pdf1284708https://www.arca.fiocruz.br/bitstream/icict/10213/2/adalgiza_rochaetal_IOC_2014.pdfa949193d633eb8179d819f773195fe4aMD52TEXTadalgiza_rochaetal_IOC_2014.pdf.txtadalgiza_rochaetal_IOC_2014.pdf.txtExtracted texttext/plain37379https://www.arca.fiocruz.br/bitstream/icict/10213/3/adalgiza_rochaetal_IOC_2014.pdf.txt9ce3ac4696e47416913498b40d96c339MD53icict/102132022-06-24 12:17:35.265oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| title |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| spellingShingle |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR Araújo, Cristina P Inspeção Sanitária Tuberculose Bovina Bovine and bubaline tuberculosis Nested-PCR Real-time PCR Tissue Sanitary inspection Tuberculosis Bovina Inspección Sanitaria Ciências Biológicas Inspeção Sanitária Tuberculose Bovina |
| title_short |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| title_full |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| title_fullStr |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| title_full_unstemmed |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| title_sort |
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
| author |
Araújo, Cristina P |
| author_facet |
Araújo, Cristina P Osório, Ana Luiza A. R Jorge, Klaudia S. G Ramos, Carlos A. N Souza Filho, Antonio F Vidal, Carlos E. S Vargas, Agueda P. C Roxo, Eliana Rocha, Adalgiza S Suffys, Philip Noel Fonseca Jr., Antônio A Silva, Marcio R Barbosa Neto, José D Cerqueira, Valíria D Araújo, Flábio R |
| author_role |
author |
| author2 |
Osório, Ana Luiza A. R Jorge, Klaudia S. G Ramos, Carlos A. N Souza Filho, Antonio F Vidal, Carlos E. S Vargas, Agueda P. C Roxo, Eliana Rocha, Adalgiza S Suffys, Philip Noel Fonseca Jr., Antônio A Silva, Marcio R Barbosa Neto, José D Cerqueira, Valíria D Araújo, Flábio R |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Araújo, Cristina P Osório, Ana Luiza A. R Jorge, Klaudia S. G Ramos, Carlos A. N Souza Filho, Antonio F Vidal, Carlos E. S Vargas, Agueda P. C Roxo, Eliana Rocha, Adalgiza S Suffys, Philip Noel Fonseca Jr., Antônio A Silva, Marcio R Barbosa Neto, José D Cerqueira, Valíria D Araújo, Flábio R |
| dc.subject.other.pt_BR.fl_str_mv |
Inspeção Sanitária Tuberculose Bovina |
| topic |
Inspeção Sanitária Tuberculose Bovina Bovine and bubaline tuberculosis Nested-PCR Real-time PCR Tissue Sanitary inspection Tuberculosis Bovina Inspección Sanitaria Ciências Biológicas Inspeção Sanitária Tuberculose Bovina |
| dc.subject.en.pt_BR.fl_str_mv |
Bovine and bubaline tuberculosis Nested-PCR Real-time PCR Tissue Sanitary inspection |
| dc.subject.es.pt_BR.fl_str_mv |
Tuberculosis Bovina Inspección Sanitaria Ciências Biológicas |
| dc.subject.decs.pt_BR.fl_str_mv |
Inspeção Sanitária Tuberculose Bovina |
| description |
Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal, Campo Grande, MS, Brasil |
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2014 |
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2014 |
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2015-05-04T17:07:31Z |
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2015-05-04T17:07:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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ARAÚJO, Cristina P. et al. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, v.45, n.2,p.633-640, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10213 |
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1678-4405 |
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ARAÚJO, Cristina P. et al. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, v.45, n.2,p.633-640, 2014. 1678-4405 |
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Sociedade Brasileira de Microbiologia |
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Sociedade Brasileira de Microbiologia |
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