Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes

Detalhes bibliográficos
Autor(a) principal: Cardoso, Alexander M.
Data de Publicação: 2012
Outros Autores: Cavalcante, Janaína J. V., Cantão, Maurício E., Thompson, Claudia E., Flatschart, Roberto B., Glogauer, Arnaldo, Scapin, Sandra M. N., Sade, Youssef B., Beltrão, Paulo J. M. S. I., Gerber, Alexandra L., Martins, Orlando B., Garcia, Eloi S., Souza, Wanderley de, Vasconcelos, Ana Tereza R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29930
Resumo: Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.
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spelling Cardoso, Alexander M.Cavalcante, Janaína J. V.Cantão, Maurício E.Thompson, Claudia E.Flatschart, Roberto B.Glogauer, ArnaldoScapin, Sandra M. N.Sade, Youssef B.Beltrão, Paulo J. M. S. I.Gerber, Alexandra L.Martins, Orlando B.Garcia, Eloi S.Souza, Wanderley deVasconcelos, Ana Tereza R.2018-11-08T11:27:51Z2018-11-08T11:27:51Z2012CARDOSO, Alexander M. et al. Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes. Plos One, v.7, n.11, e48505, 12p, Nov. 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/2993010.1371/journal.pone.0048505engPublic Library of ScienceAnálise MetagenômicaMicrobiotaCaracol InvasivoReservatórioMetagenomic AnalysisMicrobiotaReservoirInvasive SnailMetagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, Rio de Janeiro, Brasil / Empresa Brasileira de Pesquisa Agropecuária - CNPSA Concórdia, SC, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, Rio de Janeiro, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Laboratório Nacional de Computação Científica. Petrópolis, Rio de Janeiro, Brasil.The shortage of petroleum reserves and the increase in CO2 emissions have raised global concerns and highlighted the importance of adopting sustainable energy sources. Second-generation ethanol made from lignocellulosic materials is considered to be one of the most promising fuels for vehicles. The giant snail Achatina fulica is an agricultural pest whose biotechnological potential has been largely untested. Here, the composition of the microbial population within the crop of this invasive land snail, as well as key genes involved in various biochemical pathways, have been explored for the first time. In a high-throughput approach, 318 Mbp of 454-Titanium shotgun metagenomic sequencing data were obtained. The predominant bacterial phylum found was Proteobacteria, followed by Bacteroidetes and Firmicutes. Viruses, Fungi, and Archaea were present to lesser extents. The functional analysis reveals a variety of microbial genes that could assist the host in the degradation of recalcitrant lignocellulose, detoxification of xenobiotics, and synthesis of essential amino acids and vitamins, contributing to the adaptability and wide-ranging diet of this snail. More than 2,700 genes encoding glycoside hydrolase (GH) domains and carbohydrate-binding modules were detected. When we compared GH profiles, we found an abundance of sequences coding for oligosaccharide-degrading enzymes (36%), very similar to those from wallabies and giant pandas, as well as many novel cellulase and hemicellulase coding sequences, which points to this model as a remarkable potential source of enzymes for the biofuel industry. Furthermore, this work is a major step toward the understanding of the unique genetic profile of the land snail holobiont.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29930/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALelois_garcia_etal_IOC_2012.pdfelois_garcia_etal_IOC_2012.pdfapplication/pdf816444https://www.arca.fiocruz.br/bitstream/icict/29930/2/elois_garcia_etal_IOC_2012.pdf4691d985c5ff4ba98aae65423bceccdfMD52TEXTelois_garcia_etal_IOC_2012.pdf.txtelois_garcia_etal_IOC_2012.pdf.txtExtracted texttext/plain60230https://www.arca.fiocruz.br/bitstream/icict/29930/3/elois_garcia_etal_IOC_2012.pdf.txt33ecab9a2d09d00b7f82508864b44516MD53icict/299302018-11-09 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dc.title.pt_BR.fl_str_mv Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
title Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
spellingShingle Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
Cardoso, Alexander M.
Análise Metagenômica
Microbiota
Caracol Invasivo
Reservatório
Metagenomic Analysis
Microbiota
Reservoir
Invasive Snail
title_short Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
title_full Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
title_fullStr Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
title_full_unstemmed Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
title_sort Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes
author Cardoso, Alexander M.
author_facet Cardoso, Alexander M.
Cavalcante, Janaína J. V.
Cantão, Maurício E.
Thompson, Claudia E.
Flatschart, Roberto B.
Glogauer, Arnaldo
Scapin, Sandra M. N.
Sade, Youssef B.
Beltrão, Paulo J. M. S. I.
Gerber, Alexandra L.
Martins, Orlando B.
Garcia, Eloi S.
Souza, Wanderley de
Vasconcelos, Ana Tereza R.
author_role author
author2 Cavalcante, Janaína J. V.
Cantão, Maurício E.
Thompson, Claudia E.
Flatschart, Roberto B.
Glogauer, Arnaldo
Scapin, Sandra M. N.
Sade, Youssef B.
Beltrão, Paulo J. M. S. I.
Gerber, Alexandra L.
Martins, Orlando B.
Garcia, Eloi S.
Souza, Wanderley de
Vasconcelos, Ana Tereza R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cardoso, Alexander M.
Cavalcante, Janaína J. V.
Cantão, Maurício E.
Thompson, Claudia E.
Flatschart, Roberto B.
Glogauer, Arnaldo
Scapin, Sandra M. N.
Sade, Youssef B.
Beltrão, Paulo J. M. S. I.
Gerber, Alexandra L.
Martins, Orlando B.
Garcia, Eloi S.
Souza, Wanderley de
Vasconcelos, Ana Tereza R.
dc.subject.other.pt_BR.fl_str_mv Análise Metagenômica
Microbiota
Caracol Invasivo
Reservatório
topic Análise Metagenômica
Microbiota
Caracol Invasivo
Reservatório
Metagenomic Analysis
Microbiota
Reservoir
Invasive Snail
dc.subject.en.pt_BR.fl_str_mv Metagenomic Analysis
Microbiota
Reservoir
Invasive Snail
description Instituto Nacional de Metrologia, Qualidade e Tecnologia. Duque de Caxias, Rio de Janeiro, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2018-11-08T11:27:51Z
dc.date.available.fl_str_mv 2018-11-08T11:27:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARDOSO, Alexander M. et al. Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes. Plos One, v.7, n.11, e48505, 12p, Nov. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29930
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0048505
identifier_str_mv CARDOSO, Alexander M. et al. Metagenomic Analysis of the Microbiota from the Crop of an Invasive Snail Reveals a Rich Reservoir of Novel Genes. Plos One, v.7, n.11, e48505, 12p, Nov. 2012.
1932-6203
10.1371/journal.pone.0048505
url https://www.arca.fiocruz.br/handle/icict/29930
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/29930/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/29930/2/elois_garcia_etal_IOC_2012.pdf
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