Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/36335 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. |
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Medeiros, Nayara IngridPinto, Bruna FernandesSantos, Silvana Maria ElóiCarvalho, Andréa Teixeira deMagalhães, Luísa M. D.Dutra, Walderez OrnelasOliveira, Rodrigo Corrêa deGomes, Juliana Assis S.2019-10-10T14:22:16Z2019-10-10T14:22:16Z2019MEDEIROS, Nayara Ingrid et al. Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease. Frontiers in Immunology, v. 10, p. 1-11, 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/3633510.3389/fimmu.2019.00800engFrontiers Research FoundationEvidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Propedêutica Complementar. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Inflammatory Cell Dynamics Section. Center for Cancer Research. National Institute of Health. NIH, Bethesda, MD, USA.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil.Background: Chagas cardiomyopathy is the main fibrosing myocarditis among known heart diseases. Development of cardiomyopathy has been related to extracellular matrix (ECM) remodeling, which are controlled by matrix metalloproteinases (MMPs) and cytokines, especially interleukin (IL)-1β. The convertion of 31KDa inactive precursor, the proIL-1β in 17KDa active IL-1β peptide, is controlled by caspase-1-dependent pathway, associated with inflammasomes. Other caspase-1 independent mechanisms mediated by proteases, especially as MMPs, have already been described. Methods: We evaluated IL-1β activation pathways in neutrophils and monocyte subsets from patients with different clinical forms of Chagas disease after T. cruzi antigen stimulation by multiparameter flow cytometry. Results: Our data demonstrated that Chagas patients with the indeterminate clinical form (IND) showed increased levels of IL-1β post-stimulation as well as increased expression of MMP-2, NLRP3, and CASP1, which are associated with the classical caspase-1-dependent pathway. Conversely, patients with the cardiac clinical form (CARD) showed increased IL-1β after stimulation associated with MMP-9 and alternative caspase-1-independent pathway. Conclusions: We suggest some distinct molecular mechanisms for production of IL-1β in innate immune cells from patients with different clinical forms of Chagas disease. MMP-2 and MMP-9 gelatinases are associated with distinct disease outcomes and IL-1β production.Chagas diseaseIL-1β activation pathwaysCaspase-1-independentChagasic cardiomyopathyChagasic cardiomyopathyGelatinasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/36335/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALEvidence of Different .pdfEvidence of Different .pdfapplication/pdf3085663https://www.arca.fiocruz.br/bitstream/icict/36335/2/Evidence%20of%20Different%20.pdff6266a1b26d8964e822c9c9eceb9fb36MD52TEXTEvidence of Different .pdf.txtEvidence of Different .pdf.txtExtracted texttext/plain46742https://www.arca.fiocruz.br/bitstream/icict/36335/3/Evidence%20of%20Different%20.pdf.txte8ad1ef447d6864e306e43ed0e6ba041MD53icict/363352019-10-14 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| dc.title.pt_BR.fl_str_mv |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| title |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| spellingShingle |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease Medeiros, Nayara Ingrid Chagas disease IL-1β activation pathways Caspase-1-independent Chagasic cardiomyopathy Chagasic cardiomyopathy Gelatinases |
| title_short |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| title_full |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| title_fullStr |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| title_full_unstemmed |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| title_sort |
Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease |
| author |
Medeiros, Nayara Ingrid |
| author_facet |
Medeiros, Nayara Ingrid Pinto, Bruna Fernandes Santos, Silvana Maria Elói Carvalho, Andréa Teixeira de Magalhães, Luísa M. D. Dutra, Walderez Ornelas Oliveira, Rodrigo Corrêa de Gomes, Juliana Assis S. |
| author_role |
author |
| author2 |
Pinto, Bruna Fernandes Santos, Silvana Maria Elói Carvalho, Andréa Teixeira de Magalhães, Luísa M. D. Dutra, Walderez Ornelas Oliveira, Rodrigo Corrêa de Gomes, Juliana Assis S. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Medeiros, Nayara Ingrid Pinto, Bruna Fernandes Santos, Silvana Maria Elói Carvalho, Andréa Teixeira de Magalhães, Luísa M. D. Dutra, Walderez Ornelas Oliveira, Rodrigo Corrêa de Gomes, Juliana Assis S. |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease IL-1β activation pathways Caspase-1-independent Chagasic cardiomyopathy Chagasic cardiomyopathy Gelatinases |
| topic |
Chagas disease IL-1β activation pathways Caspase-1-independent Chagasic cardiomyopathy Chagasic cardiomyopathy Gelatinases |
| description |
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. |
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2019 |
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2019-10-10T14:22:16Z |
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2019-10-10T14:22:16Z |
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2019 |
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info:eu-repo/semantics/article |
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publishedVersion |
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MEDEIROS, Nayara Ingrid et al. Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease. Frontiers in Immunology, v. 10, p. 1-11, 2019. |
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https://www.arca.fiocruz.br/handle/icict/36335 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2019.00800 |
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MEDEIROS, Nayara Ingrid et al. Evidence of Different IL-1 beta Activation Pathways in Innate Immune Cells From Indeterminate and Cardiac Patients With Chronic Chagas Disease. Frontiers in Immunology, v. 10, p. 1-11, 2019. 1664-3224 10.3389/fimmu.2019.00800 |
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