Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease

Santos, Fred Luciano Neves; Celedon, Paola Alejandra Fiorani; Zanchin, Nilson Ivo Tonin; Brasil, Tatiana de Arruda Campos; Foti, Leonardo; Souza, Wayner Vieira de; Silva, Edmilson Domingos; Gomes, Yara de Miranda; Krieger, Marco Aurélio

Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease

Detalhes bibliográficos
Autor(a) principal:	Santos, Fred Luciano Neves
Data de Publicação:	2016
Outros Autores:	Celedon, Paola Alejandra Fiorani, Zanchin, Nilson Ivo Tonin, Brasil, Tatiana de Arruda Campos, Foti, Leonardo, Souza, Wayner Vieira de, Silva, Edmilson Domingos, Gomes, Yara de Miranda, Krieger, Marco Aurélio
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/19302
Resumo:	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.

Metadados do item

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spelling	Santos, Fred Luciano NevesCeledon, Paola Alejandra FioraniZanchin, Nilson Ivo ToninBrasil, Tatiana de Arruda CamposFoti, LeonardoSouza, Wayner Vieira deSilva, Edmilson DomingosGomes, Yara de MirandaKrieger, Marco Aurélio2017-06-05T18:44:44Z2017-06-05T18:44:44Z2016SANTOS, F. L. N. et al. Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. PLOS ONE, v. 11, n. 8, p. e0161100, 12 ago. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1930210.1371/journal.pone.0161100engTrypanosoma cruziDoença de ChagasTestes SorológicosDiagnósticoAnticorpos, protozoários / imunologiaAntígenos , protozoários / imunologiaDoença de Chagas / diagnósticoEnsaio de Imunoadsorção Enzimática / métodosTrypanosoma cruzi / imunologiaPerformance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Molecular Biology Institute of Paraná. Curitiba, PR, Brazil / Fundação Oswaldo Cruz. Carlos Chagas Institute. Curitiba, PR, Brazil.The performance of serologic tests in chronic Chagas disease diagnosis largely depends on the type and quality of the antigen preparations that are used for detection of anti-Trypanosoma cruzi antibodies. Whole-cell T. cruzi extracts or recombinant proteins have shown variation in the performance and cross-reactivity. Synthetic chimeric proteins comprising fragments of repetitive amino acids of several different proteins have been shown to improve assay performances to detect Chagasic infections. Here, we describe the production of four chimeric T. cruzi proteins and the assessment of their performance for diagnostic purposes. Circular Dichroism spectra indicated the absence of well-defined secondary structures, while polydispersity evaluated by Dynamic Light Scattering revealed only minor aggregates in 50 mM carbonate-bicarbonate (pH 9.6), demonstrating that it is an appropriate buffering system for sensitizing microplates. Serum samples from T. cruzi-infected and non-infected individuals were used to assess the performance of these antigens for detecting antibodies against T. cruzi, using both enzyme-linked immunosorbent assay and a liquid bead array platform. Performance parameters (AUC, sensitivity, specificity, accuracy and J index) showed high diagnostic accuracy for all chimeric proteins for detection of specific anti-T. cruzi antibodies and differentiated seropositive individuals from those who were seronegative. Our data suggest that these four chimeric proteins are eligible for phase II studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19302/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27517281 2016 sant-per.oa.pdf27517281 2016 sant-per.oa.pdfapplication/pdf1612334https://www.arca.fiocruz.br/bitstream/icict/19302/2/27517281%202016%20sant-per.oa.pdfc83d9c498354f9aec2d0bd97ff18f458MD52TEXT27517281 2016 sant-per.oa.pdf.txt27517281 2016 sant-per.oa.pdf.txtExtracted texttext/plain46591https://www.arca.fiocruz.br/bitstream/icict/19302/3/27517281%202016%20sant-per.oa.pdf.txt6428f46e243ab505c5952d2f2e67670bMD53icict/193022019-04-02 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dc.title.pt_BR.fl_str_mv	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
title	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
spellingShingle	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease Santos, Fred Luciano Neves Trypanosoma cruzi Doença de Chagas Testes Sorológicos Diagnóstico Anticorpos, protozoários / imunologia Antígenos , protozoários / imunologia Doença de Chagas / diagnóstico Ensaio de Imunoadsorção Enzimática / métodos Trypanosoma cruzi / imunologia
title_short	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
title_full	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
title_fullStr	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
title_full_unstemmed	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
title_sort	Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
author	Santos, Fred Luciano Neves
author_facet	Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Brasil, Tatiana de Arruda Campos Foti, Leonardo Souza, Wayner Vieira de Silva, Edmilson Domingos Gomes, Yara de Miranda Krieger, Marco Aurélio
author_role	author
author2	Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Brasil, Tatiana de Arruda Campos Foti, Leonardo Souza, Wayner Vieira de Silva, Edmilson Domingos Gomes, Yara de Miranda Krieger, Marco Aurélio
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Santos, Fred Luciano Neves Celedon, Paola Alejandra Fiorani Zanchin, Nilson Ivo Tonin Brasil, Tatiana de Arruda Campos Foti, Leonardo Souza, Wayner Vieira de Silva, Edmilson Domingos Gomes, Yara de Miranda Krieger, Marco Aurélio
dc.subject.other.none.fl_str_mv	Trypanosoma cruzi Doença de Chagas Testes Sorológicos Diagnóstico
topic	Trypanosoma cruzi Doença de Chagas Testes Sorológicos Diagnóstico Anticorpos, protozoários / imunologia Antígenos , protozoários / imunologia Doença de Chagas / diagnóstico Ensaio de Imunoadsorção Enzimática / métodos Trypanosoma cruzi / imunologia
dc.subject.decs.none.fl_str_mv	Anticorpos, protozoários / imunologia Antígenos , protozoários / imunologia Doença de Chagas / diagnóstico Ensaio de Imunoadsorção Enzimática / métodos Trypanosoma cruzi / imunologia
description	Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
publishDate	2016
dc.date.issued.fl_str_mv	2016
dc.date.accessioned.fl_str_mv	2017-06-05T18:44:44Z
dc.date.available.fl_str_mv	2017-06-05T18:44:44Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SANTOS, F. L. N. et al. Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. PLOS ONE, v. 11, n. 8, p. e0161100, 12 ago. 2016.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/19302
dc.identifier.issn.pt_BR.fl_str_mv	1932-6203
dc.identifier.doi.pt_BR.fl_str_mv	10.1371/journal.pone.0161100
identifier_str_mv	SANTOS, F. L. N. et al. Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease. PLOS ONE, v. 11, n. 8, p. e0161100, 12 ago. 2016. 1932-6203 10.1371/journal.pone.0161100
url	https://www.arca.fiocruz.br/handle/icict/19302
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/19302/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/19302/2/27517281%202016%20sant-per.oa.pdf https://www.arca.fiocruz.br/bitstream/icict/19302/3/27517281%202016%20sant-per.oa.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease

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