Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35445 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil. |
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Silva, Rodrigo Nunes Rodrigues daMoreira, Daniely CorreaSoares, Isabela FerreiraLuca, Paula Melo deTotino, Paulo Renato RivasMorgado, Fernanda NazaréHenriques, Maria das Graças de OliveiraCandea, André Luis PeixotoSingh, BalwanGalinski, Mary R.Moreno, AlbertoFerreira, Joseli OliveiraLima Junior, Josué da Costa2019-09-10T13:42:59Z2019-09-10T13:42:59Z2019SILVA, Rodrigo Nunes Rodrigues da et al. Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9. Vaccine, v. 37, p. 306-313, 2019.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/3544510.1016/j.vaccine.2018.10.0161873-2518engElsevier 12 MonthsMaláriaEpítopo linear de células BEpitopos de Linfócito TImunógenos à base de peptídeosImunogenicidadeAntigenicidadeMalariaLinear B-cell epitopeT-cell epitopePeptide-based immunogensImmunogenicityAntigenicityEpitopos de Linfócito TImmunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ. Brasil.Emory University. YerkesNational Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA.Yerkes National Primate Research Center. Emory Vaccine Center. Division of Infectious Diseases. Emory University School of Medicine, Emory University, Atlanta, GA, USA..Yerkes National Primate Research Center. Emory Vaccine Center. Division of Infectious Diseases. Emory University School of Medicine, Emory University, Atlanta, GA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Plasmodium vivax Merozoite Surface Protein-9 (PvMSP-9) is a malaria vaccine candidate naturally immunogenic in humans and able to induce high antibody titers in animals when delivered as a recombinant protein. Recently, we identified the sequence EAAPENAEPVHENA (PvMSP9E795-A808) as the main linear B-cell epitope in naturally exposed individuals. However, the potential of PvMSP9E795-A808 as an immunogen in experimental animal models remained unexplored. Here we assess the immunogenicity of PvMSP9E795-A808 using synthetic peptides. The peptides tested in BALB/c mice include two repeats of the sequence EAAPENAEPVHENA tested alone (peptide RII), or linked to an autologous (PvMSP9 peptide pL; pLRII) or heterologous (p2 tetanus toxin universal T cell epitope; TTRII) T cell epitope. Immune responses were evaluated by ELISA, FLUOROSPOT, and indirect immunofluorescence. We show that all of the peptide constructs tested were immunogenic eliciting specific IgG antibodies at different levels, with a prevalence of IgG1 and IgG2. Animals immunized with synthetic peptides containing T cell epitopes (pLRII or TTRII) had more efficient antibody responses that resulted in higher antibody titers able to recognize the native protein by immunofluorescence. Relevantly, the frequency of IFN-γ secreting SFC elicited by immunization with TTRII synthetic peptide was comparable to that reported to the PvMSP9-Nt recombinant protein. Taken together, our study indicates that PvMSP9E795-A808 is highly immunogenic in mice and further studies to evaluate its value as promising vaccine target are warranted. Moreover, our study supports the critical role of CD4 T cell epitopes to enhance humoral responses induced by subunit based vaccines.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35445/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIsabelaSoares_PaulaMLuca_etal_IOC_2019.pdfIsabelaSoares_PaulaMLuca_etal_IOC_2019.pdfapplication/pdf1019639https://www.arca.fiocruz.br/bitstream/icict/35445/2/IsabelaSoares_PaulaMLuca_etal_IOC_2019.pdf3df9f0d3bb4d9789ad244a0d3248cddaMD52TEXTIsabelaSoares_PaulaMLuca_etal_IOC_2019.pdf.txtIsabelaSoares_PaulaMLuca_etal_IOC_2019.pdf.txtExtracted texttext/plain51892https://www.arca.fiocruz.br/bitstream/icict/35445/3/IsabelaSoares_PaulaMLuca_etal_IOC_2019.pdf.txt35d907012c3fe7e4177488e80f8b44d5MD53icict/354452023-09-14 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| dc.title.pt_BR.fl_str_mv |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| title |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| spellingShingle |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 Silva, Rodrigo Nunes Rodrigues da Malária Epítopo linear de células B Epitopos de Linfócito T Imunógenos à base de peptídeos Imunogenicidade Antigenicidade Malaria Linear B-cell epitope T-cell epitope Peptide-based immunogens Immunogenicity Antigenicity Epitopos de Linfócito T |
| title_short |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| title_full |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| title_fullStr |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| title_full_unstemmed |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| title_sort |
Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9 |
| author |
Silva, Rodrigo Nunes Rodrigues da |
| author_facet |
Silva, Rodrigo Nunes Rodrigues da Moreira, Daniely Correa Soares, Isabela Ferreira Luca, Paula Melo de Totino, Paulo Renato Rivas Morgado, Fernanda Nazaré Henriques, Maria das Graças de Oliveira Candea, André Luis Peixoto Singh, Balwan Galinski, Mary R. Moreno, Alberto Ferreira, Joseli Oliveira Lima Junior, Josué da Costa |
| author_role |
author |
| author2 |
Moreira, Daniely Correa Soares, Isabela Ferreira Luca, Paula Melo de Totino, Paulo Renato Rivas Morgado, Fernanda Nazaré Henriques, Maria das Graças de Oliveira Candea, André Luis Peixoto Singh, Balwan Galinski, Mary R. Moreno, Alberto Ferreira, Joseli Oliveira Lima Junior, Josué da Costa |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Rodrigo Nunes Rodrigues da Moreira, Daniely Correa Soares, Isabela Ferreira Luca, Paula Melo de Totino, Paulo Renato Rivas Morgado, Fernanda Nazaré Henriques, Maria das Graças de Oliveira Candea, André Luis Peixoto Singh, Balwan Galinski, Mary R. Moreno, Alberto Ferreira, Joseli Oliveira Lima Junior, Josué da Costa |
| dc.subject.other.pt_BR.fl_str_mv |
Malária Epítopo linear de células B Epitopos de Linfócito T Imunógenos à base de peptídeos Imunogenicidade Antigenicidade |
| topic |
Malária Epítopo linear de células B Epitopos de Linfócito T Imunógenos à base de peptídeos Imunogenicidade Antigenicidade Malaria Linear B-cell epitope T-cell epitope Peptide-based immunogens Immunogenicity Antigenicity Epitopos de Linfócito T |
| dc.subject.en.pt_BR.fl_str_mv |
Malaria Linear B-cell epitope T-cell epitope Peptide-based immunogens Immunogenicity Antigenicity |
| dc.subject.decs.pt_BR.fl_str_mv |
Epitopos de Linfócito T |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil. |
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2019 |
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2019-09-10T13:42:59Z |
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2019-09-10T13:42:59Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, Rodrigo Nunes Rodrigues da et al. Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9. Vaccine, v. 37, p. 306-313, 2019. |
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https://www.arca.fiocruz.br/handle/icict/35445 |
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0264-410X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.vaccine.2018.10.016 |
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1873-2518 |
| identifier_str_mv |
SILVA, Rodrigo Nunes Rodrigues da et al. Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9. Vaccine, v. 37, p. 306-313, 2019. 0264-410X 10.1016/j.vaccine.2018.10.016 1873-2518 |
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Elsevier 12 Months |
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Elsevier 12 Months |
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