Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37022 |
Resumo: | Coordenação de 363 Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à 364 Pesquisa do Estado de São Paulo (FAPESP; 2012/51220-8 and 2016/03654-0), and 365 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 302661/2015- 366 8). In Salvador by grants of: (CNPq; 473667/2012 and 311054/2014-50), Fundação de 367 Amparo à Pesquisa do Estado da Bahia (FAPESB; 2574/2013), and Fundação Nacional 368 para o desenvolvimento do Ensino Superior (FUNDADESP; 9600113). |
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Campos, Karoline RodriguesSantos, Fred Luciano NevesBrito, Vanessa da SilvaGonçalves, Noilson Lazaro SousaAraujo, Thessika Hialla AlmeidaCastro Filho, Bernardo GalvãoAraujo, Adele Caterino2019-11-13T13:10:39Z2019-11-13T13:10:39Z2019CAMPOS, Karoline Rodrigues et al. Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil. Journal of Clinical Microbiology, p. 1-35, Nov. 2019.0095-1137https://www.arca.fiocruz.br/handle/icict/3702210.1128/JCM.01384-19Coordenação de 363 Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à 364 Pesquisa do Estado de São Paulo (FAPESP; 2012/51220-8 and 2016/03654-0), and 365 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 302661/2015- 366 8). In Salvador by grants of: (CNPq; 473667/2012 and 311054/2014-50), Fundação de 367 Amparo à Pesquisa do Estado da Bahia (FAPESB; 2574/2013), and Fundação Nacional 368 para o desenvolvimento do Ensino Superior (FUNDADESP; 9600113).Adolfo Lutz Institute. Immunology Department. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Adolfo Lutz Institute. Immunology Department. São Paulo, SP, Brazil.Difficulties to confirm and discriminate human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) infections by serological Western Blotting (WB) assay (HTLV Blot 2.4, MP Biomedicals) has been reported in Brazil, mainly in HIV/AIDS patients, with a large number of WB-indeterminate and WB-positive but HTLV untypeable results. Nonetheless, the line immunoassay (LIA) (INNO-LIA HTLV-I/II, Fujirebio) was pointed to enhance specificity and sensitivity for confirming HTLV-1/2 infections. To add information concerning the improved ability of LIA in relation to WB when applied in samples of individuals from different risk-groups from Brazil, we performed the present study. Three groups were analyzed: group 1 [G1], 62 samples from HIV/AIDS patients from São Paulo-SP (48 WB-indeterminate + 14 HTLV); group 2 [G2], 24 samples from patients with hepatitis B or hepatitis C from São Paulo (21 WB-indeterminate + 3 HTLV; 17 HIV-seropositive), and group 3 [G3], 25 samples from HTLV out-patients clinic from Salvador-Bahia (16 WB-indeterminate + 9 HTLV; all HIV-seronegative). Overall, the LIA confirmed HTLV-1/2 infection (HTLV-1, HTLV-2 or HTLV) in 66.1% [G1], 83.3% [G2], and 76.0% [G3] of samples. Interestingly, the majority of WB-indeterminate results were confirmed by LIA as HTLV-2 in G1 and G2, but not in G3, in which the samples were defined as HTLV-1 or HTLV positives. These results agree with the virus types that circulate in such patients of different regions in Brazil, and emphasize the LIA as the best serological test for confirming HTLV-1 and HTLV-2 infections, independently of being applied in HTLV-monoinfected or HTLV-coinfected individuals.engAmerican Society for MicrobiologyDiagnósticoHTLVTestes sorológicos confirmatóriosBrasilDiagnosticHTLVConfirmatory serologic testsBrazilLine immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37022/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCampos, C.R. Line....pdfCampos, C.R. 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Line....pdf.txtExtracted texttext/plain60964https://www.arca.fiocruz.br/bitstream/icict/37022/3/Campos%2c%20C.R.%20Line....pdf.txta0f68b7089d185368e944e613f4e0da2MD53icict/370222019-12-19 09:47:57.867oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-12-19T12:47:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| title |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| spellingShingle |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil Campos, Karoline Rodrigues Diagnóstico HTLV Testes sorológicos confirmatórios Brasil Diagnostic HTLV Confirmatory serologic tests Brazil |
| title_short |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| title_full |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| title_fullStr |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| title_full_unstemmed |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| title_sort |
Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil |
| author |
Campos, Karoline Rodrigues |
| author_facet |
Campos, Karoline Rodrigues Santos, Fred Luciano Neves Brito, Vanessa da Silva Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Castro Filho, Bernardo Galvão Araujo, Adele Caterino |
| author_role |
author |
| author2 |
Santos, Fred Luciano Neves Brito, Vanessa da Silva Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Castro Filho, Bernardo Galvão Araujo, Adele Caterino |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Campos, Karoline Rodrigues Santos, Fred Luciano Neves Brito, Vanessa da Silva Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Castro Filho, Bernardo Galvão Araujo, Adele Caterino |
| dc.subject.other.pt_BR.fl_str_mv |
Diagnóstico HTLV Testes sorológicos confirmatórios Brasil |
| topic |
Diagnóstico HTLV Testes sorológicos confirmatórios Brasil Diagnostic HTLV Confirmatory serologic tests Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Diagnostic HTLV Confirmatory serologic tests Brazil |
| description |
Coordenação de 363 Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à 364 Pesquisa do Estado de São Paulo (FAPESP; 2012/51220-8 and 2016/03654-0), and 365 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 302661/2015- 366 8). In Salvador by grants of: (CNPq; 473667/2012 and 311054/2014-50), Fundação de 367 Amparo à Pesquisa do Estado da Bahia (FAPESB; 2574/2013), and Fundação Nacional 368 para o desenvolvimento do Ensino Superior (FUNDADESP; 9600113). |
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2019 |
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2019-11-13T13:10:39Z |
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2019-11-13T13:10:39Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CAMPOS, Karoline Rodrigues et al. Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil. Journal of Clinical Microbiology, p. 1-35, Nov. 2019. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/37022 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0095-1137 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.01384-19 |
| identifier_str_mv |
CAMPOS, Karoline Rodrigues et al. Line immunoassay for confirmation and discrimination of human T-cell lymphotropic virus infections in inconclusive western blot serum samples from Brazil. Journal of Clinical Microbiology, p. 1-35, Nov. 2019. 0095-1137 10.1128/JCM.01384-19 |
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American Society for Microbiology |
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American Society for Microbiology |
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