Oxidative stress fuels Trypanosoma cruzi infection in mice

Detalhes bibliográficos
Autor(a) principal: Paiva, Claudia N.
Data de Publicação: 2012
Outros Autores: Feijó, Daniel F., Dutra, Fabianno F., Carneiro, Vitor C., Freitas, Guilherme B., Alves, Letícia S., Mesquita, Jacilene, Fortes, Guilherme B., Figueiredo, Rodrigo T., Souza, Heitor S. P., Fantappié, Marcelo R., Lannes-Vieira, Joseli, Bozza, Marcelo T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15585
Resumo: Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.
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spelling Paiva, Claudia N.Feijó, Daniel F.Dutra, Fabianno F.Carneiro, Vitor C.Freitas, Guilherme B.Alves, Letícia S.Mesquita, JacileneFortes, Guilherme B.Figueiredo, Rodrigo T.Souza, Heitor S. P.Fantappié, Marcelo R.Lannes-Vieira, JoseliBozza, Marcelo T.2016-09-01T17:55:00Z2016-09-01T17:55:00Z2012PAIVA, Claudia N. et al. Oxidative stress fuels Trypanosoma cruzi infection in mice. The Journal of Clinical Investigation, v.122, n.7, p.2531-2542, July 2012.0021-9738https://www.arca.fiocruz.br/handle/icict/1558510.1172/JCI585251558-8238engAmerican Society for Clinical InvestigationEstresse OxidativoTrypanosoma cruziDoença de ChagasTrypanosoma cruziOxidative stress fuelsChagas diseaseOxidative stress fuels Trypanosoma cruzi infection in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Bioquímica Médica. Programa de Biotecnologia e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Ciências Biomédicas, Pólo de Xérem. Xerem, RJ, BrasilUniversidade Federal do Rio de Janeiro (UFRJ). Hospital Universitário. Serviço de Hematologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Bioquímica Médica. Programa de Biotecnologia e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia das Interações. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.Oxidative damage contributes to microbe elimination during macrophage respiratory burst. Nuclear factor, erythroid-derived 2, like 2 (NRF2) orchestrates antioxidant defenses, including the expression of heme-oxygenase-1 (HO-1). Unexpectedly, the activation of NRF2 and HO-1 reduces infection by a number of pathogens, although the mechanism responsible for this effect is largely unknown. We studied Trypanosoma cruzi infection in mice in which NRF2/HO-1 was induced with cobalt protoporphyrin (CoPP). CoPP reduced parasitemia and tissue parasitism, while an inhibitor of HO-1 activity increased T. cruzi parasitemia in blood. CoPP-induced effects did not depend on the adaptive immunity, nor were parasites directly targeted. We also found that CoPP reduced macrophage parasitism, which depended on NRF2 expression but not on classical mechanisms such as apoptosis of infected cells, induction of type I IFN, or NO. We found that exogenous expression of NRF2 or HO-1 also reduced macrophage parasitism. Several antioxidants, including NRF2 activators, reduced macrophage parasite burden, while pro-oxidants promoted it. Reducing the intracellular labile iron pool decreased parasitism, and antioxidants increased the expression of ferritin and ferroportin in infected macrophages. Ferrous sulfate reversed the CoPP-induced decrease in macrophage parasite burden and, given in vivo, reversed their protective effects. Our results indicate that oxidative stress contributes to parasite persistence in host tissues and open a new avenue for the development of anti-T. cruzi drugs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15585/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoseli_vieira_etal_IOC_2012.pdfjoseli_vieira_etal_IOC_2012.pdfapplication/pdf2532131https://www.arca.fiocruz.br/bitstream/icict/15585/2/joseli_vieira_etal_IOC_2012.pdf93f026df907b204a5d3f228c828d86dfMD52TEXTjoseli_vieira_etal_IOC_2012.pdf.txtjoseli_vieira_etal_IOC_2012.pdf.txtExtracted texttext/plain66560https://www.arca.fiocruz.br/bitstream/icict/15585/3/joseli_vieira_etal_IOC_2012.pdf.txt82362f353484ba1d01258781d42e6137MD53icict/155852018-04-02 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dc.title.pt_BR.fl_str_mv Oxidative stress fuels Trypanosoma cruzi infection in mice
title Oxidative stress fuels Trypanosoma cruzi infection in mice
spellingShingle Oxidative stress fuels Trypanosoma cruzi infection in mice
Paiva, Claudia N.
Estresse Oxidativo
Trypanosoma cruzi
Doença de Chagas
Trypanosoma cruzi
Oxidative stress fuels
Chagas disease
title_short Oxidative stress fuels Trypanosoma cruzi infection in mice
title_full Oxidative stress fuels Trypanosoma cruzi infection in mice
title_fullStr Oxidative stress fuels Trypanosoma cruzi infection in mice
title_full_unstemmed Oxidative stress fuels Trypanosoma cruzi infection in mice
title_sort Oxidative stress fuels Trypanosoma cruzi infection in mice
author Paiva, Claudia N.
author_facet Paiva, Claudia N.
Feijó, Daniel F.
Dutra, Fabianno F.
Carneiro, Vitor C.
Freitas, Guilherme B.
Alves, Letícia S.
Mesquita, Jacilene
Fortes, Guilherme B.
Figueiredo, Rodrigo T.
Souza, Heitor S. P.
Fantappié, Marcelo R.
Lannes-Vieira, Joseli
Bozza, Marcelo T.
author_role author
author2 Feijó, Daniel F.
Dutra, Fabianno F.
Carneiro, Vitor C.
Freitas, Guilherme B.
Alves, Letícia S.
Mesquita, Jacilene
Fortes, Guilherme B.
Figueiredo, Rodrigo T.
Souza, Heitor S. P.
Fantappié, Marcelo R.
Lannes-Vieira, Joseli
Bozza, Marcelo T.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paiva, Claudia N.
Feijó, Daniel F.
Dutra, Fabianno F.
Carneiro, Vitor C.
Freitas, Guilherme B.
Alves, Letícia S.
Mesquita, Jacilene
Fortes, Guilherme B.
Figueiredo, Rodrigo T.
Souza, Heitor S. P.
Fantappié, Marcelo R.
Lannes-Vieira, Joseli
Bozza, Marcelo T.
dc.subject.other.pt_BR.fl_str_mv Estresse Oxidativo
Trypanosoma cruzi
Doença de Chagas
topic Estresse Oxidativo
Trypanosoma cruzi
Doença de Chagas
Trypanosoma cruzi
Oxidative stress fuels
Chagas disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Oxidative stress fuels
Chagas disease
description Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Professor Paulo de Góes. Departamento de Imunologia. Laboratório de Inflamação e Imunidade. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-09-01T17:55:00Z
dc.date.available.fl_str_mv 2016-09-01T17:55:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PAIVA, Claudia N. et al. Oxidative stress fuels Trypanosoma cruzi infection in mice. The Journal of Clinical Investigation, v.122, n.7, p.2531-2542, July 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15585
dc.identifier.issn.pt_BR.fl_str_mv 0021-9738
dc.identifier.doi.none.fl_str_mv 10.1172/JCI58525
dc.identifier.eissn.none.fl_str_mv 1558-8238
identifier_str_mv PAIVA, Claudia N. et al. Oxidative stress fuels Trypanosoma cruzi infection in mice. The Journal of Clinical Investigation, v.122, n.7, p.2531-2542, July 2012.
0021-9738
10.1172/JCI58525
1558-8238
url https://www.arca.fiocruz.br/handle/icict/15585
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Clinical Investigation
publisher.none.fl_str_mv American Society for Clinical Investigation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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