Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2000000300004 |
Resumo: | A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed. |
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Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginaleAnaplasma marginaleELISAserological testscattleA rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed.Colégio Brasileiro de Patologia Animal - CBPA2000-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2000000300004Pesquisa Veterinária Brasileira v.20 n.3 2000reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2000000300004info:eu-repo/semantics/openAccessMadruga,Cláudio R.Marques,Ana Paula C.Leal,Cássia Rejane B.Carvalho,Cristiano M.E.Araújo,Flábio R.Kessler,Raul H.eng2000-09-15T00:00:00Zoai:scielo:S0100-736X2000000300004Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2000-09-15T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
title |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
spellingShingle |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale Madruga,Cláudio R. Anaplasma marginale ELISA serological tests cattle |
title_short |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
title_full |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
title_fullStr |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
title_full_unstemmed |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
title_sort |
Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale |
author |
Madruga,Cláudio R. |
author_facet |
Madruga,Cláudio R. Marques,Ana Paula C. Leal,Cássia Rejane B. Carvalho,Cristiano M.E. Araújo,Flábio R. Kessler,Raul H. |
author_role |
author |
author2 |
Marques,Ana Paula C. Leal,Cássia Rejane B. Carvalho,Cristiano M.E. Araújo,Flábio R. Kessler,Raul H. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Madruga,Cláudio R. Marques,Ana Paula C. Leal,Cássia Rejane B. Carvalho,Cristiano M.E. Araújo,Flábio R. Kessler,Raul H. |
dc.subject.por.fl_str_mv |
Anaplasma marginale ELISA serological tests cattle |
topic |
Anaplasma marginale ELISA serological tests cattle |
description |
A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2000000300004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2000000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2000000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.20 n.3 2000 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122227449593856 |