Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000300209 |
Resumo: | ABSTRACT: The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection. |
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Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, BrazilDetectionavian metapneumovirussubtype Awild birdsSão PauloBrazilwaterfowlsurveillanceaMPVwildlife animalsbirdsvirosesABSTRACT: The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection.Colégio Brasileiro de Patologia Animal - CBPA2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000300209Pesquisa Veterinária Brasileira v.39 n.3 2019reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6021info:eu-repo/semantics/openAccessRizotto,Laís S.Simão,Raphael M.Scagion,Guilherme P.Simasaki,Alessandra A.Caserta,Leonardo C.Benassi,Julia C.Arns,Clarice W.Ferreira,Helena L.eng2019-04-01T00:00:00Zoai:scielo:S0100-736X2019000300209Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2019-04-01T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
title |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
spellingShingle |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil Rizotto,Laís S. Detection avian metapneumovirus subtype A wild birds São Paulo Brazil waterfowl surveillance aMPV wildlife animals birds viroses |
title_short |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
title_full |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
title_fullStr |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
title_full_unstemmed |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
title_sort |
Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil |
author |
Rizotto,Laís S. |
author_facet |
Rizotto,Laís S. Simão,Raphael M. Scagion,Guilherme P. Simasaki,Alessandra A. Caserta,Leonardo C. Benassi,Julia C. Arns,Clarice W. Ferreira,Helena L. |
author_role |
author |
author2 |
Simão,Raphael M. Scagion,Guilherme P. Simasaki,Alessandra A. Caserta,Leonardo C. Benassi,Julia C. Arns,Clarice W. Ferreira,Helena L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rizotto,Laís S. Simão,Raphael M. Scagion,Guilherme P. Simasaki,Alessandra A. Caserta,Leonardo C. Benassi,Julia C. Arns,Clarice W. Ferreira,Helena L. |
dc.subject.por.fl_str_mv |
Detection avian metapneumovirus subtype A wild birds São Paulo Brazil waterfowl surveillance aMPV wildlife animals birds viroses |
topic |
Detection avian metapneumovirus subtype A wild birds São Paulo Brazil waterfowl surveillance aMPV wildlife animals birds viroses |
description |
ABSTRACT: The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000300209 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000300209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-6021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.39 n.3 2019 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122239445303296 |