Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay

Detalhes bibliográficos
Autor(a) principal: Acevedo, Ana Maria
Data de Publicação: 2012
Outros Autores: Villarreal, Laura Yaneth B., Richtzenhain, Leonardo José, Noda, Julia, Brandão, Paulo Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/42205
Resumo: A duplex RT-PCR assay is reported for the simultaneous detection of avian infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV), the causative agents of major diseases in poultry. The duplex RT-PCR assay optimized showed a detection limit of 10-3 (101 EID50/50m L for IBV and 100.5 EID50/50m L for aMPV, respectively when two viruses were mixed and 10-1 for each one separated (103 EID50/50m L for IBV and 102.5 EID50/50m L for aMPV, respectively. It was specific, sensitive and applicable for the rapid detection of these viruses in clinical samples. 
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network_name_str Brazilian Journal of Veterinary Research and Animal Science
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spelling Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assayDetecção do vírus da bronquite infecciosa das galinhas e do metapneumovírus aviário utilizando uma reação de transcrição reversa com reação em cadeia pela polimerase duplexIBVaMPVPCRDiagnosticIBV. aMPV. PCR. Diagnosis.A duplex RT-PCR assay is reported for the simultaneous detection of avian infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV), the causative agents of major diseases in poultry. The duplex RT-PCR assay optimized showed a detection limit of 10-3 (101 EID50/50m L for IBV and 100.5 EID50/50m L for aMPV, respectively when two viruses were mixed and 10-1 for each one separated (103 EID50/50m L for IBV and 102.5 EID50/50m L for aMPV, respectively. It was specific, sensitive and applicable for the rapid detection of these viruses in clinical samples.  Descreve-se um ensaio de duplex RT-PCR assay para a detecção simultânea do vírus da bronquite infecciosa das galinhas (IBV) e do metapneumovirus aviário (aMPV), agentes etiológicos de doenças de elevada importância em avicultura. A duplex RT-PCR otimizada mostrou um limiar de detecção de 10-3 (101 EID50/50m L para IBV e 100.5 EID50/50m L para aMPV, respectivamente, quando da combinação dos dois vírus e 10-1 para cada um dos vírus em separado(103 EID50/50m L para IBV e 102.5 EID50/50m L para aMPV, respectivamente. O ensaio foi demonstrado como específico, sensível e aplicável à rápida detecção destes vírus em amostras clínicas. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2012-06-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/4220510.11606/issn.1678-4456.v49i3p221-224Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 Núm. 3 (2012); 221-224Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 No. 3 (2012); 221-224Brazilian Journal of Veterinary Research and Animal Science; v. 49 n. 3 (2012); 221-224Brazilian Journal of Veterinary Research and Animal Science; V. 49 N. 3 (2012); 221-2241678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/42205/45878Acevedo, Ana MariaVillarreal, Laura Yaneth B.Richtzenhain, Leonardo JoséNoda, JuliaBrandão, Paulo Eduardoinfo:eu-repo/semantics/openAccess2020-06-23T04:08:19Zoai:revistas.usp.br:article/42205Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:14.155617Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
Detecção do vírus da bronquite infecciosa das galinhas e do metapneumovírus aviário utilizando uma reação de transcrição reversa com reação em cadeia pela polimerase duplex
title Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
spellingShingle Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
Acevedo, Ana Maria
IBV
aMPV
PCR
Diagnostic
IBV. aMPV. PCR. Diagnosis.
title_short Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
title_full Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
title_fullStr Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
title_full_unstemmed Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
title_sort Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay
author Acevedo, Ana Maria
author_facet Acevedo, Ana Maria
Villarreal, Laura Yaneth B.
Richtzenhain, Leonardo José
Noda, Julia
Brandão, Paulo Eduardo
author_role author
author2 Villarreal, Laura Yaneth B.
Richtzenhain, Leonardo José
Noda, Julia
Brandão, Paulo Eduardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Acevedo, Ana Maria
Villarreal, Laura Yaneth B.
Richtzenhain, Leonardo José
Noda, Julia
Brandão, Paulo Eduardo
dc.subject.por.fl_str_mv IBV
aMPV
PCR
Diagnostic
IBV. aMPV. PCR. Diagnosis.
topic IBV
aMPV
PCR
Diagnostic
IBV. aMPV. PCR. Diagnosis.
description A duplex RT-PCR assay is reported for the simultaneous detection of avian infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV), the causative agents of major diseases in poultry. The duplex RT-PCR assay optimized showed a detection limit of 10-3 (101 EID50/50m L for IBV and 100.5 EID50/50m L for aMPV, respectively when two viruses were mixed and 10-1 for each one separated (103 EID50/50m L for IBV and 102.5 EID50/50m L for aMPV, respectively. It was specific, sensitive and applicable for the rapid detection of these viruses in clinical samples. 
publishDate 2012
dc.date.none.fl_str_mv 2012-06-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/42205
10.11606/issn.1678-4456.v49i3p221-224
url https://www.revistas.usp.br/bjvras/article/view/42205
identifier_str_mv 10.11606/issn.1678-4456.v49i3p221-224
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/42205/45878
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 Núm. 3 (2012); 221-224
Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 No. 3 (2012); 221-224
Brazilian Journal of Veterinary Research and Animal Science; v. 49 n. 3 (2012); 221-224
Brazilian Journal of Veterinary Research and Animal Science; V. 49 N. 3 (2012); 221-224
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051562528342016

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