Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead

Detalhes bibliográficos
Autor(a) principal: Ferreira, Paulo Avelar
Data de Publicação: 2012
Outros Autores: Bomfeti, Cleide Aparecida, da Silva Júnior, Romildo, Soares, Bruno Lima, Soares, Cláudio Roberto Fonseca Sousa, Moreira, Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/11574
Resumo: The objective of this work was to evaluate the tolerance of Cupriavidus necator strains to zinc, cadmium, copper and lead, as well as to determine the symbiotic efficiency of the most tolerant ones in legume species suited for use in revegetation. Tolerance was evaluated in LB medium supplemented with 2.5, 5.0, 7.5, 10, 12.5 and 15 mmol L‑1 of ZnSO4.7H2O, CdSO4.8H2O, CuSO4.5H2O and PbCl2, respectively, in comparison to a control without metal. The symbiotic efficiency of the four C. necator most metal-tolerant strains (UFLA02‑71, UFLA02‑73, UFLA01‑659 and UFLA01‑663) was determined, and these strains were inoculated in the species: Leucaena leucocephala, Enterolobium contortisiliquum, Acacia mangium, Mimosa caesalpiniifolia, M. pudica, M. pigra and M. acutistipula. Leucaena leucocephala, M. pudica and M. caesalpiniifolia and the strains UFLA02‑71 and UFLA01‑659, which presented the high symbiotic efficiency, were evaluatedin pots with soil. UFLA02‑71 provided increments of 870% in shoot dry matter of M. caesalpiniifolia, and UFLA01‑659 provided 885% in M. pudica and 924% in L. leucocephala. These strains should be assessed for potential use in programs to restore degraded areas, since they showed high efficiency in nitrogen fixation and were competitive with indigenous rhizobia populations, besides being highly tolerant to heavy metals.
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spelling Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and leadEficiência simbiótica de estirpes de Cupriavidus necator tolerantes a zinco, cádmio, cobre e chumbodegraded areas; β‑proteobacteria; biological nitrogen fixation; heavy metals; revegetationáreas degradadas; β‑proteobactéria; fixação biológica de nitrogênio; metais pesados; revegetação.The objective of this work was to evaluate the tolerance of Cupriavidus necator strains to zinc, cadmium, copper and lead, as well as to determine the symbiotic efficiency of the most tolerant ones in legume species suited for use in revegetation. Tolerance was evaluated in LB medium supplemented with 2.5, 5.0, 7.5, 10, 12.5 and 15 mmol L‑1 of ZnSO4.7H2O, CdSO4.8H2O, CuSO4.5H2O and PbCl2, respectively, in comparison to a control without metal. The symbiotic efficiency of the four C. necator most metal-tolerant strains (UFLA02‑71, UFLA02‑73, UFLA01‑659 and UFLA01‑663) was determined, and these strains were inoculated in the species: Leucaena leucocephala, Enterolobium contortisiliquum, Acacia mangium, Mimosa caesalpiniifolia, M. pudica, M. pigra and M. acutistipula. Leucaena leucocephala, M. pudica and M. caesalpiniifolia and the strains UFLA02‑71 and UFLA01‑659, which presented the high symbiotic efficiency, were evaluatedin pots with soil. UFLA02‑71 provided increments of 870% in shoot dry matter of M. caesalpiniifolia, and UFLA01‑659 provided 885% in M. pudica and 924% in L. leucocephala. These strains should be assessed for potential use in programs to restore degraded areas, since they showed high efficiency in nitrogen fixation and were competitive with indigenous rhizobia populations, besides being highly tolerant to heavy metals.O objetivo deste trabalho foi avaliar a tolerância de estirpes de <i>Cupriavidus necator</i> a zinco, cádmio, cobre e chumbo, além de determinar a eficiência simbiótica das estirpes mais tolerantes em associação a espécies leguminosas com potencial para revegetação. A tolerância foi testada em meio LB, suplementado com 2,5; 5,0; 7,5; 10; 12,5 e 15 mmol L‑1 de ZnSO4.7H2O, CdSO4.8H2O, CuSO4.5H2O e PbCl2, respectivamente, em comparação ao controle sem adição de metal. Determinou-se a eficiência simbiótica das quatro estirpes de C. necator mais tolerantes aos metais avaliados (UFLA02‑71, UFLA02‑73, UFLA01‑659 e UFLA01‑663), as quais foram inoculadas nas espécies: Leucaena leucocephala, Enterolobium contortisiliquum, Acacia mangium, Mimosa caesalpiniifolia, M. pudica, M. pigra e M. acutistipula. Em vasos com solos, avaliaramse L. leucocephala, M. pudica e M. caesalpiniifolia e as estirpes UFLA01‑659 e UFLA02‑71, selecionadas na avaliação de eficiência simbiótica. A estirpe UFLA02‑71 proporcionou incrementos de matéria seca da parte aérea de 870% em M. caesalpiniifolia, enquanto que UFLA01‑659 proporcionou 885% em M. pudica e 924% em L. leucocephala. As estirpes UFLA01‑659 e UFLA02‑71, além da alta tolerância a metais pesados, apresentaram eficiência em fixar nitrogênio, em simbiose com essas leguminosas, em solos com rizóbios nativos capazes de nodulá‑las, e devem ser avaliadas quanto ao seu potencial de utilização em programas de recuperação de áreas degradadas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFAPEMIG e CNPqFerreira, Paulo AvelarBomfeti, Cleide Aparecidada Silva Júnior, RomildoSoares, Bruno LimaSoares, Cláudio Roberto Fonseca SousaMoreira, Fatima Maria de Souza2012-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/11574Pesquisa Agropecuaria Brasileira; v.47, n.1, jan. 2012; 85-95Pesquisa Agropecuária Brasileira; v.47, n.1, jan. 2012; 85-951678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/11574/6757https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11574/7139info:eu-repo/semantics/openAccess2012-06-17T12:18:05Zoai:ojs.seer.sct.embrapa.br:article/11574Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T12:18:05Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
Eficiência simbiótica de estirpes de Cupriavidus necator tolerantes a zinco, cádmio, cobre e chumbo
title Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
spellingShingle Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
Ferreira, Paulo Avelar
degraded areas; β‑proteobacteria; biological nitrogen fixation; heavy metals; revegetation
áreas degradadas; β‑proteobactéria; fixação biológica de nitrogênio; metais pesados; revegetação.
title_short Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
title_full Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
title_fullStr Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
title_full_unstemmed Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
title_sort Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead
author Ferreira, Paulo Avelar
author_facet Ferreira, Paulo Avelar
Bomfeti, Cleide Aparecida
da Silva Júnior, Romildo
Soares, Bruno Lima
Soares, Cláudio Roberto Fonseca Sousa
Moreira, Fatima Maria de Souza
author_role author
author2 Bomfeti, Cleide Aparecida
da Silva Júnior, Romildo
Soares, Bruno Lima
Soares, Cláudio Roberto Fonseca Sousa
Moreira, Fatima Maria de Souza
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
FAPEMIG e CNPq
dc.contributor.author.fl_str_mv Ferreira, Paulo Avelar
Bomfeti, Cleide Aparecida
da Silva Júnior, Romildo
Soares, Bruno Lima
Soares, Cláudio Roberto Fonseca Sousa
Moreira, Fatima Maria de Souza
dc.subject.por.fl_str_mv degraded areas; β‑proteobacteria; biological nitrogen fixation; heavy metals; revegetation
áreas degradadas; β‑proteobactéria; fixação biológica de nitrogênio; metais pesados; revegetação.
topic degraded areas; β‑proteobacteria; biological nitrogen fixation; heavy metals; revegetation
áreas degradadas; β‑proteobactéria; fixação biológica de nitrogênio; metais pesados; revegetação.
description The objective of this work was to evaluate the tolerance of Cupriavidus necator strains to zinc, cadmium, copper and lead, as well as to determine the symbiotic efficiency of the most tolerant ones in legume species suited for use in revegetation. Tolerance was evaluated in LB medium supplemented with 2.5, 5.0, 7.5, 10, 12.5 and 15 mmol L‑1 of ZnSO4.7H2O, CdSO4.8H2O, CuSO4.5H2O and PbCl2, respectively, in comparison to a control without metal. The symbiotic efficiency of the four C. necator most metal-tolerant strains (UFLA02‑71, UFLA02‑73, UFLA01‑659 and UFLA01‑663) was determined, and these strains were inoculated in the species: Leucaena leucocephala, Enterolobium contortisiliquum, Acacia mangium, Mimosa caesalpiniifolia, M. pudica, M. pigra and M. acutistipula. Leucaena leucocephala, M. pudica and M. caesalpiniifolia and the strains UFLA02‑71 and UFLA01‑659, which presented the high symbiotic efficiency, were evaluatedin pots with soil. UFLA02‑71 provided increments of 870% in shoot dry matter of M. caesalpiniifolia, and UFLA01‑659 provided 885% in M. pudica and 924% in L. leucocephala. These strains should be assessed for potential use in programs to restore degraded areas, since they showed high efficiency in nitrogen fixation and were competitive with indigenous rhizobia populations, besides being highly tolerant to heavy metals.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11574
url https://seer.sct.embrapa.br/index.php/pab/article/view/11574
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11574/6757
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11574/7139
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.47, n.1, jan. 2012; 85-95
Pesquisa Agropecuária Brasileira; v.47, n.1, jan. 2012; 85-95
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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