Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003 |
Resumo: | Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations. |
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Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae speciessymbiosisβ-proteobacteriaantibiotic resistanceBiological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations.Escola Superior de Agricultura "Luiz de Queiroz"2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003Scientia Agricola v.69 n.4 2012reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162012000400003info:eu-repo/semantics/openAccessFlorentino,Ligiane AparecidaJaramillo,Paula Marcela DuqueSilva,Karina BarrosoSilva,Jacqueline Savana daOliveira,Silvia Maria deMoreira,Fatima Maria de Souzaeng2016-12-20T00:00:00Zoai:scielo:S0103-90162012000400003Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-12-20T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
title |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
spellingShingle |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species Florentino,Ligiane Aparecida symbiosis β-proteobacteria antibiotic resistance |
title_short |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
title_full |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
title_fullStr |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
title_full_unstemmed |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
title_sort |
Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species |
author |
Florentino,Ligiane Aparecida |
author_facet |
Florentino,Ligiane Aparecida Jaramillo,Paula Marcela Duque Silva,Karina Barroso Silva,Jacqueline Savana da Oliveira,Silvia Maria de Moreira,Fatima Maria de Souza |
author_role |
author |
author2 |
Jaramillo,Paula Marcela Duque Silva,Karina Barroso Silva,Jacqueline Savana da Oliveira,Silvia Maria de Moreira,Fatima Maria de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Florentino,Ligiane Aparecida Jaramillo,Paula Marcela Duque Silva,Karina Barroso Silva,Jacqueline Savana da Oliveira,Silvia Maria de Moreira,Fatima Maria de Souza |
dc.subject.por.fl_str_mv |
symbiosis β-proteobacteria antibiotic resistance |
topic |
symbiosis β-proteobacteria antibiotic resistance |
description |
Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162012000400003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.69 n.4 2012 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936462874705920 |