Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species

Detalhes bibliográficos
Autor(a) principal: Florentino,Ligiane Aparecida
Data de Publicação: 2012
Outros Autores: Jaramillo,Paula Marcela Duque, Silva,Karina Barroso, Silva,Jacqueline Savana da, Oliveira,Silvia Maria de, Moreira,Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003
Resumo: Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations.
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spelling Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae speciessymbiosisβ-proteobacteriaantibiotic resistanceBiological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations.Escola Superior de Agricultura "Luiz de Queiroz"2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003Scientia Agricola v.69 n.4 2012reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162012000400003info:eu-repo/semantics/openAccessFlorentino,Ligiane AparecidaJaramillo,Paula Marcela DuqueSilva,Karina BarrosoSilva,Jacqueline Savana daOliveira,Silvia Maria deMoreira,Fatima Maria de Souzaeng2016-12-20T00:00:00Zoai:scielo:S0103-90162012000400003Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2016-12-20T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
title Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
spellingShingle Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
Florentino,Ligiane Aparecida
symbiosis
β-proteobacteria
antibiotic resistance
title_short Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
title_full Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
title_fullStr Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
title_full_unstemmed Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
title_sort Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species
author Florentino,Ligiane Aparecida
author_facet Florentino,Ligiane Aparecida
Jaramillo,Paula Marcela Duque
Silva,Karina Barroso
Silva,Jacqueline Savana da
Oliveira,Silvia Maria de
Moreira,Fatima Maria de Souza
author_role author
author2 Jaramillo,Paula Marcela Duque
Silva,Karina Barroso
Silva,Jacqueline Savana da
Oliveira,Silvia Maria de
Moreira,Fatima Maria de Souza
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Florentino,Ligiane Aparecida
Jaramillo,Paula Marcela Duque
Silva,Karina Barroso
Silva,Jacqueline Savana da
Oliveira,Silvia Maria de
Moreira,Fatima Maria de Souza
dc.subject.por.fl_str_mv symbiosis
β-proteobacteria
antibiotic resistance
topic symbiosis
β-proteobacteria
antibiotic resistance
description Biological nitrogen fixation, performed by diazotrophic bacteria, plays an important role in the maintenance of agricultural systems, as it contributes with significant amounts of the nitrogen (N) needed for plant growth, totally or partially exempting the use of industrial N fertilisers. Twenty-five bacterial strains isolated from nodules of Leucaena leucocephala and Phaseolus vulgaris trap plants were studied. These nodules were formed after inoculation with suspensions of soil samples collected close to the root system of Sesbania virgata. In previous studies, these bacteria were identified as Cupriavidus necator. This study aimed to evaluate the ability of these strains to fix N2 in the free-living state and to use carbon (C) sources; their resistance to antibiotics; growth in media with different pH values and salt concentrations and symbiotic efficiency with L. leucocephala and P. vulgaris. In each test, these strains were compared to C. taiwanensis LMG 19424T. Although a high variability regarding antibiotic resistance, salt tolerance and use of C sources were observed among the 25 C. necator strains, a large group behaved similar regarding salt tolerance (20 strains) and antibiotic resistance (22 strains). C. necator strains behaved in a different way of LMG 19424T. Only one of the 25 strains studied, UFLA02-69, was not able to establish symbiosis with its trap species, P. vulgaris. Only the strains LMG19424T and UFLA01-672 were efficient in symbiosis with L. leucocephala. The ability to use C sources, grow in different pHs and salt concentrations and resistance to several antibiotics, may grant high saprophytic competence and greater competitivity to these strains in relation to the native Leguminosae-nodulating bacterial populations, suggesting potential use in inoculant strain selection studies for legumes cultivated in soils with a wide range of pH and salt concentrations.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162012000400003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.69 n.4 2012
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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