Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines

Detalhes bibliográficos
Autor(a) principal: Silva, Letícia Flores da
Data de Publicação: 2017
Outros Autores: Guerra, Celito Crivellaro, Czermainski, Ana Beatriz Costa, Ferrari, Leonardo, Bergold, Ana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/23258
Resumo: The objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit:specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines.
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spelling Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red winesValidação de método cromatográfico para análises de rotina de trans-resveratrol e quercetina em vinhos tintosVitis vinifera; high-pressure liquid chromatography; polyphenol; quantificationVitis vinifera; cromatografia líquida de alta eficiência; polifenol; quantificaçãoThe objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit:specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines.O objetivo deste trabalho foi validar um método rápido, com baixo consumo de solventes, para a análise de trans-resveratrol e quercetina em vinhos tintos. Os vinhos foram elaborados por método clássico, a partir das cultivares de uva (Vitis vinifera) Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat e Tempranillo. As amostras foram filtradas e analisadas por cromatografia líquida de alta eficiência (CLAE) com detector de arranjo de diodos (DAD), em 306 e 371 nm, quanto ao trans-resveratrol e à quercetina, respectivamente. Utilizou-se uma coluna octilsilano, e a fase móvel foi composta por um gradiente de metanol, água e ácido fórmico. O método foi validado de acordo com as seguintes figuras de mérito: especificidade, linearidade, limite de detecção, limite de quantificação, precisão, exatidão e robustez. O método CLAE-DAD proposto pode ser implantado para análises de trans-resveratrol e quercetina em vinhos tintos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraApoio logístico: Magda Beatris Gatto Salvador, Embrapa Uva e VinhoCelso Guarani Ruiz de Oliveira, Embrapa Uva e VinhoGisele Eliane Perissutti, Embrapa Uva e VinhoRaul Luiz Ben, Embrapa Uva e VinhoAnevir Marin, Embrapa Uva e VinhoSilva, Letícia Flores daGuerra, Celito CrivellaroCzermainski, Ana Beatriz CostaFerrari, LeonardoBergold, Ana Maria2017-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/23258Pesquisa Agropecuaria Brasileira; v.52, n.5, maio 2017; 335-343Pesquisa Agropecuária Brasileira; v.52, n.5, maio 2017; 335-3431678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/23258/13763https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/23258/15292Direitos autorais 2017 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2017-06-22T19:48:26Zoai:ojs.seer.sct.embrapa.br:article/23258Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2017-06-22T19:48:26Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
Validação de método cromatográfico para análises de rotina de trans-resveratrol e quercetina em vinhos tintos
title Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
spellingShingle Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
Silva, Letícia Flores da
Vitis vinifera; high-pressure liquid chromatography; polyphenol; quantification
Vitis vinifera; cromatografia líquida de alta eficiência; polifenol; quantificação
title_short Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
title_full Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
title_fullStr Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
title_full_unstemmed Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
title_sort Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines
author Silva, Letícia Flores da
author_facet Silva, Letícia Flores da
Guerra, Celito Crivellaro
Czermainski, Ana Beatriz Costa
Ferrari, Leonardo
Bergold, Ana Maria
author_role author
author2 Guerra, Celito Crivellaro
Czermainski, Ana Beatriz Costa
Ferrari, Leonardo
Bergold, Ana Maria
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
Apoio logístico: Magda Beatris Gatto Salvador, Embrapa Uva e Vinho
Celso Guarani Ruiz de Oliveira, Embrapa Uva e Vinho
Gisele Eliane Perissutti, Embrapa Uva e Vinho
Raul Luiz Ben, Embrapa Uva e Vinho
Anevir Marin, Embrapa Uva e Vinho
dc.contributor.author.fl_str_mv Silva, Letícia Flores da
Guerra, Celito Crivellaro
Czermainski, Ana Beatriz Costa
Ferrari, Leonardo
Bergold, Ana Maria
dc.subject.por.fl_str_mv Vitis vinifera; high-pressure liquid chromatography; polyphenol; quantification
Vitis vinifera; cromatografia líquida de alta eficiência; polifenol; quantificação
topic Vitis vinifera; high-pressure liquid chromatography; polyphenol; quantification
Vitis vinifera; cromatografia líquida de alta eficiência; polifenol; quantificação
description The objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit:specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/23258
url https://seer.sct.embrapa.br/index.php/pab/article/view/23258
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/23258/13763
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/23258/15292
dc.rights.driver.fl_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2017 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.52, n.5, maio 2017; 335-343
Pesquisa Agropecuária Brasileira; v.52, n.5, maio 2017; 335-343
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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