Genetic diversity assessed by microsatellite markers in sweet corn cultivars.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035081 http://dx.doi.org/10.1590/0103-9016-2014-0307 |
Resumo: | Information on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the NJ tree. This study on genetic diversity will be useful for planning future studies on sweet corn genetic resources and can complement the breeding programs for this crop. |
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Genetic diversity assessed by microsatellite markers in sweet corn cultivars.Diversidade genéticaGermoplasmaMilhoInformation on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the NJ tree. This study on genetic diversity will be useful for planning future studies on sweet corn genetic resources and can complement the breeding programs for this crop.FLAVIA FRANCA TEIXEIRA, CNPMS.LOPES, A. D.SCAPIM, C. A.MACHADO, M. de F. P. da S.MANGOLIN, C. A.SILVA, T. A.CANTAGALI, L. B.TEIXEIRA, F. F.MORA, F.2016-01-26T11:11:11Z2016-01-26T11:11:11Z2016-01-2620152016-02-02T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientia Agrícola, Piracicaba, v. 72, n. 6, p. 513-519, nov./dez. 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035081http://dx.doi.org/10.1590/0103-9016-2014-0307porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:34:32Zoai:www.alice.cnptia.embrapa.br:doc/1035081Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:34:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:34:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
title |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
spellingShingle |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. LOPES, A. D. Diversidade genética Germoplasma Milho |
title_short |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
title_full |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
title_fullStr |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
title_full_unstemmed |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
title_sort |
Genetic diversity assessed by microsatellite markers in sweet corn cultivars. |
author |
LOPES, A. D. |
author_facet |
LOPES, A. D. SCAPIM, C. A. MACHADO, M. de F. P. da S. MANGOLIN, C. A. SILVA, T. A. CANTAGALI, L. B. TEIXEIRA, F. F. MORA, F. |
author_role |
author |
author2 |
SCAPIM, C. A. MACHADO, M. de F. P. da S. MANGOLIN, C. A. SILVA, T. A. CANTAGALI, L. B. TEIXEIRA, F. F. MORA, F. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
dc.contributor.author.fl_str_mv |
LOPES, A. D. SCAPIM, C. A. MACHADO, M. de F. P. da S. MANGOLIN, C. A. SILVA, T. A. CANTAGALI, L. B. TEIXEIRA, F. F. MORA, F. |
dc.subject.por.fl_str_mv |
Diversidade genética Germoplasma Milho |
topic |
Diversidade genética Germoplasma Milho |
description |
Information on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were recorded in the MG161-Branco Doce and Doce Cubano OPVs. The polymorphism information content ranged from 0.19 (Umc2319) to 0.71 (Umc2205). The analysis of molecular variance revealed that most of the genetic variability was concentrated within the cultivars of sweet corn (75 %), with less variability between them (25 %). The consensus tree derived from the neighbor-joining (NJ) algorithm using 1,000 bootstrapping replicates revealed seven genetically different groups. Nei?s diversity values varied between 0.103 (Doce do Hawai × CNPH-1 cultivars) and 0.645 (Amarelo Doce × Lili cultivars), indicating a narrow genetic basis. The Lili hybrid was the most distant cultivar, as revealed by Principal Coordinates Analysis and the NJ tree. This study on genetic diversity will be useful for planning future studies on sweet corn genetic resources and can complement the breeding programs for this crop. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-01-26T11:11:11Z 2016-01-26T11:11:11Z 2016-01-26 2016-02-02T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Scientia Agrícola, Piracicaba, v. 72, n. 6, p. 513-519, nov./dez. 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035081 http://dx.doi.org/10.1590/0103-9016-2014-0307 |
identifier_str_mv |
Scientia Agrícola, Piracicaba, v. 72, n. 6, p. 513-519, nov./dez. 2015. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035081 http://dx.doi.org/10.1590/0103-9016-2014-0307 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503416522735616 |