Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.

Detalhes bibliográficos
Autor(a) principal: PINTO, F. de O.
Data de Publicação: 2007
Outros Autores: MALUF, M. P., GUERREIRO-FILHO, O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022
Resumo: The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant
id EMBR_77373785c9e9cd9bb3ab0e6ed07ec0a9
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/881022
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.microsatellitesmolecular markersmicrossatélitesmarcadores molecularesLeucoptera CoffeellaThe objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significantFERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC.PINTO, F. de O.MALUF, M. P.GUERREIRO-FILHO, O.2011-04-09T22:29:21Z2011-04-09T22:29:21Z2011-03-1620072011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:32:21Zoai:www.alice.cnptia.embrapa.br:doc/881022Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:32:21falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:32:21Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
title Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
spellingShingle Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
PINTO, F. de O.
microsatellites
molecular markers
microssatélites
marcadores moleculares
Leucoptera Coffeella
title_short Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
title_full Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
title_fullStr Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
title_full_unstemmed Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
title_sort Study of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.
author PINTO, F. de O.
author_facet PINTO, F. de O.
MALUF, M. P.
GUERREIRO-FILHO, O.
author_role author
author2 MALUF, M. P.
GUERREIRO-FILHO, O.
author2_role author
author
dc.contributor.none.fl_str_mv FERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC.
dc.contributor.author.fl_str_mv PINTO, F. de O.
MALUF, M. P.
GUERREIRO-FILHO, O.
dc.subject.por.fl_str_mv microsatellites
molecular markers
microssatélites
marcadores moleculares
Leucoptera Coffeella
topic microsatellites
molecular markers
microssatélites
marcadores moleculares
Leucoptera Coffeella
description The objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significant
publishDate 2007
dc.date.none.fl_str_mv 2007
2011-04-09T22:29:21Z
2011-04-09T22:29:21Z
2011-03-16
2011-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022
identifier_str_mv Pesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503340836519936

Registros relacionados