Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037631 |
Resumo: | This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams. |
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Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams.animal healthThis study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.Ana P. C. SILVA, UFMG; Auricélio A. Macêdo, UFMG; Luciana F. Costa, UFMG; Cláudia E. Rocha, UFMG; Luize N. N. Garcia, UFMG; Jade R. D. Farias, UFMG; Priscilla P. R. Gomes, UFMG; Gustavo C. Teixeira, UFMG; Kessler W. J. Fonseca, UFMG; Andréa R. F. Maia, UFMG; Gabriela G. Neves, UFMG; Everton L. Romão, UFMG; Teane M. A. Silva, UFMG; Juliana P. S. Mol, UFMG; Renata M. Oliveira, Universidade Estadual do Maranhão; Márcio S. S. Araújo, Fundação Oswaldo Cruz, MG; Ernane F. Nascimento, UFMG; Olindo A. Martins-Filho, Fundação Oswaldo Cruz, MG; HUMBERTO DE MELLO BRANDAO, CNPGL; Tatiane A. Paixão, UFMG; Renato L. Santos, UFMG.SILVA, A. P. C.MACÊDO, A. A.COSTA, L. F.ROCHA, C. E.GARCIA, L. N. N.FARIAS, J. R. D.GOMES, P. P. R.TEIXEIRA, G. C.FONSECA, K. W. J.MAIA, A. R. F.NEVES, G. G.ROMÃO, E. L.SILVA, T. M. A.MOL, J. P. S.OLIVEIRA, R. M.ARAÚJO, M. S. S.NASCIMENTO, E. F.MARTINS-FILHO, O. A.BRANDAO, H. de M.PAIXÃO, T. A.SANTOS, R. L.2016-02-18T11:11:11Z2016-02-18T11:11:11Z2016-02-1820152016-02-19T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 10, n. 8, e013865, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037631enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:54:56Zoai:www.alice.cnptia.embrapa.br:doc/1037631Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:54:56falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:54:56Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
title |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
spellingShingle |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. SILVA, A. P. C. animal health |
title_short |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
title_full |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
title_fullStr |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
title_full_unstemmed |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
title_sort |
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams. |
author |
SILVA, A. P. C. |
author_facet |
SILVA, A. P. C. MACÊDO, A. A. COSTA, L. F. ROCHA, C. E. GARCIA, L. N. N. FARIAS, J. R. D. GOMES, P. P. R. TEIXEIRA, G. C. FONSECA, K. W. J. MAIA, A. R. F. NEVES, G. G. ROMÃO, E. L. SILVA, T. M. A. MOL, J. P. S. OLIVEIRA, R. M. ARAÚJO, M. S. S. NASCIMENTO, E. F. MARTINS-FILHO, O. A. BRANDAO, H. de M. PAIXÃO, T. A. SANTOS, R. L. |
author_role |
author |
author2 |
MACÊDO, A. A. COSTA, L. F. ROCHA, C. E. GARCIA, L. N. N. FARIAS, J. R. D. GOMES, P. P. R. TEIXEIRA, G. C. FONSECA, K. W. J. MAIA, A. R. F. NEVES, G. G. ROMÃO, E. L. SILVA, T. M. A. MOL, J. P. S. OLIVEIRA, R. M. ARAÚJO, M. S. S. NASCIMENTO, E. F. MARTINS-FILHO, O. A. BRANDAO, H. de M. PAIXÃO, T. A. SANTOS, R. L. |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Ana P. C. SILVA, UFMG; Auricélio A. Macêdo, UFMG; Luciana F. Costa, UFMG; Cláudia E. Rocha, UFMG; Luize N. N. Garcia, UFMG; Jade R. D. Farias, UFMG; Priscilla P. R. Gomes, UFMG; Gustavo C. Teixeira, UFMG; Kessler W. J. Fonseca, UFMG; Andréa R. F. Maia, UFMG; Gabriela G. Neves, UFMG; Everton L. Romão, UFMG; Teane M. A. Silva, UFMG; Juliana P. S. Mol, UFMG; Renata M. Oliveira, Universidade Estadual do Maranhão; Márcio S. S. Araújo, Fundação Oswaldo Cruz, MG; Ernane F. Nascimento, UFMG; Olindo A. Martins-Filho, Fundação Oswaldo Cruz, MG; HUMBERTO DE MELLO BRANDAO, CNPGL; Tatiane A. Paixão, UFMG; Renato L. Santos, UFMG. |
dc.contributor.author.fl_str_mv |
SILVA, A. P. C. MACÊDO, A. A. COSTA, L. F. ROCHA, C. E. GARCIA, L. N. N. FARIAS, J. R. D. GOMES, P. P. R. TEIXEIRA, G. C. FONSECA, K. W. J. MAIA, A. R. F. NEVES, G. G. ROMÃO, E. L. SILVA, T. M. A. MOL, J. P. S. OLIVEIRA, R. M. ARAÚJO, M. S. S. NASCIMENTO, E. F. MARTINS-FILHO, O. A. BRANDAO, H. de M. PAIXÃO, T. A. SANTOS, R. L. |
dc.subject.por.fl_str_mv |
animal health |
topic |
animal health |
description |
This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-02-18T11:11:11Z 2016-02-18T11:11:11Z 2016-02-18 2016-02-19T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 10, n. 8, e013865, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037631 |
identifier_str_mv |
Plos One, v. 10, n. 8, e013865, 2015. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037631 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503418115522560 |