Improving in vitro induction of autopolyploidy in grapevine seedless cultivars.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/979552 |
Resumo: | The efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants. |
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Improving in vitro induction of autopolyploidy in grapevine seedless cultivars.ViticulturaUvaGenéticaPoliploidiaThe efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants.IRACI SINSKI, CNPUV; DANIELA DAL BOSCO, CNPUV; Neiva Izabel Pierozzi, IAC; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV.SINSKI, I.BOSCO, D. D.PIEROZZI, N. I.MAIA, J. D. G.RITSCHEL, P. S.QUECINI, V.2016-02-23T06:52:15Z2016-02-23T06:52:15Z2014-02-1220142016-02-23T06:52:15Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEuphytica, Wageningen, v. 196, n. 2, p. 299-311, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/979552enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:23:43Zoai:www.alice.cnptia.embrapa.br:doc/979552Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:23:43falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:23:43Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
title |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
spellingShingle |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. SINSKI, I. Viticultura Uva Genética Poliploidia |
title_short |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
title_full |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
title_fullStr |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
title_full_unstemmed |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
title_sort |
Improving in vitro induction of autopolyploidy in grapevine seedless cultivars. |
author |
SINSKI, I. |
author_facet |
SINSKI, I. BOSCO, D. D. PIEROZZI, N. I. MAIA, J. D. G. RITSCHEL, P. S. QUECINI, V. |
author_role |
author |
author2 |
BOSCO, D. D. PIEROZZI, N. I. MAIA, J. D. G. RITSCHEL, P. S. QUECINI, V. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
IRACI SINSKI, CNPUV; DANIELA DAL BOSCO, CNPUV; Neiva Izabel Pierozzi, IAC; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
dc.contributor.author.fl_str_mv |
SINSKI, I. BOSCO, D. D. PIEROZZI, N. I. MAIA, J. D. G. RITSCHEL, P. S. QUECINI, V. |
dc.subject.por.fl_str_mv |
Viticultura Uva Genética Poliploidia |
topic |
Viticultura Uva Genética Poliploidia |
description |
The efficiency of in vitro polyploidization depends on several variables associated to the plant, the antimicrotubule agent and the interactions between them. In the present work, we have used response-surface methodology to determine the best operating conditions for plant recovery in polyploidization assays for shoot apices and somatic embryos of two seedless grape cultivars, employing colchicine and oryzalin. Explant type, tubulin-interfering compound and concentration were the critical factors determining plant recovery. Linear reduction in viable plant regeneration via organogenesis and somatic embryogenesis was obtained by increasing oryzalin concentrations and treatment time, whereas the effects of colchicine were better described by a quadratic design for both explants types. The conditions promoting higher rates of plant recovery were used in chromosome doubling experiments with oryzalin and colchicine for shoot apices and somatic embryos of ?Crimson seedless? and ?BRS Clara?. The established protocols allowed the recovery of non-chimerical autotetraploid plants at rates higher than 30 % for both cultivars. Stomata size parameters statistically correlate to the ploidy level of the regenerants and were effective for preliminary polyploidy screening. Autotetraploid lines of seedless grapes were incorporated into the Vitis germplasm bank for further agronomical evaluations. To our knowledge, this is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-12 2014 2016-02-23T06:52:15Z 2016-02-23T06:52:15Z 2016-02-23T06:52:15Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Euphytica, Wageningen, v. 196, n. 2, p. 299-311, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/979552 |
identifier_str_mv |
Euphytica, Wageningen, v. 196, n. 2, p. 299-311, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/979552 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503418593673216 |