Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.
Main Author: | |
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Publication Date: | 2010 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Download full: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/873059 |
Summary: | Sultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA. |
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Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.Uva sem sementeExpressão gênicaRDADesenvolvimento da bagaPCR em tempo realUva de mesaIdentificação genéticaViticulturaUvaBiologia molecularGenéticaSultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA.DANIELLE COSTENARO-DA-SILVA, UFRGS; GISELE PASSAIA, CNPUV (bolsista); JOÃO A. P. HENRIQUES, UFRGS; ROGÉRIO MARGIS, UFRGS; GIANCARLO PASQUALI, UFRGS; LUIS FERNANDO REVERS, CNPUV.COSTENARO-DA-SILVA, D.PASSAIA, G.HENRIQUES, J. A. P.MARGIS, R.PASQUALI, G.REVERS, L. F.2019-04-27T00:41:45Z2019-04-27T00:41:45Z2011-01-1420102019-05-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article510-519Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/873059enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-04-27T00:41:54Zoai:www.alice.cnptia.embrapa.br:doc/873059Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-04-27T00:41:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-04-27T00:41:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
title |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
spellingShingle |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. COSTENARO-DA-SILVA, D. Uva sem semente Expressão gênica RDA Desenvolvimento da baga PCR em tempo real Uva de mesa Identificação genética Viticultura Uva Biologia molecular Genética |
title_short |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
title_full |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
title_fullStr |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
title_full_unstemmed |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
title_sort |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
author |
COSTENARO-DA-SILVA, D. |
author_facet |
COSTENARO-DA-SILVA, D. PASSAIA, G. HENRIQUES, J. A. P. MARGIS, R. PASQUALI, G. REVERS, L. F. |
author_role |
author |
author2 |
PASSAIA, G. HENRIQUES, J. A. P. MARGIS, R. PASQUALI, G. REVERS, L. F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
DANIELLE COSTENARO-DA-SILVA, UFRGS; GISELE PASSAIA, CNPUV (bolsista); JOÃO A. P. HENRIQUES, UFRGS; ROGÉRIO MARGIS, UFRGS; GIANCARLO PASQUALI, UFRGS; LUIS FERNANDO REVERS, CNPUV. |
dc.contributor.author.fl_str_mv |
COSTENARO-DA-SILVA, D. PASSAIA, G. HENRIQUES, J. A. P. MARGIS, R. PASQUALI, G. REVERS, L. F. |
dc.subject.por.fl_str_mv |
Uva sem semente Expressão gênica RDA Desenvolvimento da baga PCR em tempo real Uva de mesa Identificação genética Viticultura Uva Biologia molecular Genética |
topic |
Uva sem semente Expressão gênica RDA Desenvolvimento da baga PCR em tempo real Uva de mesa Identificação genética Viticultura Uva Biologia molecular Genética |
description |
Sultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2011-01-14 2019-04-27T00:41:45Z 2019-04-27T00:41:45Z 2019-05-20T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/873059 |
identifier_str_mv |
Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/873059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
510-519 |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503474560368640 |