Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.).
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017655 http://dx.doi.org/10.4238/2015.June.8.10 |
Resumo: | The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using random-amplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD primers. A total of 146 bands were generated, 141 of which were polymorphic (96.58%), with a variation of 4 and 12 loci and an average of 7 bands per primer. A dendrogram revealed a clear separation between the three populations (0.57). Data reliability and node consistency were verified by bootstrap values and the cophenetic correlation coefficient (88.15%). Coefficients of similarity between pairs of genotypes ranged from 0.26 to 0.86, with a mean of 0.57. Nei?s genetic diversity index (HE) value of the population sampled in Teresina was 0.212, of Esperantina it was 0.195, and of José de Freitas it was 0.207. After the HE was decomposed, the complete diversity was found to be 0.3213. Genetic differentiation between populations was0.362, and the estimation of gene flow between populations was low (0.879). Analysis of molecular variance revealed that 59.52% of the variation was contained within populations, and 40.48% was between populations. RAPD markers were effective for genetic diversity analysis within and between natural babassu populations, and exhibited a high level of polymorphism. Genetic diversity was the highest within populations; variability was lower in the managed populations than in the undisturbed populations. |
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Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.).Diversidade genéticaManejo populacionalCoeficiente de similaridadeGenetic diversityPalm treePopulation managementSimilarity coefficientsPalmeira oleaginosaBabaçuThe purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using random-amplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD primers. A total of 146 bands were generated, 141 of which were polymorphic (96.58%), with a variation of 4 and 12 loci and an average of 7 bands per primer. A dendrogram revealed a clear separation between the three populations (0.57). Data reliability and node consistency were verified by bootstrap values and the cophenetic correlation coefficient (88.15%). Coefficients of similarity between pairs of genotypes ranged from 0.26 to 0.86, with a mean of 0.57. Nei?s genetic diversity index (HE) value of the population sampled in Teresina was 0.212, of Esperantina it was 0.195, and of José de Freitas it was 0.207. After the HE was decomposed, the complete diversity was found to be 0.3213. Genetic differentiation between populations was0.362, and the estimation of gene flow between populations was low (0.879). Analysis of molecular variance revealed that 59.52% of the variation was contained within populations, and 40.48% was between populations. RAPD markers were effective for genetic diversity analysis within and between natural babassu populations, and exhibited a high level of polymorphism. Genetic diversity was the highest within populations; variability was lower in the managed populations than in the undisturbed populations.M. F. SANTOS, Universidade Federal do Piauí.; KAESEL JACKSON DAMASCENO E SILVA, CPAMN; MARIANA APARECIDA CARVALHAES, CPAMN; PAULO SARMANHO DA COSTA LIMA, CPAMN.SANTOS, M. F.SILVA, K. J. D. eCARVALHAES, M. A.LIMA, P. S. da C.2015-06-15T11:11:11Z2015-06-15T11:11:11Z2015-06-1520152015-06-15T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 14, n. 2, p. 6124-6135, June 2015.1678-4685http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017655http://dx.doi.org/10.4238/2015.June.8.10enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:22:34Zoai:www.alice.cnptia.embrapa.br:doc/1017655Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:22:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:22:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
title |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
spellingShingle |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). SANTOS, M. F. Diversidade genética Manejo populacional Coeficiente de similaridade Genetic diversity Palm tree Population management Similarity coefficients Palmeira oleaginosa Babaçu |
title_short |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
title_full |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
title_fullStr |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
title_full_unstemmed |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
title_sort |
Genetic variation detected by RAPD markers in natural populations of babassu palm (Attalea speciosa Mart.). |
author |
SANTOS, M. F. |
author_facet |
SANTOS, M. F. SILVA, K. J. D. e CARVALHAES, M. A. LIMA, P. S. da C. |
author_role |
author |
author2 |
SILVA, K. J. D. e CARVALHAES, M. A. LIMA, P. S. da C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
M. F. SANTOS, Universidade Federal do Piauí.; KAESEL JACKSON DAMASCENO E SILVA, CPAMN; MARIANA APARECIDA CARVALHAES, CPAMN; PAULO SARMANHO DA COSTA LIMA, CPAMN. |
dc.contributor.author.fl_str_mv |
SANTOS, M. F. SILVA, K. J. D. e CARVALHAES, M. A. LIMA, P. S. da C. |
dc.subject.por.fl_str_mv |
Diversidade genética Manejo populacional Coeficiente de similaridade Genetic diversity Palm tree Population management Similarity coefficients Palmeira oleaginosa Babaçu |
topic |
Diversidade genética Manejo populacional Coeficiente de similaridade Genetic diversity Palm tree Population management Similarity coefficients Palmeira oleaginosa Babaçu |
description |
The purpose of this study was to analyze the effects of management on the genetic structure of natural populations of Attalea speciosa in the State of Piauí, Brazil, using random-amplified polymorphic DNA (RAPD) markers. Three babassu populations under different management systems were selected. Polymerase chain reactions were performed for 20 RAPD primers. A total of 146 bands were generated, 141 of which were polymorphic (96.58%), with a variation of 4 and 12 loci and an average of 7 bands per primer. A dendrogram revealed a clear separation between the three populations (0.57). Data reliability and node consistency were verified by bootstrap values and the cophenetic correlation coefficient (88.15%). Coefficients of similarity between pairs of genotypes ranged from 0.26 to 0.86, with a mean of 0.57. Nei?s genetic diversity index (HE) value of the population sampled in Teresina was 0.212, of Esperantina it was 0.195, and of José de Freitas it was 0.207. After the HE was decomposed, the complete diversity was found to be 0.3213. Genetic differentiation between populations was0.362, and the estimation of gene flow between populations was low (0.879). Analysis of molecular variance revealed that 59.52% of the variation was contained within populations, and 40.48% was between populations. RAPD markers were effective for genetic diversity analysis within and between natural babassu populations, and exhibited a high level of polymorphism. Genetic diversity was the highest within populations; variability was lower in the managed populations than in the undisturbed populations. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-15T11:11:11Z 2015-06-15T11:11:11Z 2015-06-15 2015 2015-06-15T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 2, p. 6124-6135, June 2015. 1678-4685 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017655 http://dx.doi.org/10.4238/2015.June.8.10 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 2, p. 6124-6135, June 2015. 1678-4685 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017655 http://dx.doi.org/10.4238/2015.June.8.10 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503407743008768 |