Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).

Detalhes bibliográficos
Autor(a) principal: MALABARBA, J.
Data de Publicação: 2018
Outros Autores: BUFFON, V., MARIATH, J. E. A., MARASHIN, F. S., MARGIS-PINHEIRO, M., PASQUALI, G., REVERS, L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200
Resumo: Seedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11
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spelling Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).Plant plasmidGrapevineSeedSilencingOverexpressionVviAGL11Seedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, BraZIL; VANESSA BUFFON, CNPUV; Jorge E.A. Mariath, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Felipe S. Maraschin, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Márcia Margis-Pinheiro, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV.MALABARBA, J.BUFFON, V.MARIATH, J. E. A.MARASHIN, F. S.MARGIS-PINHEIRO, M.PASQUALI, G.REVERS, L. F.2019-01-07T23:54:59Z2019-01-07T23:54:59Z2019-01-0720182019-04-27T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Science, v. 269, p. 126-135, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-01-07T23:55:05Zoai:www.alice.cnptia.embrapa.br:doc/1103200Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-01-07T23:55:05falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-01-07T23:55:05Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
title Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
spellingShingle Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
MALABARBA, J.
Plant plasmid
Grapevine
Seed
Silencing
Overexpression
VviAGL11
title_short Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
title_full Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
title_fullStr Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
title_full_unstemmed Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
title_sort Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.).
author MALABARBA, J.
author_facet MALABARBA, J.
BUFFON, V.
MARIATH, J. E. A.
MARASHIN, F. S.
MARGIS-PINHEIRO, M.
PASQUALI, G.
REVERS, L. F.
author_role author
author2 BUFFON, V.
MARIATH, J. E. A.
MARASHIN, F. S.
MARGIS-PINHEIRO, M.
PASQUALI, G.
REVERS, L. F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, BraZIL; VANESSA BUFFON, CNPUV; Jorge E.A. Mariath, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Felipe S. Maraschin, raduate Program in Botany, Botany Department, Institute for Bioscience, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Márcia Margis-Pinheiro, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV.
dc.contributor.author.fl_str_mv MALABARBA, J.
BUFFON, V.
MARIATH, J. E. A.
MARASHIN, F. S.
MARGIS-PINHEIRO, M.
PASQUALI, G.
REVERS, L. F.
dc.subject.por.fl_str_mv Plant plasmid
Grapevine
Seed
Silencing
Overexpression
VviAGL11
topic Plant plasmid
Grapevine
Seed
Silencing
Overexpression
VviAGL11
description Seedlessness in grapes is a desirable trait, especially for in natura consumption. Previously, we showed that VviAGL11 is the main responsible gene for seed morphogenesis in grapevine. Here we tested the function of this gene in grapevine with the use of plant plasmids. VviAGL11 was cloned into silencing and overexpression versions of p28iIR plasmid. Reproductive grapevine bunches from di ff erent seeded and seedless cultivars were separately treated with VviAGL11 -harboring plasmids, along with controls. Plasmids were detected in leaves after a month of treatment, and berries, leaves, stems and seeds were analyzed for ectopic gene expression by RT- qPCR after 90 days of plasmid injection. Fruits from the seedless ? Linda ? treated with the VviAGL11-over- expression plasmid showed high expression levels of VviAGL11 and exhibited small seeds that were not found in the untreated control samples. Mature grapes from seeded ? Italia ? and ? Ruby ? bunches treated with the VviAGL11-silencing plasmid showed decreased VviAGL11 expression, reduced number of seeds and increased number of seed traces. The present study con fi rms that VviAGL11 is a key master regulator of seed morpho- genesis in grapevine and corroborates with the applicability of plant plasmids as promising biotechnological tools to functionally test genes in perennial plants in a rapid and confident way. Keywords: Plant plasmid Grapevine Seed Silencing Overexpression VviAGL11
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-01-07T23:54:59Z
2019-01-07T23:54:59Z
2019-01-07
2019-04-27T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plant Science, v. 269, p. 126-135, 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200
identifier_str_mv Plant Science, v. 269, p. 126-135, 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103200
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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