Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150143 https://doi.org/10.3390/genes13050769 |
Resumo: | Abstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain. |
id |
EMBR_d14734d472a409aa23b2d2c44065c145 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1150143 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts.MarrãsEstresse agudoExpressão gênicaAngiogêneseAcute stressLPSRT-qPCRReprodução AnimalMarraAngiogenesisAnimal developmentSwineGiltsAbstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain.ARTHUR NERY DA SILVA, Universidade de São Paulo; LUANA ALVES, Universidade de São Paulo; GERMANA VIZZOTTO OSOWSKI, Universidade de São Paulo; LEANDRO SABEI, Universidade de São Paulo; PRISCILA ASSIS FERRAZ, Universidade de São Paulo; GUILHERME PUGLIESI, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; RICARDO ZANELLA, Universidade de Passo Fundo; ADROALDO JOSÉ ZANELLA, Universidade de São Paulo.SILVA, A. N. daALVES, L.OSOWSKI, G. V.SABEI, L.FERRAZ, P. A.PUGLIESI, G.MARQUES, M. G.ZANELLA, R.ZANELLA, A. J.2022-12-21T13:01:45Z2022-12-21T13:01:45Z2022-12-212022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenes, v. 13, n. 5, p. 1-13, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150143https://doi.org/10.3390/genes13050769enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-12-21T13:01:45Zoai:www.alice.cnptia.embrapa.br:doc/1150143Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-12-21T13:01:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-12-21T13:01:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
title |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
spellingShingle |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. SILVA, A. N. da Marrãs Estresse agudo Expressão gênica Angiogênese Acute stress LPS RT-qPCR Reprodução Animal Marra Angiogenesis Animal development Swine Gilts |
title_short |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
title_full |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
title_fullStr |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
title_full_unstemmed |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
title_sort |
Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. |
author |
SILVA, A. N. da |
author_facet |
SILVA, A. N. da ALVES, L. OSOWSKI, G. V. SABEI, L. FERRAZ, P. A. PUGLIESI, G. MARQUES, M. G. ZANELLA, R. ZANELLA, A. J. |
author_role |
author |
author2 |
ALVES, L. OSOWSKI, G. V. SABEI, L. FERRAZ, P. A. PUGLIESI, G. MARQUES, M. G. ZANELLA, R. ZANELLA, A. J. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARTHUR NERY DA SILVA, Universidade de São Paulo; LUANA ALVES, Universidade de São Paulo; GERMANA VIZZOTTO OSOWSKI, Universidade de São Paulo; LEANDRO SABEI, Universidade de São Paulo; PRISCILA ASSIS FERRAZ, Universidade de São Paulo; GUILHERME PUGLIESI, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; RICARDO ZANELLA, Universidade de Passo Fundo; ADROALDO JOSÉ ZANELLA, Universidade de São Paulo. |
dc.contributor.author.fl_str_mv |
SILVA, A. N. da ALVES, L. OSOWSKI, G. V. SABEI, L. FERRAZ, P. A. PUGLIESI, G. MARQUES, M. G. ZANELLA, R. ZANELLA, A. J. |
dc.subject.por.fl_str_mv |
Marrãs Estresse agudo Expressão gênica Angiogênese Acute stress LPS RT-qPCR Reprodução Animal Marra Angiogenesis Animal development Swine Gilts |
topic |
Marrãs Estresse agudo Expressão gênica Angiogênese Acute stress LPS RT-qPCR Reprodução Animal Marra Angiogenesis Animal development Swine Gilts |
description |
Abstract: The corpus luteum (CL) is a temporary endocrine gland that plays a decisive role in the reproductive physiology of gilts. Recently, it has been suggested that exogenous factors may compromise the normal functioning of the CL. In the present study, we aimed to understand to what extent an acute and systemic challenge with lipopolysaccharide (LPS) on the day of estrus could compromise gene expression of gilts' CLs housed in different welfare conditions. For this, we housed 42 gilts in three different housing systems: crates, indoor group pens, and outdoor housing. Then, we challenged six females from each group with LPS and eight with saline (SAL) on the day of estrus. After slaughtering the gilts on the fifth day after the challenge, ovaries were collected for gene expression analysis by RT-qPCR. Housing system and LPS challenge did not have a significant interaction for any genes evaluated; thus, their effects were studied separately. We identified significant (p < 0.05) downregulation of the angiogenic genes VEGF and FTL1 among LPS-challenged animals. Meanwhile, we also observed upregulation of HSD3B1 gene among LPS-challenged animals. We found that STAR and LHCGR genes were differentially expressed depending on the housing system, which indicates that the environment may affect adaptation capabilities. Our results indicate that an acute health challenge on the estrus day alters CL gene expression; however, the role of the housing system remains uncertain. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-21T13:01:45Z 2022-12-21T13:01:45Z 2022-12-21 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genes, v. 13, n. 5, p. 1-13, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150143 https://doi.org/10.3390/genes13050769 |
identifier_str_mv |
Genes, v. 13, n. 5, p. 1-13, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150143 https://doi.org/10.3390/genes13050769 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503536429498368 |