Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.

Detalhes bibliográficos
Autor(a) principal: OLIVEIRA, E. R.
Data de Publicação: 2021
Outros Autores: FAYER, L., ZANETTE, R. S. S., LADEIRA, L. O., OLIVEIRA, L. F. C. de, MARANDUBA, C. M. C., BRANDAO, H. de M., MUNK, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135728
https://doi.org/10.1088/1361-6528/ac335b
Resumo: Carboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNTCOOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields.
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spelling Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.NanotecnologiaEstudo in vitroEngenharia de tecidoCélulas-troncoCarbonoCarboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNTCOOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields.EDUARDA R. OLIVEIRA, Universidade Federal de Juiz de Fora; LEONARA FAYER, Universidade Federal de Juiz de Fora; RAFAELLA S. S. ZANETTE, Universidade Federal de Juiz de Fora; LUIZ O. LADEIRA, Universidade Federal de Minas Gerais; LUIZ F. C. DE OLIVEIRA, Universidade Federal de Juiz de Fora; CARLOS M. C. MARANDUBA, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora.OLIVEIRA, E. R.FAYER, L.ZANETTE, R. S. S.LADEIRA, L. O.OLIVEIRA, L. F. C. deMARANDUBA, C. M. C.BRANDAO, H. de M.MUNK, M.2023-10-04T10:23:55Z2023-10-04T10:23:55Z2021-10-302022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNanotechnology, v. 33, n. 6, 065101, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135728https://doi.org/10.1088/1361-6528/ac335benginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-10-04T10:23:55Zoai:www.alice.cnptia.embrapa.br:doc/1135728Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-10-04T10:23:55falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-10-04T10:23:55Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
title Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
spellingShingle Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
OLIVEIRA, E. R.
Nanotecnologia
Estudo in vitro
Engenharia de tecido
Células-tronco
Carbono
title_short Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
title_full Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
title_fullStr Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
title_full_unstemmed Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
title_sort Cytocompatibility of carboxylated multi-wall carbon nanotubes in stem cells from human exfoliated deciduous teeth.
author OLIVEIRA, E. R.
author_facet OLIVEIRA, E. R.
FAYER, L.
ZANETTE, R. S. S.
LADEIRA, L. O.
OLIVEIRA, L. F. C. de
MARANDUBA, C. M. C.
BRANDAO, H. de M.
MUNK, M.
author_role author
author2 FAYER, L.
ZANETTE, R. S. S.
LADEIRA, L. O.
OLIVEIRA, L. F. C. de
MARANDUBA, C. M. C.
BRANDAO, H. de M.
MUNK, M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv EDUARDA R. OLIVEIRA, Universidade Federal de Juiz de Fora; LEONARA FAYER, Universidade Federal de Juiz de Fora; RAFAELLA S. S. ZANETTE, Universidade Federal de Juiz de Fora; LUIZ O. LADEIRA, Universidade Federal de Minas Gerais; LUIZ F. C. DE OLIVEIRA, Universidade Federal de Juiz de Fora; CARLOS M. C. MARANDUBA, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora.
dc.contributor.author.fl_str_mv OLIVEIRA, E. R.
FAYER, L.
ZANETTE, R. S. S.
LADEIRA, L. O.
OLIVEIRA, L. F. C. de
MARANDUBA, C. M. C.
BRANDAO, H. de M.
MUNK, M.
dc.subject.por.fl_str_mv Nanotecnologia
Estudo in vitro
Engenharia de tecido
Células-tronco
Carbono
topic Nanotecnologia
Estudo in vitro
Engenharia de tecido
Células-tronco
Carbono
description Carboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained viability after MWCNT-COOH exposure. MWCNTCOOH did not alter the superoxide dismutase activity and did not cause genotoxic effects. The present findings are relevant to the potential application of MWCNT-COOH in the tissue engineering and regenerative medicine fields.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-30
2022
2023-10-04T10:23:55Z
2023-10-04T10:23:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Nanotechnology, v. 33, n. 6, 065101, 2022.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135728
https://doi.org/10.1088/1361-6528/ac335b
identifier_str_mv Nanotechnology, v. 33, n. 6, 065101, 2022.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135728
https://doi.org/10.1088/1361-6528/ac335b
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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