The hypoosmotic swelling test in fresh goat spermatozoa.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/663713 |
Resumo: | The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa. |
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The hypoosmotic swelling test in fresh goat spermatozoa.GoatHypoosmotic swelling testSpermCaprinoReprodução animalSêmenCongelamentoAnimal reproductionTailThe hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa.JEFERSON FERREIRA DA FONSECA, CNPC; UFV, Viçosa, MG; UFV; UFV; UFV; UFV; UFV.FONSECA, J. F. daTORRES, C. A. A.MAFFILI, V. V.BORGES, A. M.SANTOS, A. D. F.RODRIGUES, M. T.OLIVEIRA, R. F. M.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2010-04-0920052019-09-25T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction, v. 2, n. 2, p. 139-144, Apr./Jun. 2005.http://www.alice.cnptia.embrapa.br/alice/handle/doc/663713enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:12:50Zoai:www.alice.cnptia.embrapa.br:doc/663713Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:12:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:12:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
The hypoosmotic swelling test in fresh goat spermatozoa. |
title |
The hypoosmotic swelling test in fresh goat spermatozoa. |
spellingShingle |
The hypoosmotic swelling test in fresh goat spermatozoa. FONSECA, J. F. da Goat Hypoosmotic swelling test Sperm Caprino Reprodução animal Sêmen Congelamento Animal reproduction Tail |
title_short |
The hypoosmotic swelling test in fresh goat spermatozoa. |
title_full |
The hypoosmotic swelling test in fresh goat spermatozoa. |
title_fullStr |
The hypoosmotic swelling test in fresh goat spermatozoa. |
title_full_unstemmed |
The hypoosmotic swelling test in fresh goat spermatozoa. |
title_sort |
The hypoosmotic swelling test in fresh goat spermatozoa. |
author |
FONSECA, J. F. da |
author_facet |
FONSECA, J. F. da TORRES, C. A. A. MAFFILI, V. V. BORGES, A. M. SANTOS, A. D. F. RODRIGUES, M. T. OLIVEIRA, R. F. M. |
author_role |
author |
author2 |
TORRES, C. A. A. MAFFILI, V. V. BORGES, A. M. SANTOS, A. D. F. RODRIGUES, M. T. OLIVEIRA, R. F. M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
JEFERSON FERREIRA DA FONSECA, CNPC; UFV, Viçosa, MG; UFV; UFV; UFV; UFV; UFV. |
dc.contributor.author.fl_str_mv |
FONSECA, J. F. da TORRES, C. A. A. MAFFILI, V. V. BORGES, A. M. SANTOS, A. D. F. RODRIGUES, M. T. OLIVEIRA, R. F. M. |
dc.subject.por.fl_str_mv |
Goat Hypoosmotic swelling test Sperm Caprino Reprodução animal Sêmen Congelamento Animal reproduction Tail |
topic |
Goat Hypoosmotic swelling test Sperm Caprino Reprodução animal Sêmen Congelamento Animal reproduction Tail |
description |
The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including cattle, horses, and swine. However, the best approach for using this technique in goat semen has not been tested. The present study aimed to establish the best hypoosmotic solution (HS) for testing membrane integrity in fresh goat semen. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/l) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/l (S10). Twenty-eight semen samples were collected from seven mature bucks (four collections per buck) at 48 hour intervals. After macroscopic evaluation, 10?l of semen was immediately added to 2ml of each solution and incubated for one hour at 37ºC. Sequentially, 20?l of semen diluted in HS were evaluated with oil immersion using a phase-contrast microscope. A total of 200 spermatozoa were counted in at least five different fields, and sperm tails were classified as non-coiled, coiled, and strongly coiled. The respective percentages of spermatozoa with coiled tails (coiled plus strongly coiled) in the ten solutions listed above were 34.1, 38.8, 45.3, 51.5, 46.8, 42.8, 38.2, 29.0, 19.4 and 23.1%. Percentages of strongly coiled spermatozoa were: 6.8, 10.6, 21.5, 25.3, 24.3, 21.5, 19.3, 12.4, 6.4, and 7.9 for the ten solutions, respectively. According to total coiling, S4 was superior to S1, S7, S8, S9, and S10 (P<0.05). According to strong coiling, S4 was superior to S1, S2, S8, S9, and S10 (P<0.05). Results suggest that the 125 mOsm/l solution would be best for use in HOST in fresh goat spermatozoa. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2010-04-09 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2019-09-25T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Animal Reproduction, v. 2, n. 2, p. 139-144, Apr./Jun. 2005. http://www.alice.cnptia.embrapa.br/alice/handle/doc/663713 |
identifier_str_mv |
Animal Reproduction, v. 2, n. 2, p. 139-144, Apr./Jun. 2005. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/663713 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503375240298496 |