CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN

Detalhes bibliográficos
Autor(a) principal: Oliveira, Isabelle R. Sousa de
Data de Publicação: 2013
Outros Autores: Alves, Heron Medeiros, Castelo, Thibério Souza, Bezerra, Francisco Silvestre B., Bezerra, Ana Carla D. S., Silva, Alexandre Rodrigues
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/12921
Resumo: We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm.
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spelling CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMENCORRELAÇÕES ENTRE O TESTE HIPOSMÓTICO E A AVALIAÇÃO CLÁSSICA DO SÊMEN DE CAPRINOSAnimal reproductionCaprinoCorrelaçãoSêmenTeste Hiposmótico.We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm.Avaliou-se a correlação entre a avaliação da integridade funcional de membrana espermática por meio do teste hiposmótico, utilizando-se soluções com diferentes osmolaridades, e as avaliações convencionais do sêmen fresco de caprinos. Foram utilizados 24 ejaculados de três caprinos machos, obtidos por vagina artificial, os quais foram imediatamente submetidos à avaliação clássica. Em seguida, as amostras foram divididas em cinco alíquotas e submetidas ao teste hiposmótico, utilizando-se água destilada (0 mOsm/L) e soluções de citrato de sódio e frutose de diferentes osmolaridades (50; 100; 150 e 200 mOsm/L). A solução de 100 mOsm/L obteve a maior média de espermatozoides reagidos (34,8%) ao teste hiposmótico (P < 0,05); por outro lado, a água destilada foi a que apresentou os menores valores (20,8%). Não foram detectadas correlações significativas entre os espermatozoides reagidos ao teste hiposmótico e as demais características seminais (P > 0,05). Apesar disso, recomenda-se que o teste hiposmótico seja realizado utilizando-se uma solução a base de citrato de sódio e frutose apresentando 100 mOsm/L para a avaliação da integridade funcional da membrana espermática na espécie caprina.PALAVRAS-CHAVE: caprino; espermatozoide; teste hiposmótico.Universidade Federal de Goiás2013-06-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa científicaapplication/pdftext/htmlapplication/pdfhttps://revistas.ufg.br/vet/article/view/1292110.5216/cab.v14i2.12921Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 2 (2013); 216-221Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 2 (2013); 216-2211809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/12921/14538https://revistas.ufg.br/vet/article/view/12921/14539https://revistas.ufg.br/vet/article/view/12921/15447Oliveira, Isabelle R. Sousa deAlves, Heron MedeirosCastelo, Thibério SouzaBezerra, Francisco Silvestre B.Bezerra, Ana Carla D. S.Silva, Alexandre Rodriguesinfo:eu-repo/semantics/openAccess2013-06-28T13:39:57Zoai:ojs.revistas.ufg.br:article/12921Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2013-06-28T13:39:57Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)false
dc.title.none.fl_str_mv CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
CORRELAÇÕES ENTRE O TESTE HIPOSMÓTICO E A AVALIAÇÃO CLÁSSICA DO SÊMEN DE CAPRINOS
title CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
spellingShingle CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
Oliveira, Isabelle R. Sousa de
Animal reproduction
Caprino
Correlação
Sêmen
Teste Hiposmótico.
title_short CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
title_full CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
title_fullStr CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
title_full_unstemmed CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
title_sort CORRELATIONS BETWEEN HYPOOSMOTIC SWELLING TEST AND THE CLASSICAL EVALUATION OF GOAT SEMEN
author Oliveira, Isabelle R. Sousa de
author_facet Oliveira, Isabelle R. Sousa de
Alves, Heron Medeiros
Castelo, Thibério Souza
Bezerra, Francisco Silvestre B.
Bezerra, Ana Carla D. S.
Silva, Alexandre Rodrigues
author_role author
author2 Alves, Heron Medeiros
Castelo, Thibério Souza
Bezerra, Francisco Silvestre B.
Bezerra, Ana Carla D. S.
Silva, Alexandre Rodrigues
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Isabelle R. Sousa de
Alves, Heron Medeiros
Castelo, Thibério Souza
Bezerra, Francisco Silvestre B.
Bezerra, Ana Carla D. S.
Silva, Alexandre Rodrigues
dc.subject.por.fl_str_mv Animal reproduction
Caprino
Correlação
Sêmen
Teste Hiposmótico.
topic Animal reproduction
Caprino
Correlação
Sêmen
Teste Hiposmótico.
description We evaluated the correlation between the assessment of the functional integrity of the sperm membrane by hypoosmotic swelling tes,t using solutions with different osmolarities, and the conventional assessments of fresh semen in goats. A total of 24 ejaculates from three goats was obtained by artificial vagina and immediately submitted to the classical evaluation. Samples were divided into five aliquots and subjected to hypoosmotic test using distilled water (0 mOsm/L), and sodium citrate and fructose solutions at different osmolarities (50; 100; 150 and 200 mOsm/L). The 100 mOsm/L solution showed the highest percentage of reacted sperm (34.8%), but distilled water was the one with the lowest values (20.8%). No significant correlations were detected between the reacted sperm verified by the hypoosmotic swelling test and other semen characteristics (P> 0.05). Nevertheless, we recommend the carry out of the hypoosmotic test by using a 100 mOsm/L sodium citrate and fructose solution to assess the functional integrity of the sperm membrane in caprine species.KEYWORDS: caprine; hyposmotic swelling test; sperm.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/12921
10.5216/cab.v14i2.12921
url https://revistas.ufg.br/vet/article/view/12921
identifier_str_mv 10.5216/cab.v14i2.12921
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/12921/14538
https://revistas.ufg.br/vet/article/view/12921/14539
https://revistas.ufg.br/vet/article/view/12921/15447
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 14 No. 2 (2013); 216-221
Ciência Animal Brasileira / Brazilian Animal Science; v. 14 n. 2 (2013); 216-221
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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