Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

Detalhes bibliográficos
Autor(a) principal: Oliveira,GH
Data de Publicação: 2006
Outros Autores: Berchieri Jr,A, Montassier,HJ
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008
Resumo: This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
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spelling Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by ElisaChickenELISAenzymeSalmonella spserologyThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.Fundacao de Apoio a Ciência e Tecnologia Avicolas2006-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008Brazilian Journal of Poultry Science v.8 n.1 2006reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2006000100008info:eu-repo/semantics/openAccessOliveira,GHBerchieri Jr,AMontassier,HJeng2006-06-06T00:00:00Zoai:scielo:S1516-635X2006000100008Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2006-06-06T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
spellingShingle Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Oliveira,GH
Chicken
ELISA
enzyme
Salmonella sp
serology
title_short Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_full Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_fullStr Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_full_unstemmed Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_sort Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
author Oliveira,GH
author_facet Oliveira,GH
Berchieri Jr,A
Montassier,HJ
author_role author
author2 Berchieri Jr,A
Montassier,HJ
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,GH
Berchieri Jr,A
Montassier,HJ
dc.subject.por.fl_str_mv Chicken
ELISA
enzyme
Salmonella sp
serology
topic Chicken
ELISA
enzyme
Salmonella sp
serology
description This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
publishDate 2006
dc.date.none.fl_str_mv 2006-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-635X2006000100008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.8 n.1 2006
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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