Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
Resumo: | This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis. |
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Brazilian Journal of Poultry Science (Online) |
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Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by ElisaChickensconjugateELISASalmonella spserologyThis study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.Fundacao de Apoio a Ciência e Tecnologia Avicolas2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007Brazilian Journal of Poultry Science v.6 n.2 2004reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2004000200007info:eu-repo/semantics/openAccessOliveira,GH de<A NAME="nt01a"></A>Berchieri Júnior,ÂMontassier,HJFernandes,ACeng2004-10-18T00:00:00Zoai:scielo:S1516-635X2004000200007Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2004-10-18T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
title |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
spellingShingle |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa Oliveira,GH de<A NAME="nt01a"></A> Chickens conjugate ELISA Salmonella sp serology |
title_short |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
title_full |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
title_fullStr |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
title_full_unstemmed |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
title_sort |
Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa |
author |
Oliveira,GH de<A NAME="nt01a"></A> |
author_facet |
Oliveira,GH de<A NAME="nt01a"></A> Berchieri Júnior, Montassier,HJ Fernandes,AC |
author_role |
author |
author2 |
Berchieri Júnior, Montassier,HJ Fernandes,AC |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira,GH de<A NAME="nt01a"></A> Berchieri Júnior, Montassier,HJ Fernandes,AC |
dc.subject.por.fl_str_mv |
Chickens conjugate ELISA Salmonella sp serology |
topic |
Chickens conjugate ELISA Salmonella sp serology |
description |
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-635X2004000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.6 n.2 2004 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122510891220992 |