Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700003 |
Resumo: | The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. |
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Memórias do Instituto Oswaldo Cruz |
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Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazilcalicivirusacute gastroenteritisreverse transcriptasepolymerase chain reactionBrazilThe objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.Instituto Oswaldo Cruz, Ministério da Saúde2006-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700003Memórias do Instituto Oswaldo Cruz v.101 n.7 2006reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762006000700003info:eu-repo/semantics/openAccessBorges,Ana Maria TavaresTeixeira,José Marcus SócratesCosta,Paulo Sérgio Sucasas daGiugliano,Loreny GimenesFiaccadori,Fabíola SouzaFranco,Rachel de Carvalho eBrito,Wilia Marta Elsner Diederichsen deLeite,José Paulo GagliardiCardoso,Divina das Dores de Paulaeng2020-04-25T17:49:38Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:13:59.578Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
spellingShingle |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil Borges,Ana Maria Tavares calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
title_short |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_full |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_fullStr |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_full_unstemmed |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_sort |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
author |
Borges,Ana Maria Tavares |
author_facet |
Borges,Ana Maria Tavares Teixeira,José Marcus Sócrates Costa,Paulo Sérgio Sucasas da Giugliano,Loreny Gimenes Fiaccadori,Fabíola Souza Franco,Rachel de Carvalho e Brito,Wilia Marta Elsner Diederichsen de Leite,José Paulo Gagliardi Cardoso,Divina das Dores de Paula |
author_role |
author |
author2 |
Teixeira,José Marcus Sócrates Costa,Paulo Sérgio Sucasas da Giugliano,Loreny Gimenes Fiaccadori,Fabíola Souza Franco,Rachel de Carvalho e Brito,Wilia Marta Elsner Diederichsen de Leite,José Paulo Gagliardi Cardoso,Divina das Dores de Paula |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Borges,Ana Maria Tavares Teixeira,José Marcus Sócrates Costa,Paulo Sérgio Sucasas da Giugliano,Loreny Gimenes Fiaccadori,Fabíola Souza Franco,Rachel de Carvalho e Brito,Wilia Marta Elsner Diederichsen de Leite,José Paulo Gagliardi Cardoso,Divina das Dores de Paula |
dc.subject.por.fl_str_mv |
calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
topic |
calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
dc.description.none.fl_txt_mv |
The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. |
description |
The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700003 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762006000700003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.101 n.7 2006 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937697121107968 |