Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/26609 https://dx.doi.org/10.1590/S0074-02762006000700003 |
Resumo: | The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. |
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Borges, Ana Maria TavaresTeixeira, José Marcus SócratesCosta, Paulo Sérgio Sucasas daGiugliano, Loreny GimenesFiaccadori, Fabíola SouzaFranco, Rachel de Carvalho eBrito, Wilia Marta Elsner Diederichsen deLeite, José Paulo GagliardiCardoso, Divina das Dores de Paula2017-12-07T04:44:14Z2017-12-07T04:44:14Z2006Mem. Inst. Oswaldo Cruz,v.101,n.7,p.721-724,2006http://repositorio.unb.br/handle/10482/26609https://dx.doi.org/10.1590/S0074-02762006000700003The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.Em processamentoInstituto Oswaldo Cruz, Ministério da SaúdeDetection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlecalicivirusacute gastroenteritisreverse transcriptasepolymerase chain reactionBrazilinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALv101n7a03.pdfapplication/pdf149698http://repositorio2.unb.br/jspui/bitstream/10482/26609/1/v101n7a03.pdf402be9008f7d879d342145b1945301f6MD51open access10482/266092023-09-29 14:01:49.048open accessoai:repositorio2.unb.br:10482/26609Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-09-29T17:01:49Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.pt_BR.fl_str_mv |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
spellingShingle |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil Borges, Ana Maria Tavares calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
title_short |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_full |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_fullStr |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_full_unstemmed |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
title_sort |
Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil |
author |
Borges, Ana Maria Tavares |
author_facet |
Borges, Ana Maria Tavares Teixeira, José Marcus Sócrates Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Fiaccadori, Fabíola Souza Franco, Rachel de Carvalho e Brito, Wilia Marta Elsner Diederichsen de Leite, José Paulo Gagliardi Cardoso, Divina das Dores de Paula |
author_role |
author |
author2 |
Teixeira, José Marcus Sócrates Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Fiaccadori, Fabíola Souza Franco, Rachel de Carvalho e Brito, Wilia Marta Elsner Diederichsen de Leite, José Paulo Gagliardi Cardoso, Divina das Dores de Paula |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Borges, Ana Maria Tavares Teixeira, José Marcus Sócrates Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Fiaccadori, Fabíola Souza Franco, Rachel de Carvalho e Brito, Wilia Marta Elsner Diederichsen de Leite, José Paulo Gagliardi Cardoso, Divina das Dores de Paula |
dc.subject.keyword.pt_BR.fl_str_mv |
calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
topic |
calicivirus acute gastroenteritis reverse transcriptase polymerase chain reaction Brazil |
description |
The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2017-12-07T04:44:14Z |
dc.date.available.fl_str_mv |
2017-12-07T04:44:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Mem. Inst. Oswaldo Cruz,v.101,n.7,p.721-724,2006 |
dc.identifier.uri.fl_str_mv |
http://repositorio.unb.br/handle/10482/26609 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://dx.doi.org/10.1590/S0074-02762006000700003 |
identifier_str_mv |
Mem. Inst. Oswaldo Cruz,v.101,n.7,p.721-724,2006 |
url |
http://repositorio.unb.br/handle/10482/26609 https://dx.doi.org/10.1590/S0074-02762006000700003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
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Universidade de Brasília (UnB) |
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UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
bitstream.url.fl_str_mv |
http://repositorio2.unb.br/jspui/bitstream/10482/26609/1/v101n7a03.pdf |
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402be9008f7d879d342145b1945301f6 |
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Repositório Institucional da UnB - Universidade de Brasília (UnB) |
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