Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells

Detalhes bibliográficos
Autor(a) principal: Fonseca,CT
Data de Publicação: 2004
Outros Autores: Cunha-Neto,E, Kalil,J, Jesus,AR de, Correa-Oliveira,R, Carvalho,EM, Oliveira,SC
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011
Resumo: Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
id FIOCRUZ-4_d976602696327a788f15f10c4b4d2e9c
oai_identifier_str oai:scielo:S0074-02762004000900011
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cellsT cellssynthetic peptidesvaccinesSchistosoma mansoniSm14paramyosinParamyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.Instituto Oswaldo Cruz, Ministério da Saúde2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011Memórias do Instituto Oswaldo Cruz v.99 suppl.1 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000900011info:eu-repo/semantics/openAccessFonseca,CTCunha-Neto,EKalil,JJesus,AR deCorrea-Oliveira,RCarvalho,EMOliveira,SCeng2020-04-25T17:49:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:56.841Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
spellingShingle Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
Fonseca,CT
T cells
synthetic peptides
vaccines
Schistosoma mansoni
Sm14
paramyosin
title_short Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_full Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_fullStr Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_full_unstemmed Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_sort Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
author Fonseca,CT
author_facet Fonseca,CT
Cunha-Neto,E
Kalil,J
Jesus,AR de
Correa-Oliveira,R
Carvalho,EM
Oliveira,SC
author_role author
author2 Cunha-Neto,E
Kalil,J
Jesus,AR de
Correa-Oliveira,R
Carvalho,EM
Oliveira,SC
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca,CT
Cunha-Neto,E
Kalil,J
Jesus,AR de
Correa-Oliveira,R
Carvalho,EM
Oliveira,SC
dc.subject.por.fl_str_mv T cells
synthetic peptides
vaccines
Schistosoma mansoni
Sm14
paramyosin
topic T cells
synthetic peptides
vaccines
Schistosoma mansoni
Sm14
paramyosin
dc.description.none.fl_txt_mv Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
description Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000900011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 suppl.1 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937693111353344