Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011 |
Resumo: | Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity. |
id |
FIOCRUZ-4_d976602696327a788f15f10c4b4d2e9c |
---|---|
oai_identifier_str |
oai:scielo:S0074-02762004000900011 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cellsT cellssynthetic peptidesvaccinesSchistosoma mansoniSm14paramyosinParamyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.Instituto Oswaldo Cruz, Ministério da Saúde2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011Memórias do Instituto Oswaldo Cruz v.99 suppl.1 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000900011info:eu-repo/semantics/openAccessFonseca,CTCunha-Neto,EKalil,JJesus,AR deCorrea-Oliveira,RCarvalho,EMOliveira,SCeng2020-04-25T17:49:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:56.841Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
spellingShingle |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells Fonseca,CT T cells synthetic peptides vaccines Schistosoma mansoni Sm14 paramyosin |
title_short |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_full |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_fullStr |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_full_unstemmed |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_sort |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
author |
Fonseca,CT |
author_facet |
Fonseca,CT Cunha-Neto,E Kalil,J Jesus,AR de Correa-Oliveira,R Carvalho,EM Oliveira,SC |
author_role |
author |
author2 |
Cunha-Neto,E Kalil,J Jesus,AR de Correa-Oliveira,R Carvalho,EM Oliveira,SC |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca,CT Cunha-Neto,E Kalil,J Jesus,AR de Correa-Oliveira,R Carvalho,EM Oliveira,SC |
dc.subject.por.fl_str_mv |
T cells synthetic peptides vaccines Schistosoma mansoni Sm14 paramyosin |
topic |
T cells synthetic peptides vaccines Schistosoma mansoni Sm14 paramyosin |
dc.description.none.fl_txt_mv |
Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity. |
description |
Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762004000900011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.99 suppl.1 2004 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937693111353344 |