Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/6607 |
Resumo: | Trabalho completo: acesso restrito, p. 204–212 |
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Fonseca, Cristina ToscanoCunha Neto, EdécioGoldberg, Anna Carla Renata KrepelKalil, JorgeJesus, Amélia Maria Ribeiro deCarvalho Filho, Edgar Marcelino deOliveira, Rodrigo CorreaOliveira, Sérgio CostaFonseca, Cristina ToscanoCunha Neto, EdécioGoldberg, Anna Carla Renata KrepelKalil, JorgeJesus, Amélia Maria Ribeiro deCarvalho Filho, Edgar Marcelino deOliveira, Rodrigo CorreaOliveira, Sérgio Costa2012-08-20T19:09:29Z2005-021286-4579http://www.repositorio.ufba.br/ri/handle/ri/6607v. 7, n. 2Trabalho completo: acesso restrito, p. 204–212he development of a defined anti-schistosomiasis vaccine would contribute to the current control strategy mainly because immunization provides long-lasting immunity to the disease. Sm14, one of the six Schistosoma mansoni antigens selected by WHO as a candidate to compose a subunit vaccine against schistosomiasis, has been associated with resistance to S. mansoni infection in human beings and is able to induce protection in the murine model. To identify human T cell epitopes in Sm14, we used the TEPITOPE algorithm to select peptides that would most likely bind to several HLA-DR molecules. In this study, three Sm14 epitopes were selected and produced as synthetic peptides. Human T cell responses from schistosomiasis patients living in endemic areas in Brazil were determined by proliferation assay and IL-5 and IFN-γ measurements. Differential peptide recognition and cytokine production in response to Sm14 epitopes were observed in individuals resistant to S. mansoni infection versus susceptible individuals. Sm14(32-48) and Sm14(53-69) peptides were preferentially recognized by peripheral blood mononuclear cells (PBMCs) of S. mansoni-resistant individuals, and Sm14(53-69) induced significant production of IFN-γ. Additionally, Sm14(32-48) and Sm14(53-69) were “promiscuous” peptides, since they were able to induce cellular immune responses in individuals carrying 10 and 8, respectively, of the 11 HLA-DR molecules expressed in the studied population. Among Sm14 synthetic peptides tested in this study, we identified Sm14(32-48) and Sm14(53-69) as promising candidates to compose an anti-schistosomiasis vaccine, since they seem to be related to resistance to human schistosomiasis.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-08-20T19:09:29Z No. of bitstreams: 1 (172).pdf: 354283 bytes, checksum: 782806db1c3215c834afd72dc34157d5 (MD5)Made available in DSpace on 2012-08-20T19:09:29Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| dc.title.alternative.pt_BR.fl_str_mv |
Microbes and Infection |
| title |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| spellingShingle |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis Fonseca, Cristina Toscano Vaccine Human T cells Synthetic peptides Schistosoma mansoni Sm14 |
| title_short |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| title_full |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| title_fullStr |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| title_full_unstemmed |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| title_sort |
Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis |
| author |
Fonseca, Cristina Toscano |
| author_facet |
Fonseca, Cristina Toscano Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa |
| author_role |
author |
| author2 |
Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fonseca, Cristina Toscano Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa Fonseca, Cristina Toscano Cunha Neto, Edécio Goldberg, Anna Carla Renata Krepel Kalil, Jorge Jesus, Amélia Maria Ribeiro de Carvalho Filho, Edgar Marcelino de Oliveira, Rodrigo Correa Oliveira, Sérgio Costa |
| dc.subject.por.fl_str_mv |
Vaccine Human T cells Synthetic peptides Schistosoma mansoni Sm14 |
| topic |
Vaccine Human T cells Synthetic peptides Schistosoma mansoni Sm14 |
| description |
Trabalho completo: acesso restrito, p. 204–212 |
| publishDate |
2005 |
| dc.date.issued.fl_str_mv |
2005-02 |
| dc.date.accessioned.fl_str_mv |
2012-08-20T19:09:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://www.repositorio.ufba.br/ri/handle/ri/6607 |
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1286-4579 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 7, n. 2 |
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1286-4579 v. 7, n. 2 |
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http://www.repositorio.ufba.br/ri/handle/ri/6607 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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http://dx.doi.org/10.1016/j.micinf.2004.10.012 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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