Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Agusti,Rosalia
Data de Publicação: 2000
Outros Autores: Couto,Alicia S, Alves,Maria Júlia M, Colli,Walter, Lederkremer,Rosa M de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016
Resumo: Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.
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spelling Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi[14C]-ethanolamine and [3H]-palmitic acid incorporationphospholipidsdesaturated fatty acidsTrypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.Instituto Oswaldo Cruz, Ministério da Saúde2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016Memórias do Instituto Oswaldo Cruz v.95 n.1 2000reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762000000100016info:eu-repo/semantics/openAccessAgusti,RosaliaCouto,Alicia SAlves,Maria Júlia MColli,WalterLederkremer,Rosa M deeng2020-04-25T17:48:20Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:09:31.895Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
title Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
spellingShingle Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
Agusti,Rosalia
[14C]-ethanolamine and [3H]-palmitic acid incorporation
phospholipids
desaturated fatty acids
title_short Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
title_full Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
title_fullStr Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
title_full_unstemmed Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
title_sort Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
author Agusti,Rosalia
author_facet Agusti,Rosalia
Couto,Alicia S
Alves,Maria Júlia M
Colli,Walter
Lederkremer,Rosa M de
author_role author
author2 Couto,Alicia S
Alves,Maria Júlia M
Colli,Walter
Lederkremer,Rosa M de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Agusti,Rosalia
Couto,Alicia S
Alves,Maria Júlia M
Colli,Walter
Lederkremer,Rosa M de
dc.subject.por.fl_str_mv [14C]-ethanolamine and [3H]-palmitic acid incorporation
phospholipids
desaturated fatty acids
topic [14C]-ethanolamine and [3H]-palmitic acid incorporation
phospholipids
desaturated fatty acids
dc.description.none.fl_txt_mv Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.
description Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762000000100016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.95 n.1 2000
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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