Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402006000200010 |
Resumo: | The aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterilie distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37°C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37°C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus. |
id |
FUNORP-1_4963afaa27b7abf4ef5c6423872f2d90 |
---|---|
oai_identifier_str |
oai:scielo:S0103-64402006000200010 |
network_acronym_str |
FUNORP-1 |
network_name_str |
Brazilian Dental Journal |
repository_id_str |
|
spelling |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureusozoneultrasonic cleaner systemwater disinfectionStaphylococcus aureusdental treatment unitsThe aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterilie distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37°C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37°C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus.Fundação Odontológica de Ribeirão Preto2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402006000200010Brazilian Dental Journal v.17 n.2 2006reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402006000200010info:eu-repo/semantics/openAccessEstrela,CarlosEstrela,Cyntia R.A.Decurcio,Daniel de AlmeidaSilva,Julio AlmeidaBammann,Lili Luschkeeng2006-08-14T00:00:00Zoai:scielo:S0103-64402006000200010Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2006-08-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
title |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
spellingShingle |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus Estrela,Carlos ozone ultrasonic cleaner system water disinfection Staphylococcus aureus dental treatment units |
title_short |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
title_full |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
title_fullStr |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
title_full_unstemmed |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
title_sort |
Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus |
author |
Estrela,Carlos |
author_facet |
Estrela,Carlos Estrela,Cyntia R.A. Decurcio,Daniel de Almeida Silva,Julio Almeida Bammann,Lili Luschke |
author_role |
author |
author2 |
Estrela,Cyntia R.A. Decurcio,Daniel de Almeida Silva,Julio Almeida Bammann,Lili Luschke |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Estrela,Carlos Estrela,Cyntia R.A. Decurcio,Daniel de Almeida Silva,Julio Almeida Bammann,Lili Luschke |
dc.subject.por.fl_str_mv |
ozone ultrasonic cleaner system water disinfection Staphylococcus aureus dental treatment units |
topic |
ozone ultrasonic cleaner system water disinfection Staphylococcus aureus dental treatment units |
description |
The aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterilie distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37°C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37°C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402006000200010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402006000200010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402006000200010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.17 n.2 2006 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204090153304064 |