Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ

Detalhes bibliográficos
Autor(a) principal: Cotrim,Paola
Data de Publicação: 2002
Outros Autores: Andrade,Cleverton Roberto de, Line,Sergio, Almeida,Oslei Paes de, Coletta,Ricardo D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200004
Resumo: Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
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spelling Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germmatrix metalloproteinase-2tooth germratTooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.Fundação Odontológica de Ribeirão Preto2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200004Brazilian Dental Journal v.13 n.2 2002reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402002000200004info:eu-repo/semantics/openAccessCotrim,PaolaAndrade,Cleverton Roberto deLine,SergioAlmeida,Oslei Paes deColetta,Ricardo D.eng2003-04-04T00:00:00Zoai:scielo:S0103-64402002000200004Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2003-04-04T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
title Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
spellingShingle Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
Cotrim,Paola
matrix metalloproteinase-2
tooth germ
rat
title_short Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
title_full Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
title_fullStr Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
title_full_unstemmed Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
title_sort Expression and Activity of Matrix Metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ
author Cotrim,Paola
author_facet Cotrim,Paola
Andrade,Cleverton Roberto de
Line,Sergio
Almeida,Oslei Paes de
Coletta,Ricardo D.
author_role author
author2 Andrade,Cleverton Roberto de
Line,Sergio
Almeida,Oslei Paes de
Coletta,Ricardo D.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cotrim,Paola
Andrade,Cleverton Roberto de
Line,Sergio
Almeida,Oslei Paes de
Coletta,Ricardo D.
dc.subject.por.fl_str_mv matrix metalloproteinase-2
tooth germ
rat
topic matrix metalloproteinase-2
tooth germ
rat
description Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402002000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.13 n.2 2002
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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