Phylogenetic analysis of human group C rotavirus in hospitalized children with gastroenteritis in Belém, Brazil

• Autor(a) principal: Lobo, Patrícia dos Santos
• Data de Publicação: 2016
• Outros Autores: Guerra, Sylvia de Fátima dos Santos, Siqueira, Jones Anderson Monteiro, Soares, Luana da Silva, Gabbay, Yvone Benchimol, Linhares, Alexandre da Costa, Mascarenhas, Joana D'Arc Pereira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2423
• topic: Gastroenterite / epidemiologia; Gastroenterite / virologia; Rotavirus / classificação; Rotavirus / genética; Infecções por Rotavirus / epidemiologia; Infecções por Rotavirus / virologia; Genótipo; Criança Hospitalizada; Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos; Região Amazônica (BR)

Group C rotavirus (RVC) is potentially an important pathogen associated with acute gastroenteritis (AG), especially in outbreaks. This study aims to detect and molecularly characterize RVC in hospitalized children with AG in Belém, Brazil. From May 2008 to April 2011, 279 stools were subjected to reverse-transcription polymerase chain reaction targeting VP7, VP6, VP4, and NSP4 genes. RVC positivity rate was 2.1 per cent (6/279) and phylogenetic analysis of positive samples yields genotype G4-P[2]-I2-E2. No evidence of zoonotic transmission and VP7 gene demonstrated close relationship with Asian strains. RVC surveillance is worth to expand information on evolutionary and epidemiological features of this virus.