Phenotypic analysis of yellow fever virus derived from complementary DNA

• Autor(a) principal: Marchevsky, R. S
• Data de Publicação: 1995
• Outros Autores: Mariano, J, Ferreira, V. S, Almeida, E, Cerqueira, M. J, Carvalho, R, Pissurno, J. W, Rosa, Amélia Paes de Andrade Travassos da, Simoes, M. C, Santos, C. N. D, Ferreira, I. I, Muylaert, I. R, Mann, G. F, Rice, C. M, Galler, R
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/337
• topic: Febre Amarela / virologia; Vírus da Febre Amarela / classificação; Vírus da Febre Amarela / genética; Vírus da Febre Amarela / imunologia; Vírus da Febre Amarela / patogenicidade; Células Cultivadas; Sistema Nervoso Central / virologia; Fibroblastos / virologia; Macaca fascicularis; Macaca mulatta; Fenótipo; DNA Viral / fisiologia; Inoculações Seriadas; Transfecção; Vacinas Virais / normas; Embrião de Galinha

A thorough phenotypic characterization of yellow fever (YF) virus generated from cDNA is a necessary prerequisite for mapping virulence/attenuation determinants and exploring the potential of YF attenuated virus 17D as a carrier for heterologous protective epitopes. In this study, YF virus was produced from 17D cDNA clones after lipofectin-mediated RNA transfection of certified primary cultures of chicken embryo fibroblasts (YFiv5.2/SL). This virus was passaged once in embryonated chicken eggs according to current YF vaccine manufacture methodology to produce the experimental virus (YFiv5.2NL). These viruses were characterized in established monkey neurovirulence safety tests and quantitative clinical and histologic scores were derived for each virus. The experimental vaccine viruses (YFiv5.2/SL and VL) compared favorably with another well-known YF vaccine strain (17DD) used as control virus for the histologic score. Although slight1y higher clinical neurovirulence was observed for YFiv5.2 as compared with the 17DD virus, it should not preclude the use of YFiv5.2 for mapping YF virus virulence determinants.