A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000600012 |
Resumo: | The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver. |
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A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazilgenotoxicitypollutionbiomarkersoxidative stresstissue damageThe aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver.Instituto Internacional de Ecologia2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000600012Brazilian Journal of Biology v.70 n.4 suppl.0 2010reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S1519-69842010000600012info:eu-repo/semantics/openAccessRechenmacher,C.Siebel,AM.Goldoni,A.Klauck,CR.Sartori,T.Rodrigues,MT.Rodrigues,MAS.Gehlen,G.Ardenghi,PG.Silva,LBeng2010-12-21T00:00:00Zoai:scielo:S1519-69842010000600012Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2010-12-21T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
title |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
spellingShingle |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil Rechenmacher,C. genotoxicity pollution biomarkers oxidative stress tissue damage |
title_short |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
title_full |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
title_fullStr |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
title_full_unstemmed |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
title_sort |
A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil |
author |
Rechenmacher,C. |
author_facet |
Rechenmacher,C. Siebel,AM. Goldoni,A. Klauck,CR. Sartori,T. Rodrigues,MT. Rodrigues,MAS. Gehlen,G. Ardenghi,PG. Silva,LB |
author_role |
author |
author2 |
Siebel,AM. Goldoni,A. Klauck,CR. Sartori,T. Rodrigues,MT. Rodrigues,MAS. Gehlen,G. Ardenghi,PG. Silva,LB |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rechenmacher,C. Siebel,AM. Goldoni,A. Klauck,CR. Sartori,T. Rodrigues,MT. Rodrigues,MAS. Gehlen,G. Ardenghi,PG. Silva,LB |
dc.subject.por.fl_str_mv |
genotoxicity pollution biomarkers oxidative stress tissue damage |
topic |
genotoxicity pollution biomarkers oxidative stress tissue damage |
description |
The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000600012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000600012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-69842010000600012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.70 n.4 suppl.0 2010 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129878734405632 |