Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000900053 |
Resumo: | The advanced oxidation process (AOP) is used to increase the treatment efficiency of effluents however, it is necessary to compare the toxicity of treated and untreated effluents to evaluate if the decontamination process does not cause any biological harm. Cultured cells have been previously used to assess the genotoxic and cytotoxic potential of various compounds. Hence, the aim of this work was to assess the applicability of cytotoxicity assays to evaluate the toxicity related to the AOP treatment. Samples of an industrial effluent were collected after their treatment by a conventional method. Cytotoxicity of standard and AOP treated effluents was assessed in CRIB and HEp-2 cell line using the MTT and neutral red assays. We observed decrease at cell viability in the both assays (50% MTT and 13% NRU) when cells were exposed to the AOP treatment in the highest concentration. Thus, cytotoxic assays in cultured cells can be explored as an useful method to evaluate toxicity as well as to optimize effluents treatment process. |
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Brazilian Journal of Biology |
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Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidationcytotoxicitycell cultureeffluentphotoelectrooxidationtanneryThe advanced oxidation process (AOP) is used to increase the treatment efficiency of effluents however, it is necessary to compare the toxicity of treated and untreated effluents to evaluate if the decontamination process does not cause any biological harm. Cultured cells have been previously used to assess the genotoxic and cytotoxic potential of various compounds. Hence, the aim of this work was to assess the applicability of cytotoxicity assays to evaluate the toxicity related to the AOP treatment. Samples of an industrial effluent were collected after their treatment by a conventional method. Cytotoxicity of standard and AOP treated effluents was assessed in CRIB and HEp-2 cell line using the MTT and neutral red assays. We observed decrease at cell viability in the both assays (50% MTT and 13% NRU) when cells were exposed to the AOP treatment in the highest concentration. Thus, cytotoxic assays in cultured cells can be explored as an useful method to evaluate toxicity as well as to optimize effluents treatment process.Instituto Internacional de Ecologia2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000900053Brazilian Journal of Biology v.75 n.4 suppl.2 2015reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.01713supplinfo:eu-repo/semantics/openAccessJaeger,N.Moraes,J. P.Klauck,C. R.Gehlen,G.Rodrigues,M. A. S.Ziulkoski,A. L.eng2016-01-18T00:00:00Zoai:scielo:S1519-69842015000900053Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2016-01-18T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
title |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
spellingShingle |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation Jaeger,N. cytotoxicity cell culture effluent photoelectrooxidation tannery |
title_short |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
title_full |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
title_fullStr |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
title_full_unstemmed |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
title_sort |
Cytotoxicity assays to evaluate tannery effluents treated by photoelectrooxidation |
author |
Jaeger,N. |
author_facet |
Jaeger,N. Moraes,J. P. Klauck,C. R. Gehlen,G. Rodrigues,M. A. S. Ziulkoski,A. L. |
author_role |
author |
author2 |
Moraes,J. P. Klauck,C. R. Gehlen,G. Rodrigues,M. A. S. Ziulkoski,A. L. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Jaeger,N. Moraes,J. P. Klauck,C. R. Gehlen,G. Rodrigues,M. A. S. Ziulkoski,A. L. |
dc.subject.por.fl_str_mv |
cytotoxicity cell culture effluent photoelectrooxidation tannery |
topic |
cytotoxicity cell culture effluent photoelectrooxidation tannery |
description |
The advanced oxidation process (AOP) is used to increase the treatment efficiency of effluents however, it is necessary to compare the toxicity of treated and untreated effluents to evaluate if the decontamination process does not cause any biological harm. Cultured cells have been previously used to assess the genotoxic and cytotoxic potential of various compounds. Hence, the aim of this work was to assess the applicability of cytotoxicity assays to evaluate the toxicity related to the AOP treatment. Samples of an industrial effluent were collected after their treatment by a conventional method. Cytotoxicity of standard and AOP treated effluents was assessed in CRIB and HEp-2 cell line using the MTT and neutral red assays. We observed decrease at cell viability in the both assays (50% MTT and 13% NRU) when cells were exposed to the AOP treatment in the highest concentration. Thus, cytotoxic assays in cultured cells can be explored as an useful method to evaluate toxicity as well as to optimize effluents treatment process. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000900053 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000900053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1519-6984.01713suppl |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.75 n.4 suppl.2 2015 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129882082508800 |