Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/189098 |
Resumo: | Many infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks. |
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Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus speciesNeonatal milkHuman milk banksBreastfeedingMultidrug-resistant bacteriaStaphylococcal toxinsMany infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2021-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/18909810.1590/S1678-9946202163054 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e54Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e54Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e541678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/189098/174756Copyright (c) 2021 Tatiana Salerno , Amanda Keller Siqueira, José Paes de Almeida Nogueira Pinto, Maria de Lourdes Ribeiro de Souza da Cunha, Patrícia Kelly Silvestre, Larissa Anuska Zeni Condas , Gustavo Henrique Batista Lara, Juliano Gonçalves Pereira, Aristeu Vieira da Silva, Fernando José Paganini Listoni , Lorrayne de Souza Araújo Martins, Rodrigo Garcia Motta, Márcio Garcia Ribeirohttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSalerno , Tatiana Siqueira, Amanda Keller Pinto, José Paes de Almeida Nogueira Cunha, Maria de Lourdes Ribeiro de Souza da Silvestre, Patrícia Kelly Condas , Larissa Anuska Zeni Lara, Gustavo Henrique Batista Pereira, Juliano Gonçalves Silva, Aristeu Vieira da Listoni , Fernando José Paganini Martins, Lorrayne de Souza Araújo Motta, Rodrigo Garcia Ribeiro, Márcio Garcia 2022-05-16T13:44:35Zoai:revistas.usp.br:article/189098Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:59.018763Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
spellingShingle |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species Salerno , Tatiana Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
title_short |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_full |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_fullStr |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_full_unstemmed |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
title_sort |
Safety issues of raw milk: evaluation of bacteriological and physicochemical characteristics of human milk from a bank in a teaching hospital, focusing on Staphylococcus species |
author |
Salerno , Tatiana |
author_facet |
Salerno , Tatiana Siqueira, Amanda Keller Pinto, José Paes de Almeida Nogueira Cunha, Maria de Lourdes Ribeiro de Souza da Silvestre, Patrícia Kelly Condas , Larissa Anuska Zeni Lara, Gustavo Henrique Batista Pereira, Juliano Gonçalves Silva, Aristeu Vieira da Listoni , Fernando José Paganini Martins, Lorrayne de Souza Araújo Motta, Rodrigo Garcia Ribeiro, Márcio Garcia |
author_role |
author |
author2 |
Siqueira, Amanda Keller Pinto, José Paes de Almeida Nogueira Cunha, Maria de Lourdes Ribeiro de Souza da Silvestre, Patrícia Kelly Condas , Larissa Anuska Zeni Lara, Gustavo Henrique Batista Pereira, Juliano Gonçalves Silva, Aristeu Vieira da Listoni , Fernando José Paganini Martins, Lorrayne de Souza Araújo Motta, Rodrigo Garcia Ribeiro, Márcio Garcia |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Salerno , Tatiana Siqueira, Amanda Keller Pinto, José Paes de Almeida Nogueira Cunha, Maria de Lourdes Ribeiro de Souza da Silvestre, Patrícia Kelly Condas , Larissa Anuska Zeni Lara, Gustavo Henrique Batista Pereira, Juliano Gonçalves Silva, Aristeu Vieira da Listoni , Fernando José Paganini Martins, Lorrayne de Souza Araújo Motta, Rodrigo Garcia Ribeiro, Márcio Garcia |
dc.subject.por.fl_str_mv |
Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
topic |
Neonatal milk Human milk banks Breastfeeding Multidrug-resistant bacteria Staphylococcal toxins |
description |
Many infants are nurtured with milk supplied by human banks, whose bacteriological and physical-chemical profiles are a major issue. We investigated the bacteriological and physical-chemical characteristics, as well as genotypic and phenotypic and profiles of Staphylococcus species isolated from 240 samples of breast milk from a bank in a teaching hospital. Dornic acidity of milk revealed that 95.4% (229/240) had acceptable limits (< 8.0 oD). Caloric intake showed a wide variation in cream content (4%), fat (4%) and energy values (559.81 Kcal/L). Staphylococcus (105/186 or 56.5%) and Enterobacter (25/186 or 13.4%) were the most prevalent genera, although other microorganisms were identified, including Klebsiella pneumoniae and Pseudomonas aeruginosa. Amoxicillin/clavulanic acid (125/157 or 79.6%), vancomycin (115/157 or 73.2%), and cephalexin (112/157 or 71.3%) were the most effective antimicrobials. High resistance rates of isolates were found to penicillin G (141/157 or 89.8%), ampicillin (135/157 or 86%), and oxacillin (118/157 or 75.2%). Multidrug resistance to ≥ 3 antimicrobials occurred in 66.2% (123/186) of the isolates. Residues of microbial multiplication inhibitory substances were found in 85% (204/240) of samples. Among the coagulase-positive-CPS and negative-CoNS staphylococci, the mecA gene was detected in 53.3% (8/15) and 75% (30/40), respectively. Genes sea, seb and sec were detected in 20% (3/15) of CPS, while tsst-1 was detected in 13.34% (2/15). In addition, 13.3% (2/15) of S. aureus were toxin-producers. Genes sea, seb and sec were detected in 90% (36/40), 5% (2/40) and 15% (6/40) CoNS, respectively. Enterotoxin production was identified in 5% (2/40) of CoNS. The identification of multidrug-resistant bacteria, staphylococci species toxin-producers harboring methicillin-resistance genes, and residues of microbial multiplication inhibitory substances reinforce the need for a continuous vigilance of milk quality offered to infant consumption by human banks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/189098 10.1590/S1678-9946202163054 |
url |
https://www.revistas.usp.br/rimtsp/article/view/189098 |
identifier_str_mv |
10.1590/S1678-9946202163054 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/189098/174756 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e54 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e54 Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e54 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951653220548608 |