Atividade anti-helmíntica do lapachol, β-lapachona e derivados contra larvas de Toxocara canis

• Autor(a) principal: MATA-SANTOS, Taís
• Data de Publicação: 2015
• Outros Autores: PINTO, Nitza França, MATA-SANTOS, Hilton Antônio, DE MOURA, Kelly Gallan, CARNEIRO, Paula Fernandes, CARVALHO, Tatiane dos Santos, DEL RIO, Karina Pena, PINTO, Maria do Carmo Freire Ribeiro, MARTINS, Lourdes Rodrigues, FENALTI, Juliana Montelli, DA SILVA, Pedro Eduardo Almeida, SCAINI, Carlos James
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/101009

Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitro activity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds.