Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis

Detalhes bibliográficos
Autor(a) principal: Mariguela, Viviane Casagrande
Data de Publicação: 2008
Outros Autores: Chacha, Silvana Gama Florêncio, Cunha, Aldo de Albuquerque, Troncon, Luiz Ernesto de Almeida, Zucoloto, Sérgio, Figueiredo, Luiz Tadeu Moraes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31157
Resumo: Cytomegalovirus (CMV) is a genus in the family Herpesviridae that has been associated with gastrointestinal syndromes. In this work we looked for a possible association of CMV infection with colorectal cancer and ulcerative colitis (UC). Blood and enteric tissue samples of 14 patients with colorectal cancer and of 21 with UC were subjected to a nested-PCR that amplifies part of the gB gene of CMV and also to immunohistochemistry using a specific monoclonal antibody to IE 76kDa protein of CMV. CMV was detected by nested-PCR in the blood and/or the enteric tissue of nine (64.3%) colorectal cancer and 16 (76.2%) ulcerative colitis patients. In the immunohistochemistry it was observed that 12 (12/21, 57.1%) positive enteric tissue samples of patients with UC and none from patients with colorectal cancer (0/14) were positive to CMV. The positivity of CMV infections in the UC patient group (12/21, 57.1%) showed by both techniques, was significantly higher (p = 0.015) than that observed for colorectal cancer patients (2/14, 14.3%). These results suggest an association of ulcerative colitis with CMV infection of the enteric tissue.
id IMT-1_912c1f2bb7b2d92bfefdec6ca580d019
oai_identifier_str oai:revistas.usp.br:article/31157
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis Citomegalovírus em câncer coloretal e colite idiopática ulcerativa CytomegalovirusColorectal cancerUlcerative colitisPolymerase chain reactionImmunohistochemistry Cytomegalovirus (CMV) is a genus in the family Herpesviridae that has been associated with gastrointestinal syndromes. In this work we looked for a possible association of CMV infection with colorectal cancer and ulcerative colitis (UC). Blood and enteric tissue samples of 14 patients with colorectal cancer and of 21 with UC were subjected to a nested-PCR that amplifies part of the gB gene of CMV and also to immunohistochemistry using a specific monoclonal antibody to IE 76kDa protein of CMV. CMV was detected by nested-PCR in the blood and/or the enteric tissue of nine (64.3%) colorectal cancer and 16 (76.2%) ulcerative colitis patients. In the immunohistochemistry it was observed that 12 (12/21, 57.1%) positive enteric tissue samples of patients with UC and none from patients with colorectal cancer (0/14) were positive to CMV. The positivity of CMV infections in the UC patient group (12/21, 57.1%) showed by both techniques, was significantly higher (p = 0.015) than that observed for colorectal cancer patients (2/14, 14.3%). These results suggest an association of ulcerative colitis with CMV infection of the enteric tissue. Os Cytomegalovírus (CMV) são um gênero da família Herpesviridae, que pode estar associado a síndromes gastrointestinais. No presente trabalho buscamos uma possível associação da infecção por CMV com câncer coloretal e retocolite ulcerativa (RCU). Amostras de sangue e tecido entérico de 14 pacientes com câncer coloretal e 21 com RCU foram submetidas a uma nested-PCR que amplifica parte do gene gB do CMV e a uma imunohistoquímica utilizando um anticorpo monoclonal específico para proteína IE 76Kda de CMV. CMV foi detectado pela nested-PCR em sangue e/ou tecido entérico de 9 (64,3%) dos pacientes com câncer coloretal e 16 (76,2%) dos pacientes com RCU. Na imunohistoquímica foram observadas 12 (57,1%) amostras positivas para CMV nos pacientes com RCU e nos pacientes com câncer coloretal o CMV não foi detectado em nenhuma amostra. A positividade das infecções no grupo de pacientes com RCU (12/21, 57.1%) foi significantemente mais alta (p = 0,015) que aquela observada nos pacientes com câncer coloretal (2/14, 14.3%). Estes resultados sugerem uma associação da presença de CMV no tecido entérico com RCU. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2008-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31157Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 2 (2008); 83-87 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 2 (2008); 83-87 Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 2 (2008); 83-87 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31157/33041Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMariguela, Viviane CasagrandeChacha, Silvana Gama FlorêncioCunha, Aldo de AlbuquerqueTroncon, Luiz Ernesto de AlmeidaZucoloto, SérgioFigueiredo, Luiz Tadeu Moraes2012-07-07T19:08:05Zoai:revistas.usp.br:article/31157Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:50.965450Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
Citomegalovírus em câncer coloretal e colite idiopática ulcerativa
title Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
spellingShingle Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
Mariguela, Viviane Casagrande
Cytomegalovirus
Colorectal cancer
Ulcerative colitis
Polymerase chain reaction
Immunohistochemistry
title_short Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
title_full Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
title_fullStr Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
title_full_unstemmed Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
title_sort Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis
author Mariguela, Viviane Casagrande
author_facet Mariguela, Viviane Casagrande
Chacha, Silvana Gama Florêncio
Cunha, Aldo de Albuquerque
Troncon, Luiz Ernesto de Almeida
Zucoloto, Sérgio
Figueiredo, Luiz Tadeu Moraes
author_role author
author2 Chacha, Silvana Gama Florêncio
Cunha, Aldo de Albuquerque
Troncon, Luiz Ernesto de Almeida
Zucoloto, Sérgio
Figueiredo, Luiz Tadeu Moraes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mariguela, Viviane Casagrande
Chacha, Silvana Gama Florêncio
Cunha, Aldo de Albuquerque
Troncon, Luiz Ernesto de Almeida
Zucoloto, Sérgio
Figueiredo, Luiz Tadeu Moraes
dc.subject.por.fl_str_mv Cytomegalovirus
Colorectal cancer
Ulcerative colitis
Polymerase chain reaction
Immunohistochemistry
topic Cytomegalovirus
Colorectal cancer
Ulcerative colitis
Polymerase chain reaction
Immunohistochemistry
description Cytomegalovirus (CMV) is a genus in the family Herpesviridae that has been associated with gastrointestinal syndromes. In this work we looked for a possible association of CMV infection with colorectal cancer and ulcerative colitis (UC). Blood and enteric tissue samples of 14 patients with colorectal cancer and of 21 with UC were subjected to a nested-PCR that amplifies part of the gB gene of CMV and also to immunohistochemistry using a specific monoclonal antibody to IE 76kDa protein of CMV. CMV was detected by nested-PCR in the blood and/or the enteric tissue of nine (64.3%) colorectal cancer and 16 (76.2%) ulcerative colitis patients. In the immunohistochemistry it was observed that 12 (12/21, 57.1%) positive enteric tissue samples of patients with UC and none from patients with colorectal cancer (0/14) were positive to CMV. The positivity of CMV infections in the UC patient group (12/21, 57.1%) showed by both techniques, was significantly higher (p = 0.015) than that observed for colorectal cancer patients (2/14, 14.3%). These results suggest an association of ulcerative colitis with CMV infection of the enteric tissue.
publishDate 2008
dc.date.none.fl_str_mv 2008-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31157
url https://www.revistas.usp.br/rimtsp/article/view/31157
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31157/33041
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 2 (2008); 83-87
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 2 (2008); 83-87
Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 2 (2008); 83-87
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951646826332160