Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.

Detalhes bibliográficos
Autor(a) principal: Guimarães-Marques, Giselle Moura
Data de Publicação: 2012
Outros Autores: Batista, Jacqueline da Silva, Guimarães, H. M., Naice-Daou, M. V., Lima, M. P., Formiga, Kyara M., Santos, Joselita Maria M., Lima, A. C., Rafael, Míriam Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INPA
Texto Completo: https://repositorio.inpa.gov.br/handle/1/14997
Resumo: The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
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spelling Guimarães-Marques, Giselle MouraBatista, Jacqueline da SilvaGuimarães, H. M.Naice-Daou, M. V.Lima, M. P.Formiga, Kyara M.Santos, Joselita Maria M.Lima, A. C.Rafael, Míriam Silva2020-05-07T14:00:25Z2020-05-07T14:00:25Z2012https://repositorio.inpa.gov.br/handle/1/1499710.4238/2012.December.6.3The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.Volume 11, Número 4, Pags. 4198-4203Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessMicrosatellite DnaAnimalsAnophelesDisease CarrierDna SequenceGeneGene LocusGeneticsGenotypePolymerase Chain ReactionSpecies DifferenceAnimalAnophelesGenes, InsectGenetic LociGenotypeInsect VectorsMicrosatellite RepeatsPolymerase Chain ReactionSequence Analysis, DnaSpecies SpecificityIsolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Researchengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfapplication/pdf426872https://repositorio.inpa.gov.br/bitstream/1/14997/1/artigo-inpa.pdf707e95339bbc1e24f42a178a78b22f1aMD51CC-LICENSElicense_rdfapplication/octet-stream914https://repositorio.inpa.gov.br/bitstream/1/14997/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD521/149972020-07-14 10:30:41.916oai:repositorio:1/14997Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-07-14T14:30:41Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.en.fl_str_mv Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
spellingShingle Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
Guimarães-Marques, Giselle Moura
Microsatellite Dna
Animals
Anopheles
Disease Carrier
Dna Sequence
Gene
Gene Locus
Genetics
Genotype
Polymerase Chain Reaction
Species Difference
Animal
Anopheles
Genes, Insect
Genetic Loci
Genotype
Insect Vectors
Microsatellite Repeats
Polymerase Chain Reaction
Sequence Analysis, Dna
Species Specificity
title_short Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_full Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_fullStr Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_full_unstemmed Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_sort Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
author Guimarães-Marques, Giselle Moura
author_facet Guimarães-Marques, Giselle Moura
Batista, Jacqueline da Silva
Guimarães, H. M.
Naice-Daou, M. V.
Lima, M. P.
Formiga, Kyara M.
Santos, Joselita Maria M.
Lima, A. C.
Rafael, Míriam Silva
author_role author
author2 Batista, Jacqueline da Silva
Guimarães, H. M.
Naice-Daou, M. V.
Lima, M. P.
Formiga, Kyara M.
Santos, Joselita Maria M.
Lima, A. C.
Rafael, Míriam Silva
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães-Marques, Giselle Moura
Batista, Jacqueline da Silva
Guimarães, H. M.
Naice-Daou, M. V.
Lima, M. P.
Formiga, Kyara M.
Santos, Joselita Maria M.
Lima, A. C.
Rafael, Míriam Silva
dc.subject.eng.fl_str_mv Microsatellite Dna
Animals
Anopheles
Disease Carrier
Dna Sequence
Gene
Gene Locus
Genetics
Genotype
Polymerase Chain Reaction
Species Difference
Animal
Anopheles
Genes, Insect
Genetic Loci
Genotype
Insect Vectors
Microsatellite Repeats
Polymerase Chain Reaction
Sequence Analysis, Dna
Species Specificity
topic Microsatellite Dna
Animals
Anopheles
Disease Carrier
Dna Sequence
Gene
Gene Locus
Genetics
Genotype
Polymerase Chain Reaction
Species Difference
Animal
Anopheles
Genes, Insect
Genetic Loci
Genotype
Insect Vectors
Microsatellite Repeats
Polymerase Chain Reaction
Sequence Analysis, Dna
Species Specificity
description The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2020-05-07T14:00:25Z
dc.date.available.fl_str_mv 2020-05-07T14:00:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.inpa.gov.br/handle/1/14997
dc.identifier.doi.none.fl_str_mv 10.4238/2012.December.6.3
url https://repositorio.inpa.gov.br/handle/1/14997
identifier_str_mv 10.4238/2012.December.6.3
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Volume 11, Número 4, Pags. 4198-4203
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nc-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:Repositório Institucional do INPA
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Repositório Institucional do INPA
collection Repositório Institucional do INPA
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