Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Nutrição |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732020000100306 |
Resumo: | ABSTRACT Objective In this paper, we studied three different types of ordinary sports supplements containing whey protein: whey protein-based ones, hypercaloric ones, and protein bars. Methods A sample preparation procedure was studied employing microwave-assisted wet digestion in order to determine the Chromium and Manganese levels by graphite furnace atomic absorption spectrometry. Results The developed methods have presented good accuracy (recoveries in the range of 90% to 109%) and precision (Relative standard deviation <8%). Although an adequate detectability was obtained (50ng g-1 for Manganese and 65ng g-1 for Chromium), the sample preparation method was also adequate to inductively coupled plasma mass spectrometry analysis. The method was applied to 26 commercial samples, in which the Chromium concentrations were in the range between 0.22 and 1.0μg g-1 and the Manganese concentrations varied from 2.0 to 37μg g-1. Conclusion The results obtained by atomic absorption for both analytes were in agreement with those obtained by mass spectrometry. In addition, some samples presented concentrations of Chromium above the recommended daily intake and, as a result, we used the X-ray powder diffraction technique as an analytical tool to evaluate the oxidation state of Chromium in such samples. |
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Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometryAtomic absorptionChromiumManganeseMicronutrientsSupplementsX-ray diffractionABSTRACT Objective In this paper, we studied three different types of ordinary sports supplements containing whey protein: whey protein-based ones, hypercaloric ones, and protein bars. Methods A sample preparation procedure was studied employing microwave-assisted wet digestion in order to determine the Chromium and Manganese levels by graphite furnace atomic absorption spectrometry. Results The developed methods have presented good accuracy (recoveries in the range of 90% to 109%) and precision (Relative standard deviation <8%). Although an adequate detectability was obtained (50ng g-1 for Manganese and 65ng g-1 for Chromium), the sample preparation method was also adequate to inductively coupled plasma mass spectrometry analysis. The method was applied to 26 commercial samples, in which the Chromium concentrations were in the range between 0.22 and 1.0μg g-1 and the Manganese concentrations varied from 2.0 to 37μg g-1. Conclusion The results obtained by atomic absorption for both analytes were in agreement with those obtained by mass spectrometry. In addition, some samples presented concentrations of Chromium above the recommended daily intake and, as a result, we used the X-ray powder diffraction technique as an analytical tool to evaluate the oxidation state of Chromium in such samples.Pontifícia Universidade Católica de Campinas2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732020000100306Revista de Nutrição v.33 2020reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMP10.1590/1678-9865202033e190141info:eu-repo/semantics/openAccessLISBOA,Thalles PedrosaFLORES,Leonã da SilvaCORREA,Charlane CiminiSOUSA,Rafael Arromba deeng2020-04-08T00:00:00Zoai:scielo:S1415-52732020000100306Revistahttp://www.scielo.br/rnONGhttps://old.scielo.br/oai/scielo-oai.php||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2020-04-08T00:00Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
title |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
spellingShingle |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry LISBOA,Thalles Pedrosa Atomic absorption Chromium Manganese Micronutrients Supplements X-ray diffraction |
title_short |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
title_full |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
title_fullStr |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
title_full_unstemmed |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
title_sort |
Evaluation of Chromium and Manganese levels in sports supplements using graphite furnace atomic absorption spectrometry |
author |
LISBOA,Thalles Pedrosa |
author_facet |
LISBOA,Thalles Pedrosa FLORES,Leonã da Silva CORREA,Charlane Cimini SOUSA,Rafael Arromba de |
author_role |
author |
author2 |
FLORES,Leonã da Silva CORREA,Charlane Cimini SOUSA,Rafael Arromba de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
LISBOA,Thalles Pedrosa FLORES,Leonã da Silva CORREA,Charlane Cimini SOUSA,Rafael Arromba de |
dc.subject.por.fl_str_mv |
Atomic absorption Chromium Manganese Micronutrients Supplements X-ray diffraction |
topic |
Atomic absorption Chromium Manganese Micronutrients Supplements X-ray diffraction |
description |
ABSTRACT Objective In this paper, we studied three different types of ordinary sports supplements containing whey protein: whey protein-based ones, hypercaloric ones, and protein bars. Methods A sample preparation procedure was studied employing microwave-assisted wet digestion in order to determine the Chromium and Manganese levels by graphite furnace atomic absorption spectrometry. Results The developed methods have presented good accuracy (recoveries in the range of 90% to 109%) and precision (Relative standard deviation <8%). Although an adequate detectability was obtained (50ng g-1 for Manganese and 65ng g-1 for Chromium), the sample preparation method was also adequate to inductively coupled plasma mass spectrometry analysis. The method was applied to 26 commercial samples, in which the Chromium concentrations were in the range between 0.22 and 1.0μg g-1 and the Manganese concentrations varied from 2.0 to 37μg g-1. Conclusion The results obtained by atomic absorption for both analytes were in agreement with those obtained by mass spectrometry. In addition, some samples presented concentrations of Chromium above the recommended daily intake and, as a result, we used the X-ray powder diffraction technique as an analytical tool to evaluate the oxidation state of Chromium in such samples. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732020000100306 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732020000100306 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-9865202033e190141 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Pontifícia Universidade Católica de Campinas |
publisher.none.fl_str_mv |
Pontifícia Universidade Católica de Campinas |
dc.source.none.fl_str_mv |
Revista de Nutrição v.33 2020 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
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1752128820526186496 |