The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Fisioterapia em Movimento |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-51502016000200305 |
Resumo: | Abstract Introduction: Aging is understood as the sum of all biological, psychological and social changes that occur over the years. Associated with aging we list up the changes of morphological and functional order of the immune system: Immunosenescence. Objective This study's objective was to characterize the effect of a brief exercise program on the profile of cytokines and peripheral blood mononuclear cells of elderly individuals in Manaus, AM, Brazil. Materials and methods: Twelve subjects aged 66.8 (± 3.7) years old on average engaged in three weekly sessions of exercises for 16 weeks and, seven subjects aged 66.1 (± 6.7) years on average, who practiced only recreational activities, composed the control group. Serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α and INF-γ were measured using the CBA technique (cytometric Bead Array) and the count of subpopulations of lymphocytes - B, B1, T/CD4, T/CD8, Treg, NK and NKT - was performed using flow cytometry. Results: The relative number of B lymphocytes, T/CD4+ and NKT (CD3+/CD16 +/CD56+) increased significantly (p <0.05) after physical activity, compared to the pre-exercise phase and the control group. In another analysis, each individual in the test group was classified either as major or minor producer of each cytokine; i.e., their values were above or below the cut-off point defined by the median of all measurements of that cytokine. Patterns of cytokine production were observed in the post-exercise group, which allowed defining sets ("signatures") of cytokines that were associated with the practice of short-term physical exercises. Conclusion: Our work showed that exercise induces changes in the count of immune cells, which allows us to infer that it can be used as an alternative to reverse or mitigate the implications of immunosenescence. |
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The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, AmazonasAgingFitnessCytokineBead-arrayImmunosenescence.Abstract Introduction: Aging is understood as the sum of all biological, psychological and social changes that occur over the years. Associated with aging we list up the changes of morphological and functional order of the immune system: Immunosenescence. Objective This study's objective was to characterize the effect of a brief exercise program on the profile of cytokines and peripheral blood mononuclear cells of elderly individuals in Manaus, AM, Brazil. Materials and methods: Twelve subjects aged 66.8 (± 3.7) years old on average engaged in three weekly sessions of exercises for 16 weeks and, seven subjects aged 66.1 (± 6.7) years on average, who practiced only recreational activities, composed the control group. Serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α and INF-γ were measured using the CBA technique (cytometric Bead Array) and the count of subpopulations of lymphocytes - B, B1, T/CD4, T/CD8, Treg, NK and NKT - was performed using flow cytometry. Results: The relative number of B lymphocytes, T/CD4+ and NKT (CD3+/CD16 +/CD56+) increased significantly (p <0.05) after physical activity, compared to the pre-exercise phase and the control group. In another analysis, each individual in the test group was classified either as major or minor producer of each cytokine; i.e., their values were above or below the cut-off point defined by the median of all measurements of that cytokine. Patterns of cytokine production were observed in the post-exercise group, which allowed defining sets ("signatures") of cytokines that were associated with the practice of short-term physical exercises. Conclusion: Our work showed that exercise induces changes in the count of immune cells, which allows us to infer that it can be used as an alternative to reverse or mitigate the implications of immunosenescence.Pontifícia Universidade Católica do Paraná2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-51502016000200305Fisioterapia em Movimento v.29 n.2 2016reponame:Fisioterapia em Movimentoinstname:Pontifícia Universidade Católica do Paraná (PUC-PR)instacron:PUC_PR10.1590/0103-5150.029.002.AO09info:eu-repo/semantics/openAccessAnjos,Márcia Cristina Gomes dosPimentel,João Paulo DinizCosta,Allysson Guimarães daEpifânio,Deiziane de SouzaMalheiro,AdrianaPassos,Luiz Fernando de Souzaeng2016-08-02T00:00:00Zoai:scielo:S0103-51502016000200305Revistahttps://periodicos.pucpr.br/fisioPUBhttps://periodicos.pucpr.br/fisio/oairubia.farias@pucpr.br||revista.fisioterapia@pucpr.br1980-59180103-5150opendoar:2016-08-02T00:00Fisioterapia em Movimento - Pontifícia Universidade Católica do Paraná (PUC-PR)false |
dc.title.none.fl_str_mv |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
title |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
spellingShingle |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas Anjos,Márcia Cristina Gomes dos Aging Fitness Cytokine Bead-array Immunosenescence. |
title_short |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
title_full |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
title_fullStr |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
title_full_unstemmed |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
title_sort |
The effects of brief physical conditioning on immune cells and cytokines in elderly individuals in Manaus, Amazonas |
author |
Anjos,Márcia Cristina Gomes dos |
author_facet |
Anjos,Márcia Cristina Gomes dos Pimentel,João Paulo Diniz Costa,Allysson Guimarães da Epifânio,Deiziane de Souza Malheiro,Adriana Passos,Luiz Fernando de Souza |
author_role |
author |
author2 |
Pimentel,João Paulo Diniz Costa,Allysson Guimarães da Epifânio,Deiziane de Souza Malheiro,Adriana Passos,Luiz Fernando de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Anjos,Márcia Cristina Gomes dos Pimentel,João Paulo Diniz Costa,Allysson Guimarães da Epifânio,Deiziane de Souza Malheiro,Adriana Passos,Luiz Fernando de Souza |
dc.subject.por.fl_str_mv |
Aging Fitness Cytokine Bead-array Immunosenescence. |
topic |
Aging Fitness Cytokine Bead-array Immunosenescence. |
description |
Abstract Introduction: Aging is understood as the sum of all biological, psychological and social changes that occur over the years. Associated with aging we list up the changes of morphological and functional order of the immune system: Immunosenescence. Objective This study's objective was to characterize the effect of a brief exercise program on the profile of cytokines and peripheral blood mononuclear cells of elderly individuals in Manaus, AM, Brazil. Materials and methods: Twelve subjects aged 66.8 (± 3.7) years old on average engaged in three weekly sessions of exercises for 16 weeks and, seven subjects aged 66.1 (± 6.7) years on average, who practiced only recreational activities, composed the control group. Serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α and INF-γ were measured using the CBA technique (cytometric Bead Array) and the count of subpopulations of lymphocytes - B, B1, T/CD4, T/CD8, Treg, NK and NKT - was performed using flow cytometry. Results: The relative number of B lymphocytes, T/CD4+ and NKT (CD3+/CD16 +/CD56+) increased significantly (p <0.05) after physical activity, compared to the pre-exercise phase and the control group. In another analysis, each individual in the test group was classified either as major or minor producer of each cytokine; i.e., their values were above or below the cut-off point defined by the median of all measurements of that cytokine. Patterns of cytokine production were observed in the post-exercise group, which allowed defining sets ("signatures") of cytokines that were associated with the practice of short-term physical exercises. Conclusion: Our work showed that exercise induces changes in the count of immune cells, which allows us to infer that it can be used as an alternative to reverse or mitigate the implications of immunosenescence. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-51502016000200305 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-51502016000200305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-5150.029.002.AO09 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Pontifícia Universidade Católica do Paraná |
publisher.none.fl_str_mv |
Pontifícia Universidade Católica do Paraná |
dc.source.none.fl_str_mv |
Fisioterapia em Movimento v.29 n.2 2016 reponame:Fisioterapia em Movimento instname:Pontifícia Universidade Católica do Paraná (PUC-PR) instacron:PUC_PR |
instname_str |
Pontifícia Universidade Católica do Paraná (PUC-PR) |
instacron_str |
PUC_PR |
institution |
PUC_PR |
reponame_str |
Fisioterapia em Movimento |
collection |
Fisioterapia em Movimento |
repository.name.fl_str_mv |
Fisioterapia em Movimento - Pontifícia Universidade Católica do Paraná (PUC-PR) |
repository.mail.fl_str_mv |
rubia.farias@pucpr.br||revista.fisioterapia@pucpr.br |
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1752128634280214528 |