In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/3714 |
Resumo: | ABSTRACT: This is a follow-up study of physicochemical water monitoring data from the abandoned Freixeda gold mine in Portugal, where arsenic (As) has remained above drinking water and irrigation limits over the years. The main objective of the current work was to investigate the toxicological potential of As-containing water on human cell line as an indicator of a potential health risk to humans. Six water samples collected in February 2018 were analysed for arsenic, major anions, cations and trace elements. Toxicity experiments were carried out on the human gastrointestinal cell line Caco-2 with five water samples containing As above 10 μg L−1. The results show that groundwater contains higher amounts of dissolved minerals than surface water, particularly with higher concentrations of SO42−, Fe and HCO3− and also higher As(III), reaching 336 μg L−1 (As(T) = 607 μg L−1). In surface waters As concentration decreased and reached 150 μg L−1, mainly as As(V). Metabolic activity was generally lower in Caco-2 cells exposed to As-containing water samples compared to pure As(III) solution, adapted to As concentrations, while production of reactive oxygen species (ROS) was higher. Short-term exposure to As-contaminated water samples also resulted in increased genotoxicity. This study suggests that mixture of As with various chemical elements in water may have a synergistic effect in promoting cytotoxicity. It is likely that prolonged exposure, as is common in areas with contaminated water, would have even more harmful effects. |
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In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast PortugalArsenicWater contaminationCytotoxicityGenotoxicityCaco-2 cellsABSTRACT: This is a follow-up study of physicochemical water monitoring data from the abandoned Freixeda gold mine in Portugal, where arsenic (As) has remained above drinking water and irrigation limits over the years. The main objective of the current work was to investigate the toxicological potential of As-containing water on human cell line as an indicator of a potential health risk to humans. Six water samples collected in February 2018 were analysed for arsenic, major anions, cations and trace elements. Toxicity experiments were carried out on the human gastrointestinal cell line Caco-2 with five water samples containing As above 10 μg L−1. The results show that groundwater contains higher amounts of dissolved minerals than surface water, particularly with higher concentrations of SO42−, Fe and HCO3− and also higher As(III), reaching 336 μg L−1 (As(T) = 607 μg L−1). In surface waters As concentration decreased and reached 150 μg L−1, mainly as As(V). Metabolic activity was generally lower in Caco-2 cells exposed to As-containing water samples compared to pure As(III) solution, adapted to As concentrations, while production of reactive oxygen species (ROS) was higher. Short-term exposure to As-contaminated water samples also resulted in increased genotoxicity. This study suggests that mixture of As with various chemical elements in water may have a synergistic effect in promoting cytotoxicity. It is likely that prolonged exposure, as is common in areas with contaminated water, would have even more harmful effects.ElsevierRepositório do LNEGCostaGošar, DorotejaPinti, MarikaFerreira, AdelaideMarušič, Martina Bergant2023-07-01T00:30:19Z2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3714engCosta et al. 2021, ‘In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal’, Environmental Research, vol. 202, article 111683. doi:10.1016/j.envres.2021.1116830013-9351https://doi.org/10.1016/j.envres.2021.111683info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-02T06:25:53ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
title |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
spellingShingle |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal Costa Arsenic Water contamination Cytotoxicity Genotoxicity Caco-2 cells |
title_short |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
title_full |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
title_fullStr |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
title_full_unstemmed |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
title_sort |
In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal |
author |
Costa |
author_facet |
Costa Gošar, Doroteja Pinti, Marika Ferreira, Adelaide Marušič, Martina Bergant |
author_role |
author |
author2 |
Gošar, Doroteja Pinti, Marika Ferreira, Adelaide Marušič, Martina Bergant |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Costa Gošar, Doroteja Pinti, Marika Ferreira, Adelaide Marušič, Martina Bergant |
dc.subject.por.fl_str_mv |
Arsenic Water contamination Cytotoxicity Genotoxicity Caco-2 cells |
topic |
Arsenic Water contamination Cytotoxicity Genotoxicity Caco-2 cells |
description |
ABSTRACT: This is a follow-up study of physicochemical water monitoring data from the abandoned Freixeda gold mine in Portugal, where arsenic (As) has remained above drinking water and irrigation limits over the years. The main objective of the current work was to investigate the toxicological potential of As-containing water on human cell line as an indicator of a potential health risk to humans. Six water samples collected in February 2018 were analysed for arsenic, major anions, cations and trace elements. Toxicity experiments were carried out on the human gastrointestinal cell line Caco-2 with five water samples containing As above 10 μg L−1. The results show that groundwater contains higher amounts of dissolved minerals than surface water, particularly with higher concentrations of SO42−, Fe and HCO3− and also higher As(III), reaching 336 μg L−1 (As(T) = 607 μg L−1). In surface waters As concentration decreased and reached 150 μg L−1, mainly as As(V). Metabolic activity was generally lower in Caco-2 cells exposed to As-containing water samples compared to pure As(III) solution, adapted to As concentrations, while production of reactive oxygen species (ROS) was higher. Short-term exposure to As-contaminated water samples also resulted in increased genotoxicity. This study suggests that mixture of As with various chemical elements in water may have a synergistic effect in promoting cytotoxicity. It is likely that prolonged exposure, as is common in areas with contaminated water, would have even more harmful effects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07 2021-07-01T00:00:00Z 2023-07-01T00:30:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/3714 |
url |
http://hdl.handle.net/10400.9/3714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa et al. 2021, ‘In vitro toxicity of arsenic rich waters from an abandoned gold mine in northeast Portugal’, Environmental Research, vol. 202, article 111683. doi:10.1016/j.envres.2021.111683 0013-9351 https://doi.org/10.1016/j.envres.2021.111683 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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1777301374952800256 |