Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies

Detalhes bibliográficos
Autor(a) principal: Correia, Catarina Ribeiro
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10988
Resumo: Dissertation to obtain a Master Degree in Molecular Genetics and Biomedicine
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spelling Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologiesAeromonasCytotoxicityAdherenceInvasionCaco-2 CellsDissertation to obtain a Master Degree in Molecular Genetics and BiomedicineAims: To contribute to the evaluation of the pathogenic potential of Aeromonas, through the test of 24 Aeromonas spp. strains of Portuguese origin, for the adherence, invasion and cytotoxicity abilities in mammal cells. Rationale: Studies on other enteropathogens indicate that a pathogen must be able to attach to host target cells to cause gastrointestinal disease [Finlay and Falkow, 1997; Scoglio et al., 2001], via either toxin production or host cell invasion, or both [Knutton, et al. 1987]. Results: 19 (79%) and 12 (50%) strains were found to have the ability to adhere and invade differentiated cells respectively while 22 (92%) and 13 (54%) strains had ability to adhere and invade undifferentiated Caco-2 cells. These results indicate that most Aeromonas spp. strains interact optimally with cultured human intestinal cells at cellular sites expressed in the brush border early in the differentiation process of Caco-2 cells. In 13 (54%) strains it was observed an aggregative adhesion pattern as observed in other enteropathogens, including all clinical strains. 6 (25%) isolates express both adherence and extracellular cytotoxicity, but preheating caused a decrease in the citotoxicity of the supernatants of 5 of these strains suggesting that the remainder clinical strain (A255) has the ability to produce extracellular heat-stable toxins. 17 (71%) isolates express cell contact dependent cytotoxicity, but only 13 of these strains were able to invade Caco-2 cells, indicating the presence of others mechanisms of cell lysis not yet determined. Conclusions: Aeromonas spp. strains isolated from water, food and food processing surfaces showed adhesive, invasive and cytotoxic patterns similar or larger than clinical strains, suggesting that environmental Aeromonas spp. stains have the potential to cause human illness and that food and water sources may act as dissemination vehicles of this human pathogen with implication in the public health in Portugal.Faculdade de Ciências e TecnologiaSimplício, AnaCrespo, Maria TeresaRUNCorreia, Catarina Ribeiro2014-01-13T14:58:39Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10988enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:30:13ZPortal AgregadorONG
dc.title.none.fl_str_mv Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
title Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
spellingShingle Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
Correia, Catarina Ribeiro
Aeromonas
Cytotoxicity
Adherence
Invasion
Caco-2 Cells
title_short Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
title_full Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
title_fullStr Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
title_full_unstemmed Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
title_sort Evaluation of pathogenic potential of Aeromonas spp. strains using in vitro methodologies
author Correia, Catarina Ribeiro
author_facet Correia, Catarina Ribeiro
author_role author
dc.contributor.none.fl_str_mv Simplício, Ana
Crespo, Maria Teresa
RUN
dc.contributor.author.fl_str_mv Correia, Catarina Ribeiro
dc.subject.por.fl_str_mv Aeromonas
Cytotoxicity
Adherence
Invasion
Caco-2 Cells
topic Aeromonas
Cytotoxicity
Adherence
Invasion
Caco-2 Cells
description Dissertation to obtain a Master Degree in Molecular Genetics and Biomedicine
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-01-13T14:58:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10988
url http://hdl.handle.net/10362/10988
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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