Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo

Detalhes bibliográficos
Autor(a) principal: Moreira, Carolina G. A.
Data de Publicação: 2013
Outros Autores: Jacinto, Antonio, Prag, Soren
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49920
Resumo: © 2013. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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spelling Drosophila integrin adhesion complexes are essential for hemocyte migration in vivoDrosophilaHemocyteIntegrinMigration© 2013. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms.The work was supported by a fellowship (SFRH/BD/62345/2009) to C.G.A.M. and a grant (PTDC/SAU-OBD/101259/2008) from Fundação para a Ciência e Tecnologia, and a European Research Council Starting Grant (2007-StG-208631).The Company of BiologistsRepositório da Universidade de LisboaMoreira, Carolina G. A.Jacinto, AntonioPrag, Soren2021-10-18T11:06:25Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49920engBiol Open. 2013 Jun 6;2(8):795-80110.1242/bio.201345642046-6390info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:53Zoai:repositorio.ul.pt:10451/49920Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:26.713646Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
spellingShingle Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
Moreira, Carolina G. A.
Drosophila
Hemocyte
Integrin
Migration
title_short Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_full Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_fullStr Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_full_unstemmed Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_sort Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
author Moreira, Carolina G. A.
author_facet Moreira, Carolina G. A.
Jacinto, Antonio
Prag, Soren
author_role author
author2 Jacinto, Antonio
Prag, Soren
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Moreira, Carolina G. A.
Jacinto, Antonio
Prag, Soren
dc.subject.por.fl_str_mv Drosophila
Hemocyte
Integrin
Migration
topic Drosophila
Hemocyte
Integrin
Migration
description © 2013. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2021-10-18T11:06:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49920
url http://hdl.handle.net/10451/49920
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biol Open. 2013 Jun 6;2(8):795-801
10.1242/bio.20134564
2046-6390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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