Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo

Detalhes bibliográficos
Autor(a) principal: Moreira, Carolina G A
Data de Publicação: 2013
Outros Autores: Jacinto, Antonio, Prag, Soren
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1242/bio.20134564
Resumo: We thank Angela Dias for the technical help and the Jacinto group members for insightful discussions. The work was supported by a fellowship (SFRH/BD/62345/2009) to C.G.A.M. and a grant (PTDC/SAU-OBD/101259/2008) from Fundacao para a Ciencia e Tecnologia, and a European Research Council Starting Grant (2007-StG-208631).
id RCAP_d1084660e116d89b3ad26e88c5233c18
oai_identifier_str oai:run.unl.pt:10362/20836
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Drosophila integrin adhesion complexes are essential for hemocyte migration in vivoDrosophilaHemocyteIntegrinMigrationBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)We thank Angela Dias for the technical help and the Jacinto group members for insightful discussions. The work was supported by a fellowship (SFRH/BD/62345/2009) to C.G.A.M. and a grant (PTDC/SAU-OBD/101259/2008) from Fundacao para a Ciencia e Tecnologia, and a European Research Council Starting Grant (2007-StG-208631).Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMoreira, Carolina G AJacinto, AntonioPrag, Soren2017-05-30T14:02:31Z2013-08-152013-08-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttps://doi.org/10.1242/bio.20134564eng2046-6390PURE: 1674418http://www.scopus.com/inward/record.url?scp=84965131597&partnerID=8YFLogxKhttps://doi.org/10.1242/bio.20134564info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:06:43Zoai:run.unl.pt:10362/20836Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:26:30.361730Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
spellingShingle Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
Moreira, Carolina G A
Drosophila
Hemocyte
Integrin
Migration
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
title_short Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_full Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_fullStr Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_full_unstemmed Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
title_sort Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
author Moreira, Carolina G A
author_facet Moreira, Carolina G A
Jacinto, Antonio
Prag, Soren
author_role author
author2 Jacinto, Antonio
Prag, Soren
author2_role author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Moreira, Carolina G A
Jacinto, Antonio
Prag, Soren
dc.subject.por.fl_str_mv Drosophila
Hemocyte
Integrin
Migration
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
topic Drosophila
Hemocyte
Integrin
Migration
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
description We thank Angela Dias for the technical help and the Jacinto group members for insightful discussions. The work was supported by a fellowship (SFRH/BD/62345/2009) to C.G.A.M. and a grant (PTDC/SAU-OBD/101259/2008) from Fundacao para a Ciencia e Tecnologia, and a European Research Council Starting Grant (2007-StG-208631).
publishDate 2013
dc.date.none.fl_str_mv 2013-08-15
2013-08-15T00:00:00Z
2017-05-30T14:02:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1242/bio.20134564
url https://doi.org/10.1242/bio.20134564
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-6390
PURE: 1674418
http://www.scopus.com/inward/record.url?scp=84965131597&partnerID=8YFLogxK
https://doi.org/10.1242/bio.20134564
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137894782205952

Registros relacionados