Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae

Detalhes bibliográficos
Autor(a) principal: Simões, Maria L.
Data de Publicação: 2015
Outros Autores: Gonçalves, Luzia, Silveira, Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1186/s13071-014-0619-y
Resumo: PMID: 25573379 WOS:000349413600001
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spelling Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiaeAnophelesHemozoinInnate immunityParasite-vector interactionPlasmodiumREL2ParasitologyInfectious DiseasesImmunologySDG 3 - Good Health and Well-beingPMID: 25573379 WOS:000349413600001Background: Malaria is a worldwide infectious disease caused by Plasmodium parasites and transmitted by female Anopheles mosquitoes. The malaria vector mosquito Anopheles can trigger effective mechanisms to control completion of the Plasmodium lifecycle; the mosquito immune response to the parasite involves several pathways which are not yet well characterized. Plasmodium metabolite hemozoin has emerged as a potent immunostimulator of mammalian tissues. In this study, we aim to investigate the role of this parasite's by-product as stimulator of Anopheles gambiae immunity to Plasmodium berghei. Methods: Female mosquitoes were inoculated with hemozoin and the Plasmodium infection rate and intensity were measured. Differences between treatments were detected by Zero-inflated models. Microarray transcription analysis was performed to assess gene expression response to hemozoin. Genome-wide analysis results were confirmed by stimulation of Anopheles gambiae tissues and cells with hemozoin and silencing of REL2-F and its negative regulator Caspar. Results: Gene expression profiles revealed that hemozoin activates several immunity genes, including pattern recognition receptors (PRRs) and antimicrobial peptides (AMPs). Importantly, we found that the Immune deficiency (Imd) pathway Nuclear Factor-kappaB (NF-κB) transcription factor REL2, in its full-length form REL2-F, was induced upon hemozoin treatment. Conclusions: We have for the first time shown the impact of hemozoin treatment in Plasmodium infection, reducing both rate and intensity of the infection. We propose that hemozoin boosts the innate immunity in Anopheles, activating key effector genes involved in mosquito resistance to Plasmodium, and this activation is REL2-mediated.Population health, policies and services (PPS)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)RUNSimões, Maria L.Gonçalves, LuziaSilveira, Henrique2018-05-14T22:02:35Z2015-01-082015-01-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1186/s13071-014-0619-yengPURE: 1732679http://www.scopus.com/inward/record.url?scp=84939807789&partnerID=8YFLogxKhttps://doi.org/10.1186/s13071-014-0619-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:30Zoai:run.unl.pt:10362/36949Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:43.536209Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
title Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
spellingShingle Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
Simões, Maria L.
Anopheles
Hemozoin
Innate immunity
Parasite-vector interaction
Plasmodium
REL2
Parasitology
Infectious Diseases
Immunology
SDG 3 - Good Health and Well-being
title_short Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
title_full Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
title_fullStr Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
title_full_unstemmed Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
title_sort Hemozoin activates the innate immune system and reduces Plasmodium berghei infection in Anopheles gambiae
author Simões, Maria L.
author_facet Simões, Maria L.
Gonçalves, Luzia
Silveira, Henrique
author_role author
author2 Gonçalves, Luzia
Silveira, Henrique
author2_role author
author
dc.contributor.none.fl_str_mv Population health, policies and services (PPS)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Simões, Maria L.
Gonçalves, Luzia
Silveira, Henrique
dc.subject.por.fl_str_mv Anopheles
Hemozoin
Innate immunity
Parasite-vector interaction
Plasmodium
REL2
Parasitology
Infectious Diseases
Immunology
SDG 3 - Good Health and Well-being
topic Anopheles
Hemozoin
Innate immunity
Parasite-vector interaction
Plasmodium
REL2
Parasitology
Infectious Diseases
Immunology
SDG 3 - Good Health and Well-being
description PMID: 25573379 WOS:000349413600001
publishDate 2015
dc.date.none.fl_str_mv 2015-01-08
2015-01-08T00:00:00Z
2018-05-14T22:02:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1186/s13071-014-0619-y
url https://doi.org/10.1186/s13071-014-0619-y
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 1732679
http://www.scopus.com/inward/record.url?scp=84939807789&partnerID=8YFLogxK
https://doi.org/10.1186/s13071-014-0619-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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