Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity

Detalhes bibliográficos
Autor(a) principal: Graça, Vânia C.
Data de Publicação: 2017
Outros Autores: Barros, Lillian, Calhelha, Ricardo C., Dias, Maria Inês, Ferreira, Isabel C.F.R., Santos, Paulo F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/15069
Resumo: Geranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum.
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spelling Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activityGeranium robertianum L.FractionationAntioxidant activityAntiproliferative activityPhenolic compoundsGeranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum.This work was supported by European Investment Funds by FEDER/COMPETE/POCI- Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT − Portuguese Foundation for Science and Technology under the projects UID/AGR/04033, UID/AGR/00690/2013 (CIMO) and UID/QUI/00616/2013 (CQ-VR). V.C. Graça, M.I. Dias and L. Barros thank FCT for SFRH/BD/52542/2014, SFRH/BD/84485/2012, and SFRH/BPD/107855/2015 grants, respectively.Biblioteca Digital do IPBGraça, Vânia C.Barros, LillianCalhelha, Ricardo C.Dias, Maria InêsFerreira, Isabel C.F.R.Santos, Paulo F.2018-01-25T10:00:00Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15069engGraça, V.C.; Barros, Lillian; Calhelha, Ricardo C.; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Santos, P.F. (2017). Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity. Industrial Crops & Products. ISSN 0926-6690. 108, p. 543-5520926669010.1016/j.indcrop.2017.07.016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:36Zoai:bibliotecadigital.ipb.pt:10198/15069Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:51.926853Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
title Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
spellingShingle Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
Graça, Vânia C.
Geranium robertianum L.
Fractionation
Antioxidant activity
Antiproliferative activity
Phenolic compounds
title_short Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
title_full Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
title_fullStr Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
title_full_unstemmed Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
title_sort Bio-guided fractionation of extracts of Geranium robertianum L.: relationship between phenolic profile and biological activity
author Graça, Vânia C.
author_facet Graça, Vânia C.
Barros, Lillian
Calhelha, Ricardo C.
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Santos, Paulo F.
author_role author
author2 Barros, Lillian
Calhelha, Ricardo C.
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Santos, Paulo F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Graça, Vânia C.
Barros, Lillian
Calhelha, Ricardo C.
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Santos, Paulo F.
dc.subject.por.fl_str_mv Geranium robertianum L.
Fractionation
Antioxidant activity
Antiproliferative activity
Phenolic compounds
topic Geranium robertianum L.
Fractionation
Antioxidant activity
Antiproliferative activity
Phenolic compounds
description Geranium robertianum L. is used in folk medicine and herbalism practice for the treatment of a variety of ailments. Recently, we studied the bioactivity of several aqueous and organic extracts of this plant. In this work, the more active extracts were fractionated and the fractions evaluated for their antioxidant activity and cytotoxicity against several human tumor cell lines and non-tumor porcine liver primary cells. Some of the fractions from the acetone extract consistently displayed low EC 50 and GI 50 values and presented the higher contents of total phenolic compounds in comparison to other fractions. The phenolic compounds profile of the fractions was determined. The bio-guided fractionation of the extracts resulted in several fractions with improved bioactivity relative to the corresponding extracts. Their lower compositional complexity allowed the identification of more than two dozen compounds, to the best of our knowledge, so far not reported in G. robertianum.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-01-25T10:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/15069
url http://hdl.handle.net/10198/15069
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Graça, V.C.; Barros, Lillian; Calhelha, Ricardo C.; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Santos, P.F. (2017). Bio-guided fractionation of extracts of Geranium robertianum L.: Relationship between phenolic profile and biological activity. Industrial Crops & Products. ISSN 0926-6690. 108, p. 543-552
09266690
10.1016/j.indcrop.2017.07.016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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